Fig 2: Knockdown of CCNB1 inhibits the growth of Caski and Siha cells and cell cycle progression in vitro. Three siRNAs targeting CCNB1 were delivered into Caski and Siha cells, which have the relative high expression of CCNB1. (A) The mRNA expression of CCNB1 in cells measured by RT-qPCR; (B) the protein expression of CCNB1 in cells measured by Western blot; (C) cell growth activity in Caski and Siha cells evaluated by Cell Tilter Glow kit; (D) the proliferative activity of Caski and Siha cells measured by EdU staining; (E) the number of colonies formed evaluated by colony formation assay; (F) cell apoptosis in Caski and Siha cells by flow cytometry; (G) cell cycle distribution in Caski and Siha cells by flow cytometry; (H) the expression of PCNA, Bcl-2, Bax, PUMA and Cleaved Caspase-3 in Caski and Siha cells measured by Western blot. The experiments were performed in triplicate and expressed as mean ± SD. Two-way analysis of variance followed by Tukey’s test were applied for statistical analysis. **p < 0.01, ***p < 0.001 vs siScr.