Fig 1: JNK3 is upregulated and involved in RGC death after ONA in mice a Quantitative analysis of relative mRNA levels of Jnks in intact retinae or retinae 1 day (1 d), 1 week (1 w) or 2 weeks (2 w) after ONA (n = 3 for each time point). b Representative western blots from at least three biological repeats showing JNK expression after ONA. Intact retina or retinae 1 d, 1 w, 2 w after ONA were harvested, lysed and immunoblotted using antibodies against JNK1, JNK2, JNK3, pJNK or GAPDH. The location of molecular weight markers is shown in kDa on the right. c Representative fluorescence microscope images show immunostaining of mouse retina flat-mounts from wild-type or JNK1, JNK2, or JNK3-null mice (n = 6 for each genotype) 14 days after ONA using an anti-Tuj1 antibody. d The bar chart shows quantitative analysis of Tuj1+ RGC numbers 14 days after ONA. Scale bar, 50 µm. One-way ANOVA followed by post-hoc Bonferroni test: **p < 0.01. Error bars represent SEM