Miltenyi Biotec's REAlease® Releasable Antibodies is a Yeast monoclonal antibody. It reacts with Human, and Mouse. This antibody has been shown to work in applications such as: Flow Cytometry.
Description
REAlease® Fluorochrome Technology
REAlease® Releasable Antibodies allow to obtain label-free cells after flow cytometry-based cell sorting in just one step.
The technology relies on recombinantly engineered antibody fragments that are, unlike conventional antibodies, characterized by low epitope binding affinities. A novel covalent conjugation approach allows for multimerization and fluorescent labeling of these antibody fragments. They form the so called REAlease Fluorochrome Complex which facilitates high-avidity cell binding that is comparable to conventional fluorochrome conjugates.
This novel and innovative conjugation chemistry allows for fast removal of the REAlease Fluorochrome Complex from the cell surface by adding the REAlease Release Reagent after cell sorting. This leads to disruption of the complex and thereby results in monomerization of the antibody fragments.
Due to their engineered low binding affinity, they spontaneously dissociate from the cell surface. As a result, label-free cells can be obtained after cell sorting providing maximal flexibility for a wide range of applications.
The benefits
- Epitope of a marker becomes available again for downstream applications that require free epitopes (e.g. cell culture experiments and cell analysis)
- Fluorescence channel becomes available again for relabeling (e.g. microscopy applications)
- Sequential flow sorting cycles for the isolation of specific cell subsets