Anti-Ubiquitin antibody [Ubi-1] ab7254 from Abcam

Product Specs
ItemAnti-Ubiquitin antibody [Ubi-1]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab7254
Antibody TypePrimary
Quantity50 UL
ClonalityMonoclonal
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneUbi-1
HostMouse, Nude mouse
IsotypeIgG1
Conjugate/TagUnconjugated
Concentration5 mg/ml
Gene NameUBC
ReactivityHuman, Mouse
TargetUbiquitin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro modelsCurrent Research in NeurobiologyJuly 30, 2023Mira Seidel, Sandeep Rajkumar, Christina Steffke, Vivien Noeth, Shreya Agarwal, Kevin Roger, Joanna Lipecka, Albert Ludolph, Chiara Ida Guerrera, Tobias Boeckers, et al.anti-LAMP1 (Invitrogen, MA1-164), anti-cleaved caspase 3 (Cell Signaling, 9661), anti-ubiquitin (Abcam, ab7254), anti-C9orf72 (Genetex, GTX632041, anti-β-actin (Sigma-Aldrich, A5441), anti-RagC (Cell Signaling,

(2) RP11-40C6.2 Inactivates Hippo Signaling by Attenuating YAP1 Ubiquitylation in Hepatitis B Virus-associated Hepatocellular CarcinomaJournal of Clinical and Translational HepatologyApril 28, 2023Han Zhuo, Chen Wu, Junwei Tang, Feihong Zhang, Zhenggang Xu, Dongwei Sun, Yue Teng, Zhongming Tancontaining 10 mM N-Ethylmaleimide. YAP1 was immunoprecipitated and anti-ubiquitin antibody (1:1,000, ab7254; Abcam, Cambridge, UK) was used to detect ubiquitination. To examine the interaction of TAZ with HBx

(3) UBE2T promotes glioblastoma malignancy through ubiquitination-mediated degradation of RPL6Cancer scienceFebruary 1, 2023Tao X, Wu X, Zhou P, Yu X, Zhao C, Peng X, Zhang K, Shen L, Peng J, Yang L.Anti RPL30 (A13690), anti Actin (AC026), and anti Flag (F1804) were purchased from Sigma. Anti Ub (ab7254) was purchased from Abcam. 2.6 CCK8 assay According to the instructions, the cell viability was measured

(4) An antisteatosis response regulated by oleic acid through lipid droplet–mediated ERAD enhancementScience AdvancesJanuary 6, 2023Jorge Iván Castillo-Quan, Michael J. Steinbaugh, Laura Paulette Fernández-Cárdenas, Nancy K. Pohl, Ziyun Wu, Feimei Zhu, Natalie Moroz, Veronica Teixeira, Monet S. Bland, Nicolas J. Lehrbach, et al.velocity, and loaded into NuPAGE Novex bis-tris 10% gels. Antibodies used were ubiquitin (1:500; Abcam, ab7254), glyceraldehyde-3-phosphate dehydrogenase (1:500; Bethyl Laboratories, A303-878A), and GFP (1:500;

(5) Melatonin reduces the endoplasmic reticulum stress and polyubiquitinated protein accumulation induced by repeated anesthesia exposure in Caenorhabditis elegansScientific ReportsDecember 1, 2022Hyun-Jung Shin, Bon-Wook Koo, Jiwon Yoon, Heeyeon Kim, Sang-Hwan Do, Hyo-Seok Na20, pH 7.5), and incubated for 1 h at 20 °C with primary anti-ubiquitin antibody (1:1000; ABCAM Cat# ab7254, RRID:AB_305802) and anti-actin antibody (1:5000; ABCAM Cat# ab14128, RRID:AB_300931), followed by horseradish

(6) Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferationPlant CommunicationsNovember 14, 2022Yangyi Zheng, Sensen Zhang, Yanqiu Luo, Fuquan Li, Jiantao Tan, Bin Wang, Zhe Zhao, Huifang Lin, Tingting Zhang, Jianhong Liu, et al.separated by SDS–PAGE, blotted, and analyzed via western blotting with antibodies against Ub (mouse, Abcam ab7254, 1:2000 dilution), MBP, H2Aub1 (rabbit, Cell Signaling Technology 8240T, 1:1000 dilution), and H2Bub1

(7) Stress-inducible phosphoprotein 1 (HOP/STI1/STIP1) regulates the accumulation and toxicity of Î±-synuclein in vivoActa NeuropathologicaNovember 1, 2022Rachel E. Lackie, Aline S. de Miranda, Mei Peng Lim, Vladislav Novikov, Nimrod Madrer, Nadun C. Karunatilleke, Benjamin S. Rutledge, Stephanie Tullo, Anne Brickenden, Matthew E. R. Maitland, et al.RRID:AB_2728613), anti-GFAP (1:400, Cat# ab4674, Abcam, RRID:AB_304558), anti-ubiquitin (1:50, Cat# ab7254, Abcam, RRID:AB_305802), anti-Hsp90 (1:50, Cat# 4877, Cell Signaling Technology, RRID:AB_2233307), and

(8) ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinomaJournal of Cellular and Molecular MedicineOctober 1, 2022Congcong Zhang, Jiangtao Cui, Leiqun Cao, Xiaoting Tian, Yayou Miao, Yikun Wang, Shiyu Qiu, Wanxin Guo, Lifang Ma, Jinjing Xia, et al.anti‐glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH, Abcam, #ab9485 and #ab8245), anti‐Ub (Abcam, #ab134953 and #ab7254), anti‐FLAG (CST, Boston, MA, USA #14793 and #8146), anti‐PSMB5 (Abcam, #ab3330), anti‐aldolase (ALDO,

(9) Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC mouse expressing misfolded HGSNATThe Journal of experimental medicineAugust 1, 2022Pan X, Taherzadeh M, Bose P, Heon-Roberts R, Nguyen ALA, Xu T, Pará C, Yamanaka Y, Priestman DA, Platt FM, et al.(ab104898) PSD-95,Mouse anti-mouse,"1:1,000 (cells) and 1:200 (tissues)",Abcam (ab99009) Ubiquitin,Mouse,1:200,Abcam (ab7254) β-Amyloid (D54D2),Rabbit anti-mouse,1:200,Cell Signaling (8243S) SCMAS,Rabbit anti-mouse,1:200,Abcam

(10) YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcriptionCell death discoveryFebruary 11, 2022Xue X, Tian X, Zhang C, Miao Y, Wang Y, Peng Y, Qiu S, Wang H, Cui J, Cao L, et al.#ab52771 and Santa Cruz, #sc-101199), anti-GAPDH (CST, #5174 and #51332), anti-Ub (Abcam, #ab7780 and #ab7254), anti-PSMB5 (Abcam, #ab167341), anti-βTrCP (Abcam, #ab71753 and #ab233638), anti-YAPO241 (developed

(11) The autophagic protein p62 is a target of reactive aldehydes in human and murine cholestatic liver diseasePloS oneJanuary 1, 2022Shearn CT, Anderson AL, Devereux MW, Orlicky DJ, Michel C, Petersen DR, Miller CG, Harpavat S, Schmidt EE, Sokol RJ.Abcam), phospho Serine 129 gamma H2Ax (9718, Cell Signaling Technologies, Danvers, MA), Ubiquitin (ab7254, Abcam), Heme Oxygenase 1 (SPA-895, Enzo Lifesciences, Framingham, NY), p62 (NBP1-48320, NBP1-48320AF594,

(12) HIF-1/2α-Activated RNF146 Enhances the Proliferation and Glycolysis of Hepatocellular Carcinoma Cells via the PTEN/AKT/mTOR PathwayFrontiers in cell and developmental biologyJanuary 1, 2022Shen G, Wang H, Zhu N, Lu Q, Liu J, Xu Q, Huang D.Proteintech), p-mTOR (Ser2448, 67778-1-Ig, Proteintech), mTOR (66888-1-Ig, Proteintech), Ubiquitin (ab7254, Abcam). 2.6 Immunohistochemistry The embedded-paraffin tissues were sectioned with a thickness of

(13) Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosisActa neuropathologicaDecember 1, 2021Diaz-Garcia S, Ko VI, Vazquez-Sanchez S, Chia R, Arogundade OA, Rodriguez MJ, Traynor BJ, Cleveland D, Ravits J.Proteintech), ELAVL4 (#24992-1-AP Proteintech), ELAVL2 (#67097-1-AP Proteintech), and Ubiquitin (Ubi-1 ab7254, Abcam). The antibodies for ELAVL1 and ELAVL3 were previously validated by KO studies [35, 56, 59, 62].

(14) A novel dephosphorylation targeting chimera selectively promoting tau removal in tauopathiesSignal Transduction and Targeted TherapyDecember 1, 2021Jie Zheng et al.1:1000), PP2A-Bα (4953, Cell signaling, 1:1000), Phospho-Ser/Thr (ab17464, Abcam1:1000), Ubiquitin (ab7254, Abcam, 1:1000), MAP2 (AB5622, Millipore, 1:2000), hTau-N368 (a generous gift from Prof. Keqiang Ye,

(15) SHARPIN regulates the development of clear cell renal cell carcinoma by promoting von Hippel-Lindau protein ubiquitination and degradationCancer scienceOctober 1, 2021Yin R, Liu S.anti‐SHARPIN (1:1000, 14626‐1‐AP; Proteintech), anti‐HIF‐2α (1:1000, ab243861; Abcam), anti‐ubiquitin (1:1000, ab7254; Abcam), anti‐pVHL (1:500, sc‐17780; Santa Cruz Biotechnology), anti‐HA (1:1000, 3724; Cell Signaling

(16) Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionCell death and differentiationOctober 1, 2021Liu Y, Mukherjee R, Bonn F, Colby T, Matic I, Glogger M, Heilemann M, Dikic I.against GST (cell signaling technology), His (Roche), GRASP55 (Proteintech), GRASP65 (Sino biotech.), Ub (ab7254 from Abcam, 39365 from Cell signaling technology). To assess the PR-ubiquitination of GRASP55 and GRASP65

(17) Developmental Consequences of Defective ATG7-Mediated Autophagy in HumansNew England Journal of MedicineJune 24, 2021Jack J. Collier et al.following primary and secondary antibodies: P62 (1 µg/ml, Abcam ab109012), Ubiquitin (1:500, Abcam ab7254), goat anti-mouse IgG1 AlexaFluor 488 (1:200, Invitrogen A-21121) and goat anti-rabbit IgG 12 Collier

(18) FBXO32 links ubiquitination to epigenetic reprograming of melanoma cellsCell Death & DifferentiationJune 1, 2021Nadia Habel et al.and then probed with antibodies to FBXO32 (Abcam, Ab168372), MITF (Abcam, Ab12039), ubiquitin (Abcam, ab7254), CDK6 (DCS83, Cell Signaling Technology), SMAD7 (Santa Cruz Biotechnology, sc-11392), BRG1 (Abcam,

(19) UXT chaperone prevents proteotoxicity by acting as an autophagy adaptor for p62-dependent aggrephagyNature communicationsMarch 29, 2021Yoon MJ, Choi B, Kim EJ, Ohk J, Yang C, Choi YG, Lee J, Kang C, Song HK, Kim YK, et al.immunoprecipitation (IP), and immunohistochemistry (IHC): mouse monoclonal anti-ubiquitin (1:200 (ICC), Abcam, ab7254), rabbit monoclonal anti-ubiquitin (1:200 (ICC), Abcam, ab134953), mouse monoclonal anti-p62 (1:2000

(20) Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infectionProceedings of the National Academy of Sciences of the United States of AmericaMarch 9, 2021Lin Y, Hu Q, Zhou J, Yin W, Yao D, Shao Y, Zhao Y, Guo B, Xia Y, Chen Q, et al.were determined by Western blot with anti-RFP (Cat. No. 6G6, ChromoTek) and anti-ubiquitin (Cat. No. ab7254, Abcam). Protein Expression and Purification. The GmPUB13 U-box and Avr1d were both cloned into the

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Anti-Ubiquitin antibody [Ubi-1] from Abcam

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Anti-Ubiquitin antibody [Ubi-1] from Abcam

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