Anti-beta Catenin antibody [12F7] ab22656 from Abcam

Product Specs
ItemAnti-beta Catenin antibody [12F7]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab22656
Quantity10 UG, 100 UG
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
HostMouse, Nude mouse
Antibody TypePrimary
ClonalityMonoclonal
Clone12F7
Gene NameCTNNB1
ValidationKnockOut Validated
ReactivityHuman
Targetbeta Catenin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growthMolecular OncologyNovember 1, 2023Yagmur Azbazdar, Yeliz Demirci, Guillaume Heger, Dogac Ipekgil, Mustafa Karabicici, Gunes Ozhancatenin (Ser675, 1 : 100, D2F1, Cell Signaling Technology, MA, USA), mouse anti‐β‐catenin (1 : 100, 12F7, ab22656, Abcam, MA, USA), rabbit Biotin‐conjugated anti‐DAG (1 : 500, MBS2013372, MyBioSource Inc., CA, USA),

(2) DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathwayAmerican Journal of Physiology-Cell PhysiologyOctober 1, 2023Qingqing Wang, Fengxia Chen, Ningning Yang, Lu Xu, Xiaoyan Yu, Meng Wu, Yunfeng Zhouprimary antibodies against E-cadherin (1:100, no. Ab181; Abcam, United States) and β-catenin (1:100, no. Ab22656; Abcam) were incubated overnight at 4°C. The nuclei were stained with DAPI (no. H-1200; Vector, United

(3) The global Protein-RNA interaction map of ESRP1 defines a post-transcriptional program that is essential for epithelial cell functioniScienceOctober 21, 2022Peart NJ, Hwang JY, Quesnel-Vallières M, Sears MJ, Yang Y, Stoilov P, Barash Y, Park JW, Lynch KW, Carstens RP.,Thermo Fisher,"Cat# 36–4800, RRID: AB_2533262" Anti-Cdh1,Cell Signalling,Cat # 610182 Anti-Ctnnb1,Abcam,Cat # ab22656 Anti-Cttn,Abcam,Cat # ab81208 ECL Anti-mouse IgG,GE Healthcare,"Cat# NA931, RRID:AB_772210" ECL Anti-rabbit

(4) Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin SignalingBiomedicinesApril 18, 2022Ju-Hye Choi, Yu-Mi Kim, Hee-Jung Park, Myeong-Hyun Nam, Young-Kwon Seomonoclonal antibodies against ALP (diluted 1:50, ab75699, Abcam, Cambridge, MA, USA), β-catenin (1:200, ab22656, Abcam, Cambridge, MA, USA), and TUNEL (11684817910, Roche, Branford, CT, USA) were used in our analyses.

(5) Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal TransitionJournal of the American Heart AssociationApril 5, 2022Alvandi Z, Nagata Y, Passos LSA, Hashemi Gheinani A, Guerrero JL, Wylie-Sears J, Romero DC, Morris BA, Sullivan SM, Yaghoubian KM, et al.No. M0851), anti‐CD31 (1:50, Novus Biologicals; No. NB10001642), and anti‐β‐Catenin (1:40, Abcam; No. ab22656), overnight at 4 °C. Secondary conjugated antibodies were Alexa Fluor anti‐rabbit 647, Alexa Fluor anti‐rabbit

(6) Contractile and Structural Properties of Detrusor from Children with Neurogenic Lower Urinary Tract DysfunctionBiology (Basel)September 3, 2021Navroop Johal, Kevin X. Cao, Boyu Xie, Michael Millar, Reena Davda, Aamir Ahmed, Anthony J. Kanai, Dan N. Wood, Rita I. Jabr, Christopher H. FrypH-dependence and antigen retrieval. Those used, as per manufacturer’s instructions, were: β-catenin (ab22656, Abcam, Cambridge, UK), c-Myc (Novacastra/Leica Biosystems), cyclin-D1 (sc 718, Santa Cruz, Santa Cruz,

(7) Full-length isoform transcriptome of the developing human brain provides further insights into autismCell reportsAugust 31, 2021Chau KK, Zhang P, Urresti J, Amar M, Pramod AB, Chen J, Thomas A, Corominas R, Lin GN, Iakoucheva LM.AB_10795284 anti-V5 antibody,Thermo Fisher Scientific Cat# R960-25,RRID: AB_2556564 anti-β-catenin,Abcam Cat# ab22656,RRID: AB_447227 anti-p-βcatenin,Cell Signaling Technology Cat# 9561,RRID: AB_331729 anti-Cul1,Abcam

(8) CLCA2 suppresses the proliferation, migration and invasion of cervical cancerExperimental and therapeutic medicineJuly 1, 2021Zhang P, Lin Y, Liu Y.against CLCA2 (1:5,000, cat. no. HPA047192; Sigma‑Aldr ich; Merck KGa A), β ‑catenin (1:4,0 0 0; cat. no. ab22656; Abcam). The membranes were then incubated with HRP‑conjugated anti‑rabbit secondary antibody (1:4,000;

(9) SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial–mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancerCell Death & DiseaseMay 1, 2021Yin Zhu, Shimiao Huang, Shengyuan Chen, Jiaxuan Chen, Zhiqing Wang, Yadong Wang, Haoxuan Zheng(1:1000; Cell Signaling Technology, 23064S), ABCC2 (1:1000; Abcam, ab3373), β-catenin (1:1000; Abcam, ab22656), CD133 (1:1000; Abcam, ab19898), Snail (1:1000; Cell Signaling Technology, 3879S), Vimentin (1:1000;

(10) Graphitic Carbon Nitride Causes Widespread Global Molecular Changes in Epithelial and Fibroblast CellsACS OmegaApril 13, 2021Chatterjee Amit, Gajanan Sathe, Abinaya Shunmugam, Prasanna Kumar Athyala, Vivek Ghose, Srujana Chitipothu, Narayanan Janakiraman, Ramaprabhu Sundara, Sailaja V. ElchuriSDS PAGE and blotted onto the nitrocellulose membrane. After blocking primary antibodies β catenin (Abcam-ab22656), E cadherin (CST Cat No. 9961) were treated overnight followed by addition of a secondary antibody

(11) Contractile function of detrusor smooth muscle from children with posterior urethral valves – The role of fibrosisJournal of Pediatric UrologyFebruary 1, 2021Navroop Johal, Kevin Cao, Callum Arthurs, Michael Millar, Christopher Thrasivoulou, Aamir Ahmed, Rita I. Jabr, Dan Wood, Peter Cuckow, Christopher H. Frysectioned (4 μm). Antibodies for MMP-7 (ab4044, Abcam), cyclin-D1 (sc 718, Santa Cruz), β-catenin (ab22656, Abcam) and c-Myc (Novacastra/Leica Biosystems) were optimised for concentration, pH-dependence and

(12) lncRNA PART1, manipulated by transcriptional factor FOXP2, suppresses proliferation and invasion in ESCC by regulating the miR‑18a‑5p/SOX6 signaling axisOncology ReportsJanuary 11, 2021Yan Zhao, Qing Zhang, Hongtao Liu, Ning Wang, Xiaosan Zhang, Shujun Yangskimmed milk and incubated with primary antibodies against SOX6 (cat. no. ab84880), β-catenin (cat. no. ab22656), c-myc (cat. no. ab17355) and β-actin (cat. no. ab8226) (1:200 dilution, Abcam) overnight at room temperature.

(13) Oncogenic UBE3C promotes breast cancer progression by activating Wnt/β-catenin signalingCancer cell internationalJanuary 6, 2021Hang C, Zhao S, Wang T, Zhang Y.antibodies specific for UBE3C (1:1000 dilution, Cat. ab101512, Abcam), β-catenin (1:1000 dilution, Cat. ab22656, Abcam), Cyclin D1 (1:1000 dilution, Cat. ab40754, Abcam), and MMP9 (1:1000 dilution, Cat. ab76003,

(14) Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT AxisAnalytical cellular pathology (Amsterdam)January 1, 2021Tang Y, Weng X, Liu C, Li X, Chen C.albumin for 1 h, the membranes were incubated with the primary antibodies to β-catenin (1 : 1,000, ab22656, Abcam), p-AKT (1 : 500, 66444-1-Ig, Proteintech Group, Chicago, IL, USA), AKT (1 : 800, 60203-2-Ig,

(15) microRNA-214 Prevents Traits of Cutaneous Squamous Cell Carcinoma via VEGFA and Bcl-2Technology in Cancer Research & TreatmentDecember 7, 2020Xianpeng Ma, Di Wu, Xiaodong Zhang, Xiao Shao, Guangyao Huprimary antibodies to Cyclin D1 (sc-8396, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), β-catenin (ab22656, Abcam, Cambridge, UK) and β-actin (sc-81178, Santa Cruz Biotechnology) at 4°C. The secondary goat anti-mouse

(16) Deciphering the mechanoresponsive role of β-catenin in keratoconus epitheliumScientific ReportsDecember 1, 2020Chatterjee Amit, Prema Padmanabhan, Janakiraman NarayananNo-9961) β-Catenin for Co-IP (Cell Signaling Technology Cat No-9562S), β-Catenin for immunoblotting (Abcam-ab22656) ZO1, Wnt5ab, RhoA, Rac1/2/3, α-Actinin (Cell signaling and Technology Cat No-13663, 2520 T, 21,170,

(17) Agaricus blazei extract (FA-2-b-β) induces apoptosis in chronic myeloid leukemia cellsOncology lettersNovember 1, 2020Sun Y, Cheng M, Dong L, Yang K, Ma Z, Yu S, Yan P, Bai K, Zhu X, Zhang Q.GB‑12001; Beijing Biosynthesis Biotechnology Co., Ltd.), anti‑β‑catenin antibody (1:1,000; cat. no. ab22656; Abcam). The HRP‑linked goat anti‑rabbit IgG (1:4,000; cat. no. 7074) or goat anti‑mouse IgG (1:1,000;

(18) Vitamin D receptor knockdown attenuates the antiproliferative, pro‑apoptotic and anti‑invasive effect of vitamin D by activating the Wnt/β‑catenin signaling pathway in papillary thyroid cancerMolecular Medicine ReportsSeptember 17, 2020Rui Pang, Ye Xu, Xiaonan Hu, Bo Liu, Jiawei Yupolyclonal Antiâ€‘Wnt3; mouse polyclonal Antiâ€‘CTNNB1; mouse monoclonal Antiâ€‘GAPDH; mouse monoclonal ab89626 ab169175 ab22656 ab8245 1:1,000 1:1,000 1:1,500 1:5,000 ab205719 1:10,000 B, Secondary antibodya Goat antiâ€‘mouse IgGâ€‘HRP

(19) Melatonin suppresses chronic restraint stress-mediated metastasis of epithelial ovarian cancer via NE/AKT/β-catenin/SLUG axisCell death & diseaseAugust 18, 2020Bu S, Wang Q, Sun J, Li X, Gu T, Lai D.SNAIL (Rb, 1:100; Abcam, ab216347), β-catenin (Rb, 1:200; Abcam, ab32572), β-catenin (Ms, 1:200; Abcam, ab22656), and NE (Rb, 1:100; Abcam, ab8887). Next day, tissues were stained with fluorescent secondary antibodies

(20) Mediator Condensates Localize Signaling Factors to Key Cell Identity GenesMolecular CellDecember 1, 2019Alicia V. Zamudio et al.CCD camera. Primary antibodies used were anti-MED1 Abcam ab64965 1:500 dilution, anti-b catenin Abcam ab22656 1:500 dilution, anti-pSTAT3 Santa Cruz 1:20 dilution, anti-SMAD2/3 Santa Cruz 1:20 dilution). Secondary

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Anti-beta Catenin antibody [12F7] from Abcam

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Anti-beta Catenin antibody [12F7] from Abcam

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