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Anti-Osteopontin antibody
Anti-Osteopontin antibody from Abcam
Be the first to write a review!
Citations:
(369)
Supplier Page
Supplier Page from
Abcam for
Anti-Osteopontin antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Osteopontin antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab8448
Antibody Type
Primary
Quantity
200 UL
Isotype
IgG
Host
Rabbit
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/Tag
Unconjugated
Gene Name
SPP1
Reactivity
Human
Target
Osteopontin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (369)
(1)
Development of 3D Printable Calcium Phosphate Cement Scaffolds with Cockle Shell Powders
Materials (Basel)
September 10, 2023
Eunbee Cho, Jae Eun Kim, Juo Lee, Sangbae Park, Sungmin Lee, Jong Hoon Chung, Jungsil Kim, Hoon Seonwoo
phalloidin (Millipore, Burlington, MA, USA) for 1 h. Monoclonal anti-osteopontin (OPN) antibody (
1:500
,
ab8448
,
Abcam
, Cambridge, UK) and FITC-conjugated
goat
anti-
human
antibody were used to stain the OPN protein
(2)
Bioprinted constructs that simulate nerve-bone crosstalk to improve microenvironment for bone repair
Bioactive materials
September 1, 2023
Wang T, Li W, Zhang Y, Xu X, Qiang L, Miao W, Yue X, Jiao X, Zhou X, Ma Z,
et al
.
paraformaldehyde, and then incubated overnight with
Runx2
(
1:1600
,
12556
,
CST
, USA) and OPN (
1:1600
,
ab8448
,
Abcam
, UK) antibodies.
Cytoskeleton
staining
was carried out using
goat anti-rabbit IgG H&L
(
1:1000
,
(3)
Hydroxyapatite-filled osteoinductive and piezoelectric nanofibers for bone tissue engineering
Sci Technol Adv Mater
August 28, 2023
Frederico Barbosa, Fábio F. F. Garrudo, Paola S. Alberte, Leonor Resina, Marta S. Carvalho, Akhil Jain, Ana C. Marques, Francesc Estrany, Frankie J. Rawson, Carlos Aléman,
et al.
Solutions containing primary antibodies for type I collagen (
MA1–26771
,
Thermo
-Fischer) and osteopontin (
ab8448
,
Abcam
) (
1:200
in 1% BSA, 10% FBS, 0.03% Triton X-100 solution) were then incubated with the respective
(4)
Two different isoforms of osteopontin modulate myelination and axonal integrity
FASEB bioAdvances
August 1, 2023
Nilsson G, Mottahedin A, Zelco A, Lauschke VM, Ek CJ, Song J, Ardalan M, Hua S, Zhang X, Mallard C,
et al
.
blocking, the sections were incubated with anti‐
Olig2
(
AF2418
,
R&D Systems
, 5 μg/mL) and anti‐OPN (
Ab8448
,
Abcam
,
1:250
) antibodies followed by
incubation
with appropriate Alexa Fluor‐conjugated secondary antibodies
(5)
Effects of Progestin on Modulation of the Expression of Biomarkers in Endometriosis
Biomedicines
July 20, 2023
Matasariu DR, Bausic AIG, Mandici CE, Bujor IE, Cristofor AE, Bratila E, Lozneanu L, Boiculese LV, Grigore M, Ursache A.
polyclonal IgG isotype,
Abcam
(
ab157107
)",
1:250
,Nuclear Anti—OPN,"Rabbit polyclonal IgG isotype,
Abcam
(
ab8448
)",
1:200
,Nuclear
Immunohistochemical
panel of antibodies used in the study. OPN: osteopontin; IgG:
(6)
Fabrication and Evaluation of Porous dECM/PCL Scaffolds for Bone Tissue Engineering
Journal of Functional Biomaterials
June 29, 2023
Weiwei Wang, Xiaqing Zhou, Zhuozhuo Yin, Xiaojun Yu
containing 3% BSA for 30 min. Afterward, the samples were incubated with the osteopontin (
Abcam
, Boston, MA
ab8448
, from rabbits) or Collagen Type I (
Abcam
,
ab292
, from rabbits) antibodies for 1 h at room temperature
(7)
Molecular signaling from microglia impacts macroglia autophagy and neurons survival in glaucoma
iScience
June 16, 2023
Huan Yu, Huimin Zhong, Jun Sun, Na Li, Junjue Chen, Bingqiao Shen, Ping Huang, Xi Shen, Shouyue Huang, Yisheng Zhong
dilution;
Abcam
,
ab125212
), Iba-1 (
1:1000
dilution;
Abcam
,
ab178846
), OPN (
1:1000
dilution;
Abcam
,
ab8448
),
CD44
(
1:5000
dilution; Abcam,
ab157107
), Itgαvβ3 (
1:5000
dilution; Abcam,
ab179475
),
P62
(
1:1000
dilution;
(8)
Influence of Occlusal Hypofunction on Alveolar Bone Healing in Rats
International Journal of Molecular Sciences
June 5, 2023
Anna Damanaki, Svenja Beisel-Memmert, Marjan Nokhbehsaim, Ali Abedi, Birgit Rath-Deschner, Andressa V. B. Nogueira, James Deschner
polyclonal antibody against osteopontin with a concentration of
1:300
for 1 h at room temperature (
ab8448
,
Abcam
) and with a
mouse
monoclonal antibody against osteocalcin with a concentration of
1:1,200
overnight
(9)
Blockage of Osteopontin‐Integrin β3 Signaling in Infrapatellar Fat Pad Attenuates Osteoarthritis in Mice
Advanced Science
May 23, 2023
Bingyang Dai, Yuwei Zhu, Xu Li, Zuru Liang, Shunxiang Xu, Shian Zhang, Zhe Zhang, Shanshan Bai, Wenxue Tong, Mingde Cao,
et al.
30 min at room temperature. Primary antibodies were incubated that recognized
OPN
(
Abcam
, UK, cat#
ab8448
,
1:100
),
MMP9
(
Abclonal
, USA, cat#
A2095
,
1:100
), Integrin β3 (
Thermo Fisher Scientific
, USA, cat #
(10)
Deciphering the single-cell transcriptome network in keloids with intra-lesional injection of triamcinolone acetonide combined with 5-fluorouracil
Frontiers in Immunology
May 19, 2023
Yijun Xia, Youbin Wang, Yan Hao, Mengjie Shan, Hao Liu, Zhengyun Liang, Xinwen Kuang
serum blocked. The blocking solution was removed and anti-human Osteopontin antibody (
Abcam
, Cat#:
ab8448
) and anti-Fibronectin antibody (
Abcam
, Cat#:
ab2413
) were added dropwise and incubated flat in a moist
(11)
An enzyme-free technique enables the isolation of a large number of adipose-derived stem cells at the bedside
Scientific reports
May 17, 2023
Yaylacı S, Kaçaroğlu D, Hürkal Ö, Ulaşlı AM.
No:
Ab36861
) at a
1:200
dilution in 0.1% BSA/PBS and anti-osteopontin antibody (
Abcam
, USA Cat No:
Ab8448
) at a
1:200
dilution in 0.1% BSA/PBS. Then to detect primary antibodies
goat anti-rabbit IgG H&L Alexa Fluor 488
(12)
Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regeneration
Stem cell research & therapy
May 15, 2023
Wang Z, Chen C, Zhang J, He J, Zhang L, Wu J, Tian Z.
UK), TGFβR1 (
ab31013
;
Abcam
),
anti-DSPP
(
sc-73632
; Santa Cruz), anti-
DMP1
(
ab103203
;
Abcam
), anti-OPN (
ab8448
;
Abcam
), anti-OSX (
ab13418
;
Abcam
), anti-BMP2 (
ab14933
;
Abcam
), anti-
BMPR1A
(
ab264043
;
Abcam
), anti-p-SMAD1/5
(13)
Multi-leveled Nanosilicate Implants Can Facilitate Near-Perfect Bone Healing
ACS Applied Materials & Interfaces
May 3, 2023
Mozhgan Keshavarz, Parvin Alizadeh, Firoz Babu Kadumudi, Gorka Orive, Akhilesh K. Gaharwar, Miguel Castilho, Nasim Golafshan, Alireza Dolatshahi-Pirouz
were performed by incubating the scaffolds containing fixed cells with primary antibodies, anti-OPN (
ab8448
,
1:100
;
Abcam
), and anti-OCN (
ab13418
,
1:100
;
Abcam
) diluted in PBS containing overnight at 4 °C. After
(14)
Biopotentials of Collagen Scaffold Impregnated with Plant-Cell-Derived Epidermal Growth Factor in Defective Bone Healing
Materials (Basel)
April 24, 2023
Sher Bahadur Poudel, Govinda Bhattarai, Tae-Ho Kwon, Jeong-Chae Lee
USA). Antibodies specific to runt-related transcription factor-2 (Runx2;
ab23981
) and osteopontin (OPN;
ab8448
) were obtained from
Abcam
(Cambridge, UK), while anti-osteocalcin (OCN;
BS7961
) was from
Bioworld Technology
(15)
Circ_0022920 Maintains the Contractile Phenotype of Human Aortic Vascular Smooth Muscle Cells Via Sponging microRNA‐650 and Promoting Transforming Growth Factor Beta Receptor 1 Expression in Angiotensin II‐Induced Models for Aortic Dissection
Journal of the American Heart Association
April 4, 2023
Mao‐Xun Huang, Hu‐Lin Piao, Yong Wang, Zhi‐Cheng Zhu, Ri‐Hao Xu, Tian‐Ce Wang, Dan Li, Ke‐Xiang Liu
antibodies against
human
α‐SMA (
ab5694
,
1:1000
;
Abcam
), SM22α (
ab14106
,
1:1000
;
Abcam
), osteopontin (
ab8448
,
1:1000
,
Abcam
),
MYH11
(
ab133567
,
1:500
,
Abcam
), TGFβR1 (
3712
,
1:1000
,
Cell Signaling Technology
), and
(16)
Cancer‐associated fibroblast‐derived secreted phosphoprotein 1 contributes to resistance of hepatocellular carcinoma to sorafenib and lenvatinib
Cancer Communications
April 1, 2023
Jung Woo Eun, Jung Hwan Yoon, Hye Ri Ahn, Seokhwi Kim, Young Bae Kim, Su Bin Lim, Won Park, Tae Wook Kang, Geum Ok Baek, Moon Gyeong Yoon,
et al.
ab7817
,
Abcam
, Cambridge, UK) and rabbit secreted phosphoprotein 1 (
SPP1
) primary antibodies (
1:100
,
ab8448
,
Abcam
). Briefly, HCC sections were deparaffinized with xylene, rehydrated, unmasked in sodium citrate
(17)
miR-126 mitigates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting the ERK1/2 and Bcl-2 pathways
Acta Biochimica et Biophysica Sinica
March 25, 2023
Ying Zhang, Yiping Dong, Qiushi Wei, Zhikun Zhuang, Youwen Liu, Qiang Yuan, Wei He, Zhenhao Jing, Jitian Li, Peifeng Li,
et al.
antibodies used are listed as follows: anti-OCN (
ab93876
,
1:2000
;
Abcam
, Cambridge, UK), anti-OPN (
ab8448
1:2000
;
Abcam
), anti-
RUNX2
(
ab192256
,
1:500
,
Abcam
),
anti-ALP
(
ab67228
,
1:2000
;
Abcam
), anti-BSP (
ab52128
,
(18)
Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis
International Journal of Molecular Sciences
March 22, 2023
Lu Feng, Zhengmeng Yang, Nan Hou, Ming Wang, Xuan Lu, Yucong Li, Haixing Wang, Yaofeng Wang, Shanshan Bai, Xiaoting Zhang,
et al.
For immunohistochemistry
, primary rabbit anti-OCN (
1:100
,
ab93876
,
Abcam
, Waltham, MA, USA) and rabbit anti-OPN (
1:200
,
ab8448
,
Abcam
, USA) antibodies were used to incubate tissue overnight at 4 °C. After that, the tissue was conjugated
(19)
Insights into pulmonary phosphate homeostasis and osteoclastogenesis emerge from the study of pulmonary alveolar microlithiasis
Nature Communications
March 2, 2023
Yasuaki Uehara, Yusuke Tanaka, Shuyang Zhao, Nikolaos M. Nikolaidis, Lori B. Pitstick, Huixing Wu, Jane J. Yu, Erik Zhang, Yoshihiro Hasegawa, John G. Noel,
et al.
with citrate buffer, blocked with normal serum, and probed with specific primary antibodies anti-OPN (
ab8448
, polyclonal,
1:7500
) and anti-cathepsin K (
ab19027
, polyclonal,
1:750
) obtained from
Abcam
(Cambridge,
(20)
Extended preconditioning on soft matrices directs human mesenchymal stem cell fate via YAP transcriptional activity and chromatin organization
APL Bioengineering
March 1, 2023
Yufei Ma, Xu Zhang, Shaoxin Tang, Li Xue, Jing Wang, Xiaohui Zhang
were incubated with anti-RUNX2 antibody (
1:100
,
Abcam
,
ab76956
) and anti-OPN antibody (
1:100
,
Abcam
,
ab8448
) at 4 °C overnight, respectively. Goat anti-mouse IgG (Alexa Fluor® 488) (
1:1000
, Abcam,
ab150113
) and
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Anti-Osteopontin antibody from Abcam
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