Fig 1: Knockdown of CD133 expression in drug-resistant cell lines enhances cell sensitivity to chemotherapy drugs. (A) RT-qPCR was performed to detect the expression of CD133. RT-qPCR detected the expression of drug-resistant associated genes in (B) KOA-1/5-FU and (C) KOA-1/PYM cells. ***P<0.001. CD, cluster of differentiation; RT-qPCR, reverse transcription-quantitative PCR; 5-FU, 5-fluorouracil; PYM, pingyangmycin; siRNA, small interfering RNA; NC, negative control; MDR1, multidrug resistance protein 1; MRP1, multidrug resistance-associated protein 1.
Fig 2: Cell viability after knockdown of CD133 expression in cells. (A) The expression of CD133 after cell transfection was detected by reverse transcription-quantitative PCR. (B) Cell Counting Kit-8 assay was performed to detect cell viability. ***P<0.001. CD, cluster of differentiation; siRNA, small interfering RNA; NC, negative control; 5-FU, 5-fluorouracil; PYM, pingyangmycin.
Fig 3: Representative imaging of H&E staining and immunohistochemical staining of CD133 and CD44 in BCC and TB cases. H&E staining shows: (A) uniform cell structure and no infiltrative growth pattern, with a pushing edge, minimal cellular pleomorphism, and sparse mitosis in a TB case; (B) infiltrative growth pattern of BCC into the striated muscle, with marked cellular pleomorphism and active mitosis; Immunohistochemical staining of CD133 in TB (C) and BCC (D) cases, and of CD44 in TB (E) and BCC (F) cases, respectively.
Fig 4: Survival time of patients with gastric cancer. DFS and OS curves. (A) DFS and (B) OS curves for ALDH1A1 expression. (C) DFS and (D) OS curves for CD133 expression. (E) DFS and (F) OS curves for ALDH1A1 and CD133 co-expression. (G) DFS and (H) OS curves for the two- and three-drug groups. DFS, disease-free survival; OS, overall survival; ALDH1A1, aldehyde dehydrogenase-1A1; CD133, cluster of differentiation 133.
Fig 5: Knockdown of CD133 expression in drug-resistant cell lines inhibits cell invasion. (A) Wound healing assay detected the migration of drug-resistant cell lines. (B) Transwell assay detected the invasion of drug-resistant cell lines. Images and statistical chart of cell invasion rate. **P<0.01, ***P<0.001. CD, cluster of differentiation; 5-FU, 5-fluorouracil; PYM, pingyangmycin; siRNA, small interfering RNA; NC, negative control.
Supplier Page from Abcam for Anti-CD133 antibody [EPR16508]