Alexa Fluor(R) 488 anti-mouse CD3epsilon 100321 from BioLegend

Product Specs
ItemAlexa Fluor(R) 488 anti-mouse CD3epsilon
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100321
NCBI Gene AliasesCD3, CD3epsilon, T3e
Quantity100 µg
ReactivityMouse
IsotypeIgG
ApplicationsFlow Cytometry (FC)
Conjugate/TagAlexa Fluor® 488
HostArmenian Hamster
Clone145-2C11
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, epsilon polypeptide
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenH-2K^b-specific mouse cytotoxic T lymphocyte clone BM10-37
ClonalityMonoclonal
TargetCD3epsilon

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

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Website: biolegend.com

(1) Selective activation and expansion of regulatory T cells using lipid encapsulated mRNA encoding a long-acting IL-2 muteinNature CommunicationsDecember 1, 2022Seymour de Picciotto, Nicholas DeVita, Chiaowen Joyce Hsiao, Christopher Honan, Sze-Wah Tse, Mychael Nguyen, Joseph D. Ferrari, Wei Zheng, Brian T. Wipke, Eric HuangeFluor780 (ThermoFischer L34961) at 1:100 dilution, and surface antibodies CD3e AF488 (1:400, Biolegend 100321), CD4 BV510 (1:1600, Biolegend 100449), CD8a BV570 (1:400, Biolegend 100740), NK1.1 AF700 (1:800, Biolegend

(2) Do more with Less: Improving High Parameter Cytometry Through Overnight StainingCurr ProtocNovember 1, 2022Whyte CE, Tumes DJ, Liston A, Burton OT.Violet 421,GK1.5,1:1000,3,BioLegend,100443 Anti‐mouse CD3 Alexa Fluor 488,145‐2C11,1:1000,3,BioLegend,100321 Anti‐mouse CCR7 APC‐Fire 810,4B12,"1:50, 1:200",4,BioLegend,120135 Anti‐mouse CD3 Spark Blue 550,17A2,"1:500,

(3) Intratumoral Treatment with 5-Androstene-3Î², 17Î±-Diol Reduces Tumor Size and Lung Metastasis in a Triple-Negative Experimental Model of Breast CancerInternational Journal of Molecular SciencesOctober 8, 2022RocÃo Alejandra Ruiz Manzano, Karen Elizabeth Nava-Castro, Margarita Isabel Palacios-Arreola, RosalÃa HernÃ¡ndez-Cervantes, VÃctor Hugo Del RÃo-Araiza, Mariana Segovia-Mendoza, Armando PÃ©rez-Torres, Manuel IvÃ¡n GirÃ³n-PÃ©rez, Jorge Morales-Montorwere stained with the following panels. For T lymphocyte: AlexaFluor 488-conjugated anti-CD3ε (Cat# 100321, Clone 145-2C11, RRID:AB_389301) 1:100, PE-conjugated anti-CD4 (Cat# 100407, Clone GK1.5, RRID:AB_2075573)

(4) Humanized Mouse Model as a Novel Approach in the Assessment of Human Allogeneic Responses in Organ TransplantationFrontiers in ImmunologyJune 11, 2021Ashwin Ajith, Laura L. Mulloy, Md. Abu Musa, Valia Bravo-Egana, Daniel David Horuzsko, Imran Gani, Anatolij Horuzsko1:300); Anti-human/mouse antibody Granzyme B (515403, clone GB11, 1:300); Anti-mouse antibodies: CD3 (100321, clone 145-2C11, 1:300), CD4 (100407, clone GK1.5, 1:200), CD8 (100713, clone 53-6.7, 1:300), and CD25

(5) Itaconate confers tolerance to late NLRP3 inflammasome activationCell reportsMarch 9, 2021Bambouskova M, Potuckova L, Paulenda T, Kerndl M, Mogilenko DA, Lizotte K, Swain A, Hayes S, Sheldon RD, Kim H, et al.monoclonal Ab",Biolegend,Cat# 653904; RRID:AB_2564508 "CD3, AF488, hamster monoclonal Ab",Biolegend,Cat# 100321; RRID:AB_389300 "CD4, BV711, rat monoclonal Ab",Biolegend,Cat# 100549; RRID:AB_11219396 "CD8, BV421,

(6) Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK⁺ CD8⁺ T Cells as Conserved Hallmark of InflammagingImmunityJanuary 12, 2021Mogilenko DA, Shpynov O, Andhey PS, Arthur L, Swain A, Esaulova E, Brioschi S, Shchukina I, Kerndl M, Bambouskova M, et al.

(7) Digenic mutations in ALDH2 and ADH5 impair formaldehyde clearance and cause a multisystem disorder, AMeD syndromeScience AdvancesDecember 18, 2020Yasuyoshi Oka et al.421, no. 135313, BioLegend), CD34 (APC/Fire 750, no. 135014, BioLegend), CD3 (Alexa Fluor 488, no. 100321, BioLegend), CD19 (APC, no. 152410, BioLegend), CD4 (PE, no. 130310, BioLegend), and CD8a (APC, no.

(8) SOX2 promotes resistance of melanoma with PD-L1 high expression to T-cell-mediated cytotoxicity that can be reversed by SAHAJournal for immunotherapy of cancerNovember 1, 2020Wu R, Wang C, Li Z, Xiao J, Li C, Wang X, Kong P, Cao J, Huang F, Li Z, et al.Cell Signaling Technology (Danvers, MA, USA). Flow cytometry antibodies: Alexa Fluor 488 antimouse CD3ε (No. 100321), Alexa Fluor 647 antihuman/mouse GranzymeB (No. 515406) and PerCP/Cyanine5.5 antimouse IFNγ (No. 505821)

(9) Diet Diurnally Regulates Small Intestinal Microbiome-Epithelial-Immune Homeostasis and EnteritisCellSeptember 17, 2020Tuganbaev T, Mor U, Bashiardes S, Liwinski T, Nobs SP, Leshem A, Dori-Bachash M, Thaiss CA, Pinker EY, Ratiner K, et al.

(10) High Dimensional Single-Cell Analysis Reveals iNKT Cell Developmental Trajectories and Effector Fate DecisionCell reportsSeptember 8, 2020Baranek T, Lebrigand K, de Amat Herbozo C, Gonzalez L, Bogard G, Dietrich C, Magnone V, Boisseau C, Jouan Y, Trottein F, et al.

(11) Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor GrowthCell reportsMay 19, 2020Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, Kar G, Vento-Tormo R, Hagai T, Chen X, et al.Syrian Hamster PDPN APC,Biolegend,"Cat# 127410, RRID:AB_10613649" Armenian Hamster CD3e 488,Biolegend,"Cat# 100321, RRID:AB_389300" Armenian Hamster CD3e PE,Biolegend,"Cat# 100308, RRID:AB_312673" Mouse NK1.1 PE,Biolegend,"Cat#

(12) Potential Novel Risk Factor for Breast Cancer: Toxocara canis Infection Increases Tumor Size Due to Modulation of the Tumor Immune MicroenvironmentFrontiers in oncologyJanuary 1, 2020Ruiz-Manzano RA, Palacios-Arreola MI, Hernández-Cervantes R, Del Río-Araiza VH, Nava-Castro KE, Ostoa-Saloma P, Muñoz-Cruz S, Morales-Montor J.were stained with the following panels. For T lymphocyte: AlexaFluor®488-conjugated anti-CD3ε (Cat# 100321, Clone 145-2C11, RRID:AB_389301) 1:100, PE-conjugated anti-CD4 (Cat# 100407, Clone GK1.5, RRID:AB_2075573)

(13) Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem CellsCell reportsDecember 17, 2019Chen C, Yu W, Tober J, Gao P, He B, Lee K, Trieu T, Blobel GA, Speck NA, Tan K.A2F10),Biolegend,Cat# 135313; RRID:AB_2562338 AF488 anti-mouse CD3ε (Clone 145-2C11),Biolegend,Cat# 100321; RRID:AB_389300 AF488 anti-mouse Ter119 (Clone TER-119),Biolegend,Cat# 116215; RRID:AB_493402 AF488

(14) Extracellular traps and PAD4 released by macrophages induce citrullination and auto-antibody production in autoimmune arthritisJournal of AutoimmunityDecember 1, 2019Mohey Eldin M. El Shikh, Riham El Sayed, Alessandra Nerviani, Katriona Goldmann, Christopher Robert John, Rebecca Hands, Liliane Fossati-Jimack, Myles J. Lewis, Costantino Pitzaliswith TruStainFcX (Biolegend, 101320), a panel of CD19 PE (Biolegend, 115508), CD3 AF488 (Biolegend, 100321), CD11b BV605 (Biolegend, , 101257) and F4/80 PE-Cy7 (Biolegend, 123114) was used and the plots were

(15) HLA-G dimer targets Granzyme B pathway to prolong human renal allograft survivalFASEB journal : official publication of the Federation of American Societies for Experimental BiologyApril 1, 2019Ajith A, Portik-Dobos V, Nguyen-Lefebvre AT, Callaway C, Horuzsko DD, Kapoor R, Zayas C, Maenaka K, Mulloy LL, Horuzsko A.

(16) Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to TissuesImmunityDecember 18, 2018He W, Holtkamp S, Hergenhan SM, Kraus K, de Juan A, Weber J, Bradfield P, Grenier JMP, Pelletier J, Druzd D, et al.17A2",Biolegend,"Cat# 100246, RRID: AB_2565883" "Anti-mouse CD3ε, Alexa Fluor® 488, clone 145-2C11",Biolegend,"Cat# 100321, RRID: AB_389300" "Anti-mouse CD4, Brilliant Violet 570, clone RM4-5",Biolegend,"Cat# 100542, RRID:

(17) Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous proteinJCI insightAugust 23, 2018Burman A, Kropski JA, Calvi CL, Serezani AP, Pascoalino BD, Han W, Sherrill T, Gleaves L, Lawson WE, Young LR, et al.eBioscience/Thermo Fisher Scientific), CD19-PE (12-0193-81, eBioscience/Thermo Fisher Scientific), and CD3-Alexa Fluor 488 (100321, BioLegend). Samples were acquired on an LSRII machine (BD Biosciences), and data were analyzed using

(18) Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behaviorNature immunologyDecember 1, 2017Miyajima M, Zhang B, Sugiura Y, Sonomura K, Guerrini MM, Tsutsui Y, Maruya M, Vogelzang A, Chamoto K, Honda K, et al.

(19) Filamin A Regulates Neutrophil Adhesion, Production of Reactive Oxygen Species, and Neutrophil Extracellular Trap ReleaseJournal of immunology (Baltimore, Md. : 1950)November 15, 2017Uotila LM, Guenther C, Savinko T, Lehti TA, Fagerholm SC.

(20) Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfacesJCI insightAugust 17, 2017Azzam KM, Madenspacher JH, Cain DW, Lai L, Gowdy KM, Rai P, Janardhan K, Clayton N, Cunningham W, Jensen H, et al.IgM APC (Biolegend, 406515); CD43 PerCP-Cy5.5 clone S7 (BD Biosciences, 562865); CD3-AF488 (Biolegend, 100321); IgM-PE (Biolegend, 406508); CD43 clone 1B11 APC/Cy7 (Biolegend, 121220); GL7-FITC (Biolegend, 144603);

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(1)

1/7/2019Successful Detection of Tumour Infiltrating T cells Jamie WallisThis antibody was used to quantify T cell infiltration into tumours. Staining was clear but required a lot of antibody.

International Journal of Molecular Sciences

Frontiers in oncology

International Journal of Molecular Sciences

Frontiers in oncology

International Journal of Molecular Sciences

Alexa Fluor(R) 488 anti-mouse CD3epsilon from BioLegend

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Alexa Fluor(R) 488 anti-mouse CD3epsilon from BioLegend

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