Anti-CD31 antibody ab28364 from Abcam

Product Specs
ItemAnti-CD31 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab28364
Quantity1 ML, 100 UL, 500 UL
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Research AreaImmuno-oncology
Gene NamePecam1
ReactivityHuman
TargetCD31

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) miRNA-ome plasma analysis unveils changes in blood-brain barrier integrity associated with acute liver failure in ratsFluids Barriers CNSDecember 8, 2023Karolina Orzeł-Gajowik, Krzysztof Milewski, Magdalena Zielińskapermeabilized with 0.25% Triton X-100/PBS. Secondly, were incubated with antibodies against CD-31 (1:400, ab28364 Abcam, Cambridge, UK), vWF (1:400, ab6994, Abcam, Cambridge, UK) overnight at 4 °C and then exposed

(2) PCSK9 activation promotes early atherosclerosis in a vascular microphysiological systemAPL BioengineeringDecember 1, 2023Jounghyun H. Lee, Kevin L. Shores, Jason J. Breithaupt, Caleb S. Lee, Daniella M. Fodera, Jennifer B. Kwon, Adarsh R. Ettyreddy, Kristin M. Myers, Benny J. Evison, Alexandra K. Suchowerska, et al.1:100), calponin (Abcam—ab46794, 1:100), MYH11 (Santa Cruz Biotechnology—sc-6956, 1:100), CD31 (Abcam—ab28364, 1:100), VWF (Abcam—ab778, 1:100), and ICAM-1 (Santa Cruz Biotechnology—sc-8439, 1:100). After overnight

(3) Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesisBioactive MaterialsDecember 1, 2023Wenqian Zhang, Kangkang Zha, Yuan Xiong, Weixian Hu, Lang Chen, Ze Lin, Chenyan Yu, Wu Zhou, Faqi Cao, Hankun Hu, et al.followed by blocking for 30 min in goat serum. The samples were stained with anti-CD31 (1:100; Abcam, ab28364) antibodies overnight at 4 °C, washed in PBS and stained and counter-stained with DAB and hematoxylin,

(4) Injectable Microparticle-containing hydrogel with controlled release of bioactive molecules for facial rejuvenationMater Today BioDecember 1, 2023Semi Lee, Seung-Woon Baek, Da-Seul Kim, So-Yeon Park, Jun Hyuk Kim, Ji-Won Jung, Jun-Kyu Lee, Gi-Min Park, Chun Gwon Park, Dong Keun Han5′-caggctgctgtaacgatgaa-3′; 5′-gctttggtagggtttgatcc-3′). Immunofluorescence staining was performed with anti-CD31 (ab28364, Abcam 1:500) and VCAM-1 (sc-13160, Santa Cruz Biotechnology, USA, 1:100) as primary antibodies (4 °C

(5) Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex®Pediatric Surgery InternationalNovember 24, 2023Marianna Scuglia, Laura P. Frazão, Alice Miranda, Albino Martins, Joana Barbosa-Sequeira, Diana Coimbra, Adhemar Longatto-Filho, Rui L. Reis, Cristina Nogueira-Silva, Nuno M. Neves, et al.anti-alpha smooth muscle actin (anti α-SMA; 1:50, #ab5695; Abcam, Cambridge, UK), rabbit anti-CD31 (1:50, #ab28364; Abcam) and mouse anti-CD105 (1:100, #MCA1557F; BioRad, California, USA) for 2 h at room temperature.

(6) Exosomal miR-146a-5p derived from human umbilical cord mesenchymal stem cells can alleviate antiphospholipid antibody-induced trophoblast injury and placental dysfunction by regulating the TRAF6/NF-κB axisJournal of NanobiotechnologyNovember 13, 2023Qingfeng Lv, Yuan Wang, Wei Tian, Yuqiu Liu, Mengqi Gu, Xiaotong Jiang, Yanjun Cai, Ruiheng Huo, Yuchen Li, Lei Li, et al.specimen. Relative average density was quantified using ImageJ software. The antibodies informations: CD31 (ab28364, 1:50, Abcam), TRAF6 (ab137452, 1:100, Abcam), and NF-κB p65 (ab32536, 1:1000, Abcam). Immunofluorescence

(7) MiR-196b-5p activates NF-κB signaling in non-small cell lung cancer by directly targeting NFKBIATranslational OncologyNovember 1, 2023Wangyu Zhu, Yun Yu, Yuxin Ye, Xinyue Tu, Yan Zhang, Tao Wu, Lianli Ni, Xiangjie Huang, Yumin Wang, Ri Cuipurchased from Santa Cruz Biotechnology (Santa Cruz, USA). Antibodies against Ki67 (ab16667), CD31 (ab28364), and QKI-5 (ab232502) were purchased from Abcam (Cambridge, USA). Rabbit secondary antibody conjugated

(8) Glycerol-weighted chemical exchange saturation transfer nanoprobes allow 19F/1H dual-modality magnetic resonance imaging-guided cancer radiotherapyNature CommunicationsOctober 20, 2023Rong A, Haoyu Wang, Chaoqun Nie, Zhaoguo Han, Meifang Zhou, Olagbaju Oluwatosin Atinuke, Kaiqi Wang, Xiance Wang, Shuang Liu, Jingshi Zhao, et al.radiotherapy system (VARIAN CLINAC 21EX, US). Ki67(Abcam, ab15580, 0.5 μg/mL) and CD31 antibodies (Abcam, ab28364, 1:50 dilution) and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay (TUNEL, Beyotime

(9) Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted heartsBioactive MaterialsOctober 1, 2023Yun Jiang, Ling-Ling Zhang, Fan Zhang, Wei Bi, Peng Zhang, Xiu-Jian Yu, Sen-Le Rao, Shi-Hui Wang, Qiang Li, Chen Ding, et al.positive (α-SMA, Abcam, ab5694, 1:400), platelet endothelial cell adhesion molecule-1(CD31, Abcam, ab28364, 1:200), ki67 (Abcam, ab16667, 1:200), phosphorylated histone 3 (PH3, CST, #9701, 1:100), Aurora B kinase

(10) Sensing Cytosolic DNA Lowers Blood Pressure by Direct cGAMP-Dependent PKGI ActivationCirculationSeptember 26, 2023Jie Su, Pierre Coleman, Angeliki Ntorla, Rhys Anderson, Michael J. Shattock, Joseph R. BurgoyneTBK1 (Cell Signaling, no. 3013), Phospho-STING (Ser365; Cell Signaling, no. 72971), CD31 (Abcam, no. ab28364), MRP1 (Santa Cruz, no. sc-18835), LRRC8A (Santa Cruz, no. sc-517113), GAPDH (Cell Signaling, no. 2118),

(11) LFA-1 knockout inhibited the tumor growth and is correlated with treg cellsCell Communication and SignalingSeptember 18, 2023Ting Niu, Zhengyang Li, Yiting Huang, Yuxiang Ye, Yilong Liu, Zhijin Ye, Lingbi Jiang, Xiaodong He, Lijing Wang, Jiangchao Lisolution. The primary antibodies included anti-Ki67 (1:100, ab16667, Abcam, USA), anti-CD31 (1:100, ab28364, Abcam, USA), and anti-Foxp3 (1:100, BA2032-1, Boster, China) antibodies. The HRP-conjugated anti-rabbit/mouse

(12) Differential systemic decellularization in vivo to study molecular changes in each vasculature layer in murine models of diseaseSTAR ProtocolsSeptember 1, 2023Xavier Gallart-Palau, Cristina Lorca, María Mulet, José Antonio Sánchez Milán, Julia Lisa, SoFong Cam Ngan, Ranjith Iyappan, Maryam Katoueezadeh, Aida Serra, Siu Kwan SzeKetamine hydrochloride/xylazine hydrochloride solution,Merck,K113 ,, Antibodies,Antibodies,Antibodies ,, Rabbit polyclonal anti-PECAM1 (10 μg/mL),Abcam,ab28364 Alexa Fluor anti-rabbit 546-conjugated secondary antibody (2 μg/mL),Jackson ImmunoResearch,A-11035 Alexa

(13) hESC-Derived Epicardial Cells Promote Repair of Infarcted Hearts in Mouse and SwineAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Luo XL, Jiang Y, Li Q, Yu XJ, Ma T, Cao H, Ke MX, Zhang P, Tan JL, Gong YS, et al.sections were fixed with 4% PFA, permeabilized with 0.4% Triton X‐100, and stained with anti‐CD31 (Abcam, ab28364), vWF (Dako, A0082), LYVE1 (R&D systems, AF2125), CD11b (Abcam, ab8878), CD68 (Abcam, ab53444; Immunoway,

(14) Delivery of multipotent adult progenitor cells via a functionalized plasma polymerized surface accelerates healing of murine diabetic woundsFrontiers in Bioengineering and BiotechnologyAugust 22, 2023S. J. Mills, G. T. Kirby, B. R. Hofma, L. E. Smith, P. Statham, B. Vaes, A. E. Ting, R. Short, A. J. CowinInc. (United States), VEGF (MA1-16626) was from Thermo Fisher Scientific (MA, United States) and CD31 (ab28364) and HNA antibody (ab215396) was from Abcam (Cambridge, UK). All secondary antibodies were Alexa Fluors,

(15) Gene Immunotherapy of Colon Carcinoma with IL-2 and IL-12 Using Gene ElectrotransferInt J Mol SciAugust 17, 2023Tilen Komel, Masa Omerzel, Urska Kamensek, Katarina Znidar, Ursa Lampreht Tratar, Simona Kranjc Brezar, Klemen Dolinar, Sergej Pirkmajer, Gregor Sersa, Maja Cemazaranti-mouse CD4 (ab183685), 1/1000 for rabbit anti-mouse CD8 (ab209775), 1/200 for rabbit anti-mouse CD31 (ab28364) (Abcam, Cambridge, UK) and 1/200 for rabbit anti-mouse CD11c (D1V9Y) (Cell Signalling Technology, Danvers,

(16) Highly reproducible rat arterial injury model of neointimal hyperplasiaPLoS OneAugust 17, 2023Richard P. Tan, Jui Chien Hung, Alex H. P. Chan, Angus J. Grant, Matthew J. Moore, Yuen Ting Lam, Praveesuda Michael, Steven G. Wiseprotocols. For immunohistochemistry staining, sections were stained with antibodies against CD31 (Abcam, ab28364, 1:200) for endothelialisation, SMCα (Abcam, ab5694, 1:200) and PCNA for smooth muscle cell contractile

(17) Camrelizumab combined with apatinib in patients with first-line platinum-resistant or PD-1 inhibitor resistant recurrent/metastatic nasopharyngeal carcinoma: a single-arm, phase 2 trialNat CommunAugust 14, 2023Li Yuan, Guo-Dong Jia, Xiao-Fei Lv, Si-Yi Xie, Shan-Shan Guo, Da-Feng Lin, Li-Ting Liu, Dong-Hua Luo, Yi-Fu Li, Shen-Wen Deng, et al.1:200); α-SMA (ab7817, Abcam, 1μg/mL); KDR (ab2349, Abcam, 1:100); PD-L1 (ab205921, Abcam, 2μg/mL); CD31(ab28364, Abcam, 1:50); IL8 (94407 T, Cell Signaling Technology, 1:100); VEGFA (ab52917, Abacm, 1:100); c-KIT

(18) Synergistic efficacy of simultaneous anti-TGF-β/VEGF bispecific antibody and PD-1 blockade in cancer therapyJournal of hematology & oncologyAugust 12, 2023Niu M, Yi M, Wu Y, Lyu L, He Q, Yang R, Zeng L, Shi J, Zhang J, Zhou P, et al.targeting E-cadherin (3195, CST), Vimentin (5741, CST), N-cadherin (13116, CST), α-SMA (19245, CST), CD31 (ab28364, Abcam), CD8 (98941, CST) were used in the assay. Images of IF were captured by fluorescence microscopy,

(19) Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healingNat CommunAugust 7, 2023Dominic Henn, Dehua Zhao, Dharshan Sivaraj, Artem Trotsyuk, Clark Andrew Bonham, Katharina S. Fischer, Tim Kehl, Tobias Fehlmann, Autumn H. Greco, Hudson C. Kussie, et al.5% goat serum (Invitrogen, Waltham, MA) in PBS. Sections were then incubated in anti-CD31 antibody (ab28364; Abcam, Waltham, MA) or anti-Ndrg2 antibody (ab174850; Abcam, Waltham, MA) at a 1:100 dilution overnight

(20) Immunomorphogenesis in Degenerative Disc Disease: The Role of Proinflammatory Cytokines and Angiogenesis FactorsBiomedicinesAugust 3, 2023Natalya G. Pravdyuk, Anna V. Novikova, Nadezhda A. Shostak, Anastasiia A. Buianova, Raisa T. Tairova, Olga I. Patsap, Aleksandr P. Raksha, Vitaliy T. Timofeyev, Victor M. Feniksov, Dmitriy A. Nikolayev, et al.anti-VEGF-A (1:500 dilution; Cloud-Clone Corp., Katy, TX, USA) and primary rabbit anti-CD31 (1:150 dilution; ab28364, Abcam, Cambridge, UK) were used. To identify processes of inflammation, we also applied primary mouse

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(1)

3/6/2023Time Course Effect of Chemoattractant on CD31 Expression Somasundaram RaghavanPECAM-1 otherwise called CD31is a protein that in humans is encoded by the PECAM1. It is one of the highly glycosylated protein with size of 130 kDa. PECAM-1 plays a key role in removing aged ... Read Review
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11/16/2022Excellent Antibody for CD31-Pecam-1 Immunostaining Filippo MassaCD31 is expressed in the surface of the endothelial cell forming the capillary system. This antibody gives a very sharp and string signal in immunoflourescent imaging of renal tissues vasculature.
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9/21/2022Anti-CD31 Antibody Works Well Liyong Z.The protein expression was examined using Western blotting and immunohistochemical (IHC) staining of angiogenesis marker CD31 in tumors obtained from in vivo tumorigenesis experiments. The results ... Read Review
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2/4/2022CD31 Antibody for IHC/IF Matthew RatsepThis is a good antibody for IHC/IF applications.
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8/9/2019Good CD31 for Immunofluorescence (Abcam)Dr. Efstathios StamatiadesGood rabbit polyclonal antibody for CD31.
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9/20/2018Anti-CD31 for Paraffin Embedded Samples Adam KramerThis antibody was used to monitor angiogenesis during wound healing in murine full excision skin wounds. Samples were taken at multiple time points and endothelial cells were stained using this CD31 ... Read Review

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Anti-CD31 antibody from Abcam

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Anti-CD31 antibody from Abcam

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