Fig 1: Organization of cells into ductal and stromal compartments in 3D organoidsA. Hematoxylin and Eosin tissue staining depicting formation of pancreatic ducts adjacent to one another (Supplementary Figure 2A, lower magnification, 200x). B. Hematoxylin and Eosin tissue staining demonstrating a well-formed pancreatic duct surrounded by pancreatic stellate cells (Supplementary Figure 2B, lower magnification, 200x). C. Immunohistochemistry demonstrating pancreatic ductal cells stained using anti-CK19 antibody (1:40, TROMA-III, University of Iowa, IA). D. Immunohistochemistry demonstrating activated pancreatic stellate cells stained with anti-alpha-SMA antibody (1:500, ab7817, Abcam, Cambridge, MA).
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