Fig 2: Localization of CLIC1, CLIC4 and CLIC5 to the endoplasmic reticulum. (I) Isolated p3 neonatal cardiomyocytes were loaded with ER tracker (A, E, I), fixed, permeabilized, labeled with anti-CLIC1 (B), anti-CLIC4 (F), anti-CLIC5 (J) antibodies and further stained with DAPI (C, G, K). D, H and L are merge images showing colocalization of CLIC1 and CLIC4 to the endoplasmic reticulum whereas CLIC5 shows much less colocalization. D', H' and L' are enlarged images of the squared region. (II) Bar graph representing percentage colocalization of CLIC1, CLIC4 and CLIC5 to endoplasmic reticulum (n=3).