Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control ab15895 from Abcam

Product Specs
ItemAnti-VDAC1 / Porin antibody - Mitochondrial Loading Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab15895
Quantity100 UG, 250 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot, Immunocytochemistry/ Immunofluorescence, Western blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Research AreaNeuroscience
Gene NameVDAC1
ReactivityChicken, Chinese hamster, Dog, Human, Mouse, Rat
TargetVDAC1 / Porin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet sizeLife Sci AllianceDecember 6, 2023Alexandra J Brownstein, Michaela Veliova, Rebeca Acin-Perez, Frankie Villalobos, Anton Petcherski, Alberto Tombolato, Marc Liesa, Orian S Shirihaifollowing antibodies: MTCO1 antibody (1D6E1A8) (ab14705), Total Rodent OXPHOS Cocktail (ab110413), VDAC (ab15895) all from Abcam. ATP5A1 Monoclonal Antibody (15H4C4) (43-9800; Thermo Fisher Scientific), NDUFFB8 monoclonal

(2) Prominent muscle involvement in a familial form of mitochondrial disease due to a COA8 variantFrontiers in GeneticsNovember 30, 2023Martina Rimoldi, Francesca Magri, Sara Antognozzi, Michela Ripolone, Sabrina Salani, Daniela Piga, Letizia Bertolasi, Simona Zanotti, Patrizia Ciscato, Francesco Fortunato, et al.A21347, 1:1000) subunits. The mitochondrial PORIN (VDAC) was assayed by using a specific antibody (Abcam ab15895, 1:1500). Protein signals were detected using fluorescent secondary antibodies (LI-COR IR-DYE 800–680

(3) Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex VivoInternational Journal of Molecular SciencesNovember 4, 2023Tara Samra, Tatiana Gomez-Gomez, Kinga Linowiecka, Aysun Akhundlu, Gabriella Lopez de Mendoza, Matthew Gompels, Wendy W. Lee, Jennifer Gherardini, Jérémy Chéret, Ralf Pausmonoclonal anti-TFAM [EPR12285](1:500; Abcam, #ab176558), rabbit monoclonal anti-VDAC1/Porin (1:100; Abcam, #ab15895), rabbit monoclonal anti-MTCO1 [EPR19628] (1:50; Abcam, #ab203912), rabbit monoclonal anti-human Alexa

(4) Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatmentEuropean Journal of HistochemistryOctober 2, 2023Zhao Zhang, Hongming Fan, William Richardson, Bruce Z. Gao, Tong YeAntigen (origin),Dilution,Product no.,Vendor VDAC1 (rabbit),1:200,ab15895,Abcam CD31 (rat),1:100,DIA-310,Dianova Collagen type I (rabbit),1:200,600-401-103,Rockland Collagen

(5) MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosisNature communicationsAugust 10, 2023Ding Y, Gui X, Chu X, Sun Y, Zhang S, Tong H, Ju W, Li Y, Sun Z, Xu M, et al.paraformaldehyde and treated with 0.2% Triton followed by incubation with antibodies against VDAC (Abcam, ab15895, 1:200), 8-hydroxy-2′-deoxyguanosine (8-oxo-dG) (JaICA, N45.1, 1:50) or isotype control antibody IgG

(6) Triple Therapy with Metformin, Ketogenic Diet, and Metronomic Cyclophosphamide Reduced Tumor Growth in MYCN-Amplified Neuroblastoma XenograftsMetabolitesAugust 3, 2023Catalano L, Aminzadeh-Gohari S, Weber DD, Poupardin R, Stefan VE, Smiles WJ, Tevini J, Feichtinger RG, Derdak S, Bilban M, et al.(1:1000, 12252, Cell Signaling, Danvers, MA, USA), voltage-dependent anion channel (VDAC) (1:1000, ab15895, Abcam, Cambridge, UK), and β-actin (1:2000, ab8227, Abcam, Cambridge, UK). Horseradish peroxidase-labelled

(7) Pharmacological and Genetic Suppression of VDAC1 Alleviates the Development of Mitochondrial Dysfunction in Endothelial and Fibroblast Cell Cultures upon Hyperglycemic ConditionsAntioxidants (Basel)July 20, 2023Konstantin N. Belosludtsev, Dmitriy A. Serov, Anna I. Ilzorkina, Vlada S. Starinets, Mikhail V. Dubinin, Eugeny Yu. Talanov, Maxim N. Karagyaur, Alexandra L. Primak, Natalia V. Belosludtsevanonfat dry milk overnight at 4 °C and incubated with the appropriate primary antibody. The anti-VDAC1 (ab15895), anti-GAPDH (ab181602) were from Abcam. Enhanced chemiluminescence detection reagents (Pierce, Rockford,

(8) RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegiaThe Journal of clinical investigationJuly 17, 2023Launay N, Ruiz M, Planas-Serra L, Verdura E, Rodríguez-Palmero A, Schlüter A, Goicoechea L, Guilera C, Casas J, Campelo F, et al.1:1,000), anti-LC3B (Cell Signaling Technology, catalog 2775, 1:1,000), anti-VDAC1 (Abcam, catalog ab15895, 1:1,000), anti-Complex III (Molecular Probes, catalog A21362, 1:1,000), anti-Complex IV (Invitrogen,

(9) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJune 1, 2023Silver B, Gerrish K, Tokar E.anti-vinculin (Novus, NBP2-20859), rabbit anti-Nkx2.5 (Cell Signaling, 8792T), rabbit anti-VDAC (Abcam, ab15895), mouse anti-p53 (1:500; Novus, NBP2-29453), rabbit anti-MEF2C (Cell Signaling, 5030), rabbit anti-GATA-6

(10) Kidney-Related Function of Mitochondrial Protein MitoregulinInternational Journal of Molecular SciencesMay 22, 2023Olga A. Averina, Oleg A. Permyakov, Mariia A. Emelianova, Ekaterina A. Guseva, Olga O. Grigoryeva, Maxim L. Lovat, Anna E. Egorova, Andrei V. Grinchenko, Vadim V. Kumeiko, Maria V. Marey, et al.Waltham, MA, USA), anti-MFN2 (ab56889, Abcam, Waltham, MA, USA), anti-CYB5R3 (sc-398043), anti-VDAC1 (ab15895, Abcam, Waltham, MA, USA). The secondary HRP-conjugated anti-mouse (1721011, Biorad, Hercules, CA, USA)

(11) Proteostatic modulation in brain aging without associated Alzheimer’s disease-and age-related neuropathological changesAging (Albany NY)May 15, 2023Pol Andrés-Benito, Ignacio Íñigo-Marco, Marta Brullas, Margarita Carmona, José Antonio del del Rio, Joaquín Fernández-Irigoyen, Enrique Santamaría, Mónica Povedano, Isidro FerrerLMP7,Abcam,ab3329,Rb,1/500 ATG5,Cell Signaling,#12994,Rb,"1/1,000" GSK3α/β,Santa Cruz Biotech.,SC7291,Ms,"1/1,000" VDAC,Abcam,ab15895,Rb,1/500 ATP synthase,Biosciences,612516,Ms,"1/10,000" List of antibodies used for Western blotting

(12) Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cellsCell death & diseaseMay 13, 2023Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G, et al.,Anti-PGC1 alpha,Abcam,ab54481 4,Anti-MIRO1,Abcam,ab83779 5,Anti-LC3B,Abcam,ab48394 6,Anti-TOMM20,Abcam,ab56783 7,Anti-LAMP1,Abcam,ab25245 8,Recombinant Anti-Beclin 1,Abcam,ab207612 9,Anti-VDAC1/Porin,Abcam,ab15895 10,Anti-PGC1 alpha,Abcam,ab106814 11,Anti-PINK1,Abcam,ab75487 12,Anti-COX IV,Abcam,ab14744 13,Anti-FUNDC1,Abcam,ab74834 14,Anti-DRP1,Abcam,ab140494 15,Anti-SQSTM1/p62,Abcam,ab56416 16,Anti-Uteroglobin

(13) Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancerScience advancesApril 21, 2023Mukhopadhyay S, Encarnación-Rosado J, Lin EY, Sohn ASW, Zhang H, Mancias JD, Kimmelman AC.transferrin receptor (Abcam, ab84036), FPN/SLC40A1 (Novus Biologicals, NBP1-21502), VDAC1/porin (Abcam, ab15895), CIAPIN1 (Proteintech, 12638-1-AP), NUBP1 (Proteintech, 18011-1-AP), PTGS2 (Proteintech, 12375-1-AP),

(14) USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human NeuronsCellsApril 13, 2023Sofia Mauri, Greta Bernardo, Aitor Martinez, Mariavittoria Favaro, Marta Trevisan, Gael Cobraiville, Marianne Fillet, Federico Caicci, Alexander J. Whitworth, Elena Ziviani(1:500, Abcam ab110324), α-TOM20 (1:1000, Santa Cruz sc-11415, Dallas, TX, USA) and α-VDAC (1:1000, Abcam ab15895). Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare,

(15) Enhanced Ca(2+)-channeling complex formation at the ER-mitochondria interface underlies the pathogenesis of alcohol-associated liver diseaseNature communicationsMarch 27, 2023Thoudam T, Chanda D, Lee JY, Jung MK, Sinam IS, Kim BG, Park BY, Kwon WH, Kim HJ, Kim M, et al.(Molecular devices). Primary antibodies used in in situ PLA experiments were anti-rabbit VDAC1 (Abcam, ab15895; 1:100 dilution), anti-mouse monoclonal IP3R1 (Santa Cruz; sc-271197; Clone E8; 1:50 dilution), anti-mouse

(16) Neuronal loss of NCLX-dependent mitochondrial calcium efflux mediates age-associated cognitive declineiScienceMarch 17, 2023Jadiya P, Cohen HM, Kolmetzky DW, Kadam AA, Tomar D, Elrod JW.,Santa Cruz Biotechnology Inc.,Cat # sc-86337 NCLX (1:500),Santa Cruz Biotechnology Inc.,Cat # sc-161921 VDAC (1:2500),Abcam,Cat # ab15895 OxPhos Cocktail,Abcam,Cat # MS604 "Total APP (22C11, 1:1500)",Chemicon International,Cat # MAB348 BACE1

(17) Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse modelsMolecular therapy. Nucleic acidsMarch 14, 2023Guo CJ, Cao XL, Zhang YF, Yue KY, Han J, Yan H, Han H, Zheng MH.(Abcam, ab92729, rabbit), CD63 (Abcam, ab217345, rabbit), Alix (Abcam, ab186429, rabbit), Vdac1 (Abcam, ab15895, rabbit), and β-actin (GeneTex, GTX11003, mouse) were used. TUNEL assay kit was purchased from Roche.

(18) Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activationMolecular neurodegenerationMarch 7, 2023Todorova V, Stauffacher MF, Ravotto L, Nötzli S, Karademir D, Ebner LJA, Imsand C, Merolla L, Hauck SM, Samardzija M, et al.Abcam, ab14744), rabbit anti-CRE (1:300; Merck Millipore, 69050-3), rabbit anti-VDAC1 (1:250; Abcam, ab15895), rabbit anti-TFAM (1:250; Abcam, ab131607), rabbit anti-CS (1:250; GeneTex, GTX110624), rabbit anti-ACO2

(19) PERK recruits E-Syt1 at ER-mitochondria contacts for mitochondrial lipid transport and respirationThe Journal of cell biologyMarch 6, 2023Sassano ML, van Vliet AR, Vervoort E, Van Eygen S, Van den Haute C, Pavie B, Roels J, Swinnen JV, Spinazzi M, Moens L, et al.Sigma-Aldrich, SAB1303408; Rabbit polyclonal VDAC1, Cell Signaling, 4866S; Rabbit polyclonal VDAC1, Abcam, ab15895; Veriblot antibody Abcam, ab131366. The reagents used were: Antimycin A, Sigma-Aldrich, A8674; Calcium Chloride dihydrate

(20) TEFM variants impair mitochondrial transcription causing childhood-onset neurological diseaseNature CommunicationsFebruary 23, 2023Lindsey Van Haute, Emily O’Connor, Héctor Díaz-Maldonado, Benjamin Munro, Kiran Polavarapu, Daniella H. Hock, Gautham Arunachal, Alkyoni Athanasiou-Fragkouli, Mainak Bardhan, Magalie Barth, et al.OXPHOS system: NDUFA9 (1:500, ab14713, Abcam), MTCO1 (1:500, ab14705, Abcam), porin/VDAC1 (1:1000, ab15895, Abcam), ATP5A (1:1000, ab14748, Abcam), and β-actin (1:2000, A1978, Sigma-Aldrich). Membranes were

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9/30/2019Immunoblotting of Ubiquitylated Form and Unmodified Forms of VDAC1 Alban O.This antibody works great and has been very useful for my research. I'd recommend it, to immuno detect the endogenous unmodifed and Ub-modified form of VDAC.

Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control from Abcam

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Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control from Abcam

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