Anti-CD133 antibody [EPR16508] ab222782 from Abcam

Product Specs
ItemAnti-CD133 antibody [EPR16508]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222782
Quantity10 UL, 40 UL, 100 UL
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
NCBI Gene AliasesAC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
ClonalityMonoclonal
CloneEPR16508
Research AreaNeuroscience
Gene NamePROM1
ReactivityHuman
TargetCD133

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(1) E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathwayFrontiers in OncologyJune 29, 2023Qingkun Gao, Ke An, Zhe Lv, Yanzhao Wang, Changmin Ding, Wensheng Huang(ab152126; dilution 1:500; rabbit; Abcam, Shanghai, China), A2B5 (ab53521; dilution 1 µg/ml; mouse), CD133 (ab222782; dilution 1:2,000; rabbit), Nestin (ab105389; dilution 1:100; rabbit), Sox2 (ab97959; dilution 1 µg/ml;

(2) Dual role of ANGPTL8 in promoting tumor cell proliferation and immune escape during hepatocarcinogenesisOncogenesisMay 15, 2023Gao Y, Yuan Y, Wen S, Chen Y, Zhang Z, Feng Y, Jiang B, Ma S, Hu R, Fang C, et al.19]. Western blotting The membranes were incubated with antibodies against ANGPTL8 (ab180915, Abcam), CD133 (ab222782, Abcam), Beclin-1 (3495S, CST), ATG5 (AF2269, Beyotime), P62 (5114s, CST), LC3I/II (14600-1-AP, Proteintech),

(3) UVRAG Promotes Tumor Progression through Regulating SP1 in Colorectal CancerCancers (Basel)April 27, 2023Mengyuan Shi, Guo An, Nan Chen, Jinying Jia, Xinxin Cui, Tiancheng Zhan, Dengbo Jidiluted by 1:500, anti-ABCG2 (#AP1490b-ev; Abgent, San Diego, CA, USA) diluted by 1:1000, anti-CD133 (#ab222782; Abcam) diluted by 1:2000, and anti-BMI1 (#ab126783; Abcam) diluted by 1:1000. After washing with Tris-buffered

(4) SCF‐FBXL8 contributes to liver metastasis and stem‐cell‐like features in colorectal cancer cells by mediating ubiquitination and degradation of TP53Clinical and Translational MedicineMarch 1, 2023Jing Yao, Xin‐Ping Wang, Jun Yang, Zhe Yang, Zheng‐Yun Zhang(Santa Cruz Biotechnology, California, USA, cat sc‐390582), p53 (Abcam, Cambridge, UK, cat ab26), CD133 (Abcam, cat ab222782) and Ki67 (Abcam, cat ab15580). For staining, scores of FBXL8 and p53 in tissues were determined as

(5) CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinomaCell death & diseaseFebruary 11, 2023Shen Y, Chen Y, Lin Y, Li Y, Liu P, Zhang B, Wang Y, Chan KC, Mak NK, Kahn M, et al.cat. no. Ab92494), anti-CD44 (1:4000; Abcam, cat. no. ab189524), anti-CD133 (1:1000; Abcam, cat. no. ab222782), anti-Notch1 (1:150; Abcam, cat. no. ab52627), anti-EZH2 (1:200; Cell Signaling Technology, cat. no.

(6) m6A-modified circFNDC3B inhibits colorectal cancer stemness and metastasis via RNF41-dependent ASB6 degradationCell Death & DiseaseNovember 29, 2022Wei Zeng, Jin-Feng Zhu, Jian Guo, Gen-Jie Huang, Li-Sha Ai, Yu Zeng, Wang-Jun Liao(1:1000, ab181557, Abcam), Nanog (1:3000, ab109250, Abcam), SOX2 (1:1000, ab92494, Abcam), CD133 (1:2000, ab222782, Abcam), or anti-GAPDH antibody (1:1000, ab8245, Abcam) at 4 °C overnight. This is followed by the incubation

(7) Circular RNA circPTPRF promotes the progression of GBM via sponging miR-1208 to up-regulate YY1Cancer Cell InternationalNovember 17, 2022Jiang Zhou, Chengbin Wang, Yingliang Liu, Daming Cui, Zhenlin Wang, Yang Jiang, Liang Gao#ab7751, Abcam), GFAP (1:1000, #ab4674, Abcam), nestin+ (1:500, #ab18102, Abcam) and CD133 (1:1000, #ab222782, Abcam). DAPI (#D9542, Sigma) was used for staining the nuclei. Use a laser scanning confocal microscope

(8) The long noncoding RNA TINCR promotes self-renewal of human liver cancer stem cells through autophagy activationCell Death DisNovember 16, 2022Jing Shi, Cao Guo, Yang Li, Junli Ma(1:1000 dilution; ab108327, Abcam), PTBP1 (1:5000 dilution, ab133734, Abcam), CD133 (1:1000 dilution, ab222782, Abcam), SOX2 (1:1000 dilution, ab171380, Abcam), TOM20 (1:1000 dilution, #42406 S, CST), Notch1 (1:1000

(9) HSP90 facilitates stemness and enhances glycolysis in glioma cellsBMC NeurologyNovember 11, 2022Xun Kang, Jing Chen, Jian-feng Houprobed with anti-HSP90 (1:1000, ab13492, Abcam, Shanghai, China), A2B5 (1:1000, ab53521), CD133 (1:1000, ab222782), Nestin (1:1000, ab105389), Sox2 (1:1000, ab97959) and GAPDH (1:1000, ab8245) primary antibodies. Membranes

(10) Connexin32 regulates expansion of liver cancer stem cells via the PI3K/Akt signaling pathwayOncology reportsSeptember 1, 2022Li H, Wang B, Qi B, Jiang G, Qin M, Yu M.code ab81283), total Akt (product code ab8805), EpCAM (product code ab223582), CD133 (product code ab222782), Nanog (product code ab14959), Oct4 (product code ab181557), c-Myc (product code ab32072), β-actin

(11) CD133, but Not CD44, May Serve as a Novel Biomarker for Differential Diagnosis Between Basal Cell Carcinoma and TrichoblastomasClinical, Cosmetic and Investigational DermatologyAugust 2, 2022Yalan Bi, Xiaohua Shi, Dian Chen, Yi Zhao(H2O2). The sections were then incubated with rabbit anti-human CD133 monoclonal antibody (cat no. ab222782, 1:000, Abcam) or rabbit anti-human CD44 monoclonal antibody (cat no. ab189524, 1:4000, Abcam) overnight

(12) Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma CellsInternational Journal of Molecular SciencesJune 30, 2022Tung-Yu Tiong, Pei-Wei Weng, Chun-Hua Wang, Syahru Agung Setiawan, Vijesh Kumar Yadav, Narpati Wesa Pikatan, Iat-Hang Fong, Chi-Tai Yeh, Chia-Hung Hsu, Kuang-Tai Kuoab109250, Abcam, Cambridge, MA, USA), OCT4 (Cat. # ab200834, Abcam, Cambridge, MA, USA), CD133 (Cat. # ab222782, Abcam, Cambridge, MA, USA), FASN (Cat. # ab128870, Abcam, Cambridge, MA, USA), β-actin (Cat. # 66009-1-Ig,

(13) Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR ‐493‐3p/ RAB22A axis in non‐small cell lung cancerThoracic CancerFebruary 1, 2022Dongxiao Ding, Feng Yang, Zhongjie Chen, Junjie Ying(ab32124; 1:500; Abcam), OCT4 (ab200834; 1:10000; Abcam), NANOG (ab109250; 1:2 000; Abcam), CD133 (ab222782; 1:2000; Abcam) and anti‐β‐actin (ab8227; 1:1 000; Abcam). Cell transfection RAB22A overexpression

(14) Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78BioengineeredFebruary 1, 2022Yan Zhong, Jian Lanfor IHC, ab15580, Abcam), CD44 (1:500, ab189524, Abcam), CD133 (1:500 for Immunoblot, 1:100 for IHC, ab222782, Abcam), SOX2 (1:500, ab92494, Abcam), ALDH1 (1:1000, ab177463, Abcam), FAK (1:1000, ab40794, Abcam),

(15) Circular RNA circASPM promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-130b-3p/E2F1 axisJournal of CancerJanuary 1, 2022Hou D, Wang Z, Li H, Liu J, Liu Y, Jiang Y, Lou M.followed with FITC- or rhodamine-conjugated secondary antibody. The antibodies against CD133 (1:1000, #ab222782, Abcam), nestin+ (1:500, #ab18102, Abcam), GFAP (1:1000, #ab4674, Abcam) and β III tubulin (1:1000,

(16) Effect of endothelial progenitor cell-derived extracellular vesicles on endothelial cell ferroptosis and atherosclerotic vascular endothelial injuryCell Death DiscoveryDecember 1, 2021Lin Li, Haining Wang, Jing Zhang, Xiao Chen, Zhongwang Zhang, Qiang Liserum, and incubated with the primary antibodies CD34 (ab110643, Abcam Inc., Cambridge, MA, USA), CD133 (ab222782, Abcam), vWF (ab6994, Abcam), and VEGF-2 (ab2349, Abcam) at 4 °C overnight. Then, the cells were added

(17) Effects of CD133 expression on chemotherapy and drug sensitivity of adenoid cystic carcinomaMolecular Medicine ReportsNovember 17, 2021Lian Zhou, Ying Sun, Guo Ye, Yanguang Zhao, Jian Wu1.5 h. PVDF membranes were incubated with primary antibodies, including anti-CD133 (1:1,000; cat. no. ab222782; Abcam), anti-MDR1 (1:1,000; cat. no. ab235954; Abcam), anti-MRP1 (1:1,000; cat. no. ab3368; Abcam)

(18) Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9Molecular Medicine ReportsOctober 11, 2021Zhuanzhuan Zhou, Yanping Lai, Shan Cao, Qifang Zhuo, Huiqin Tang1:1,000), SOX2 (cat. no. ab171380, 1:1,000), CD44 (cat. no. ab189524, 1:1,000) and CD133 (cat. no. ab222782, 1:1,000) were added and incubated overnight at 4°C. All antibodies were purchased from Abcam. HRP-labeled

(19) Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axisBioengineeredSeptember 13, 2021Jiwen Xu, Xiaoyan Liu, Xueting Liu, Yunlai Zhimembranes were blocked with 5% nonfat milk and then incubated with primary antibodies against CD133 (ab222782, 1:2000, Abcam), Nanog (ab109250, 1:1000, Abcam), Oct4 (ab200834, 1:10,000, Abcam), Sox2 (ab92494, 1:1000,

(20) MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2Animal Cells and SystemsJuly 15, 2021Paerhati Shayimu, Aikeremu Yusufu, Aizimaiti Rehemutula, Darebai Redati, Rexida Jiapaer, Rousidan Tuerdiapplied in this were MTBP (Abcam, ab115529), ZEB2 (Abcam, ab138222), CD44 (Abcam, ab51037), CD133 (Abcam, ab222782) and β-actin (Abcam, ab8227). All these primary antibodies were diluted with the BSA (Beyotime, China)

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Oncology Letters

Molecular Medicine Reports

Anti-CD133 antibody [EPR16508] from Abcam

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Anti-CD133 antibody [EPR16508] from Abcam

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