Fig 1: Validation of the identified proteins in dendritic filopodia. (A,B) Localization of the identified proteins in dendritic filopodia (A1–A21) and phagocytic cups (B1–B21) at 14 DIV hippocampal neurons. The cultured neurons were immunostained with anti-TLCN antibody and specific antibodies against Gao (A1,B1), Na+/K+ ATPase a3 (A2,B2), EFA6D (A3,B3), spectrin (A4,B4), septin7 (A5,B5), myosin VA (A6,B6), Gaq (A7,B7), Gß2 (A8,B8), eEF1? (A9,B9), ribosomal protein S16 (A10,B10), CaMKIIa (A11,B11), CD98hc (A12,B12), EFA6C (A13,B13), BAIAP2L1 (A14,B14), PLCß3 (A15,B15), MRCKa (A16,B16), NR3A/B (GluN3A/B) (A17,B17), SAP97 (SLC3A2) (A18,B18), MAP1S (A19,B19), EPS8L1 (A20,B20), and JIP-4 (SPAG9) (A21,B21). In merged images, the identified proteins and TLCN are shown in magenta and green, respectively. Single plane of images focused on dendritic filopodia (A1–A21) and center of microbeads (B1–B21) were acquired using a confocal microscopy. Scale bars, 5 µm in (A14,A21,B14,B21).