Anti-Chromogranin A antibody ab15160 from Abcam

Product Specs
ItemAnti-Chromogranin A antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab15160
Quantity1 ML, 100 UL, 500 UL
ApplicationsWestern blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Gene NameCHGA
ReactivityHuman
TargetChromogranin A

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) The Identification of CELSR3 and Other Potential Cell Surface Targets in Neuroendocrine Prostate CancerCancer Res CommunAugust 1, 2023Van Emmenis L, Ku SY, Gayvert K, Branch JR, Brady NJ, Basu S, Russell M, Cyrta J, Vosoughi A, Sailer V, et al.Santa Cruz Biotechnology #SC-374104), anti-SYP(1:500, Sigma-Aldrich #336R-94), anti-CHGA(1:2500, Abcam #ab15160). Multiplex CRISPR Knockout of CELSR3 in NEPC Organoids The sequence of single-guide RNA (sgRNA) targeting

(2) An enteroendocrine-microbial axis in the large intestine controls host metabolismResearch SquareJuly 7, 2023Shuai Burstein, Jacobo Santolaya, Tiffany Freeney Santolaya, Tiffany Wright, Teppei Fujikawa, Teppei Fujikawa, Colin P. Bergstrom, Colin Bergstrom, Adam Lopez, Goncalo Dias do Vale, et al.REAGENT OR RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Anti CHGA,Abcam,Cat# ab15160; RRID:AB_301704 Alexa555,Invitrogen,Cat# A-21428; RRID:AB_2535849 Chemicals,Chemicals,Chemicals Vancomycin,Sigma

(3) Single-cell chromatin accessibility of developing murine pancreas identifies cell state-specific gene regulatory programsMolecular MetabolismJuly 1, 2023Sean de la O, Xinkai Yao, Sean Chang, Zhe Liu, Julie B. Sneddonovernight at 4 C using primary antibodies against GFP (1:500, Abcam Cat. ab13970), Chga (1:250, Abcam Cat. ab15160), or E-cadherin (1:100, BD Transduction Laboratories Cat. 610182). The next day, sections were washed

(4) Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissuesDevelopment (Cambridge, England)July 1, 2023Singh P, Lanman NA, Kendall HLR, Wilson L, Long R, Franco OE, Buskin A, Miles CG, Hayward SW, Heer R, et al.sc-6260, 1:200), anti-human mitochondrial marker (Abcam, ab92824, 1:200), anti-chromogranin A (Abcam, Ab15160, 1:200), anti-neuregulin-2 (Santa Cruz Biotechnology, sc-398594, 1:100), phospho-HER2/ErbB2 (Cell Signaling Technology

(5) Faulty Metabolism: A Potential Instigator of an Aggressive Phenotype in Cdk5-dependent Medullary Thyroid CarcinomabioRxivJune 20, 2023Priyanka Gupta, Brendon Herring, Nilesh Kumar, Rahul Telange, Sandra S. Garcia-Buntley, Tessa W. Caceres, Simona Colantonio, Ford Williams, Pradeep Kurup, Angela M. Carter, et al.then incubated overnight at 4°C in primary antibodies to GFP (Cell Signaling 2956; 1:200), ChrA (Abcam ab15160; 1:1000), P-T202 LARP6 (Bibb Lab; 1:200), P-S17 H1.5 (Bibb Lab; 1:50), and P-S988 RBL1 (Bibb Lab; 1:100)

(6) PRMT5 promotes chemotherapy‐induced neuroendocrine differentiation in NSCLCThoracic CancerJune 1, 2023Qi Shen, Yi Liu, Xuehong Deng, Chang‐Deng Hu1:1000 dilution), anti‐γH2AX‐rabbit (Cell signaling, 9718S, 1:200 dilution), anti‐CgA‐rabbit (Abcam, ab15160, 1:500 dilution). Secondary antibodies used were FITC conjugated anti‐mouse IgG (Jackson ImmunoResearch,

(7) Using 3D-bioprinted models to study pediatric neural crest-derived tumorsInternational Journal of BioprintingMarch 29, 2023Colin H H. Quinn, Andee M M. Beierle, Janet R R. Julson, Michael E E. Erwin, Hasan Alrefai, Hooper R R. Markert, Jerry E E. Stewart, Sara Claire Claire Hutchins, Laura V V. Bownes, Jamie M M. Aye, et al.were purchased from Thermo Fischer Scientific. The antibodies used included rabbit antichromogranin A (ab15160) from Abcam (Cambridge, MA, USA) and rabbit antineuron-specific enolase (NSE) (AB951-I).Anti-rabbit

(8) The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryoPLOS GeneticsMarch 17, 2023Reyhaneh Tirgar, Jonathan P. Davies, Lars Plate, Jared T. NordmanGoat anti-Rabbit (Life technologies, A11034) was used at 1:500. For CldU, Goat anti-Rat 594 (Abcam, ab15160) was used at 1:350. Cells were rinsed three times with PBX then washed four times five minutes each.

(9) Intelectin-1 binds and alters the localization of the mucus barrier-modifying bacterium Akkermansia muciniphilaJ Exp MedJanuary 2, 2023Juan D. Matute, Jinzhi Duan, Magdalena B. Flak, Paul Griebel, Jose A. Tascon-Arcila, Shauni Doms, Thomas Hanley, Agne Antanaviciute, Jennifer Gundrum, Jessica L. Mark Welch, et al.at 4°C with rabbit anti-mouse ITLN1 (PAA933Mu01; Cloud Clone) at 1:400, rabbit anti-Chromogranin A (ab15160; Abcam) at 1:400, rabbit anti-DCLK1 (ab 37994; Abcam) 1:50, or sheep anti-human ITLN1 (AF4254; R&D Systems)

(10) Defining the early stages of intestinal colonisation by whipwormsNature CommunicationsDecember 1, 2022María A. Duque-Correa et al.α-villin (1:100, Abcam, ab130751), α-Ki-67 (1:250, Abcam, ab16667), α-chromogranin A (1:50, Abcam, ab15160), α-Dcamlk-1 (1:200, Abcam, ab31704), α-zona occludens-1 (ZO-1) protein (1:200, Invitrogen, 61-7300)

(11) Culture media composition influences patient-derived organoid ability to predict therapeutic responses in gastrointestinal cancersJCI InsightNovember 22, 2022Tara L. Hogenson, Hao Xie, William J. Phillips, Merih D. Toruner, Jenny J. Li, Isaac P. Horn, Devin J. Kennedy, Luciana L. Almada, David L. Marks, Ryan M. Carr, et al.anti-CDH1 (BD, 610181; 1:100), anti-synaptophysin (Abcam, ab32127; 1:250), anti-chromogranin (Abcam, ab15160; 1:100), and anti-SOX9 (EMD, AB5535; 1:500). Secondary antibodies were goat anti–rabbit IgG (H+L), Alexa

(12) Defining the impact of platelet-to-lymphocyte ratio on patient survival with gastric neuroendocrine neoplasm: a retrospective cohort analysisWorld J Surg OncolNovember 9, 2022Wenquan Liang, Xinxin Xu, Yuhua Liu, Jianxin Cui, Yunhe Gao, Chuang Wang, Ziwei Zhuang, Kecheng Zhang, Hongqing Xi, Aizhen Cai, et al.or cumulative smoking for 6 months or more in a lifetime were defined as smokers. Anti-CgA antibody (ab15160) and anti-Syn antibody (ab32127) were obtained from Abcam Trading Company, and two pathologists independently

(13) Transit-amplifying cells control R-spondins in the mouse crypt to modulate intestinal stem cell proliferationThe Journal of experimental medicineNovember 7, 2022Chaves-Pérez A, Santos-de-Frutos K, de la Rosa S, Herranz-Montoya I, Perna C, Djouder N.mouse), rabbit polyclonal anti-GPCR GPR49 (ab75732, 1:200), rabbit polyclonal anti-Chromogranin A (ab15160, 1:100 [IHC]) were purchased from Abcam. Rabbit polyclonal anti-Mex3a (ab79046, 1:50 [IF]) was provided

(14) Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mouse and Syrian golden hamster modelsDisease models & mechanismsNovember 1, 2022Jeong H, Woo Lee Y, Park IH, Noh H, Kim SH, Kim J, Jeon D, Jang HJ, Oh J, On D, et al.anti-Ly-6G/Ly-6C (ab2557, Abcam, 1:250), anti-p-AKT (9271S, Cell Signaling Technology, 1:500), anti-CHGA (ab15160, Abcam, 1:1000), anti-CD4 (25229S, Cell Signaling Technology, 1:200) and anti-CD8 (14-0808-82, Thermo

(15) Application of immune enhanced organoids in modeling personalized Merkel cell carcinoma researchScientific reportsAugust 16, 2022Forsythe SD, Erali RA, Laney P, Sivakumar H, Li W, Skardal A, Soker S, Votanopoulos KI.13-1300, Thermofisher, Waltham, MA), Synaptophysin (SYP, MA5-14532, Thermofisher), Chromogranin A (ChgA, ab15160, Abcam), and Merkel Cell Polyomavirus T-Antigen (MCPyV, MABF2316, Millipore Sigma) primary antibodies

(16) Development of a Primary Human Intestinal Epithelium Enriched in L-Cells for Assay of GLP-1 SecretionAnalytical chemistryJuly 12, 2022Villegas-Novoa C, Wang Y, Sims CE, Allbritton NL.at 25 °C for 1 h. The antibodies used for immunodetection were against chromogranin A (ChgA) (Abcam, ab15160, rabbit, 1: 1000), peptide YY (PYY) (Proteintech, 24294-1AP, rabbit 1:200), GLP-1 (Santa Cruz, sc-514592,

(17) Human 2D Crypt Model for Assaying Intestinal Stem Cell Proliferation and DifferentiationAnalytical chemistryJuly 5, 2022Wang Y, Sims CE, Allbritton NL.subcellular structures, the cells were stained with the enteroendocrine cell marker chromogranin A (Abcam, #ab15160, 1:1000), the proliferative cell marker Ki67 (Fisher, #PA1-21520, 1:200), a pan-differentiation marker

(18) Cell and chromatin transitions in intestinal stem cell regenerationGenes & DevelopmentJune 1, 2022Pratik N.P. Singh, Shariq Madha, Andrew B. Leiter, Ramesh A. Shivdasani4583), and 10-µm sections were prepared using a Leica cryostat. Incubation with CHGA (1:100; Abcam ab15160), LYZ1 (1:1000; Dako A0099), 5-hydroxy tryptamine (1:500; Immunostar 20080), MUC2 (1:1000; Santa Cruz Biotechnology

(19) Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroidsPLoS pathogensJune 1, 2022Yang QY, Yang YL, Tang YX, Qin P, Wang G, Xie JY, Chen SX, Ding C, Huang YW, Zhu SJ.anti-Ki-67 (Affinity, AF0198), anti-villin (Proteintech, 16488-1-AP) or anti-chromogranin A (Abcam, ab15160). For endosome and lysosome detection, anti-Rab5 (Proteintech, 11947-1-AP), anti-Rab7 (Proteintech,

(20) Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epitheliumCell regeneration (London, England)May 5, 2022Li H, Wang X, Wang Y, Zhang M, Hong F, Wang H, Cui A, Zhao J, Ji W, Chen YG.Microscope. Antibodies Rabbit anti-Muc2 (1:200, sc-15,334; Santa Cruz), rabbit anti-ChgA (1:200, ab15160; Abcam), rabbit anti-Ki67 (1:200, ab15580; Abcam), rabbit anti-Lyz (1:200, ab108508; Abcam), mouse anti-E

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Anti-Chromogranin A antibody from Abcam

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Anti-Chromogranin A antibody from Abcam

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