Fig 1: The structure of Campaniform receptor in haltere and the subcellular localization of NOMPC in campaniform receptor in the haltereA: SEM image of a haltere and a receptor field (green in inset) in the pedicel of the haltere (pink in inset). Scale bar: 100 µm. B: High magnification SEM image of a campaniform receptor field in the haltere pedicel. C: Transmission electron microscopy image of a longitude section of a campaniform receptor in the haltere from a nompC3 fly. The whole cell region and nucleus region are highlighted in light green and light blue, respectively. It shows that campaniform receptor in nompC3 fly has a similar overall shape to that of wild type neurons [Keil,1997]. The inset shows an enlargement of the tubular body in this campaniform receptor. D: Schematic diagram of a campaniform receptor and the tubular body. Inset: the distal tip region of the campaniform receptor is clearly visualized with a mouse anti-acetylated tubulin antibody (Sigma, T6793). E: Anti-NOMPC antibody (red) exclusively stains the distal tips of tubular body, here visualized with an anti-a-tubulin antibody (green) (Abcam, Catalog No. ab15246). The inset shows a top-view of anti-NOMPC staining (red) in the distal tip of the tubular body. F: The same region of the tubular body (anti-a-tubulin, green) in the nompC3 fly did not stain with anti-NOMPC antibody. The distal tip region is not as strongly stained by the anti-a-tubulin as it is by the anti-acetylated tubulin antibody (D, inset) (Supporting Information Fig. S1).
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