Insulin Antibody MAB1417 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemInsulin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMAB1417
ApplicationsImmunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
ReactivityHuman, Mouse, Bovine
TargetInsulin
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
IsotypeIgG2a
Clone182410
HostRat
NCBI Full Gene Nameinsulin
ClonalityMonoclonal
NCBI Gene AliasesIDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant human Insulin
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects bovine, human, and mouse insulin

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Ciliary ARL13B prevents obesity in micebioRxiv : the preprint server for biologyAugust 4, 2023Terry TT, Gigante ED, Alexandre CM, Brewer KM, Engle SE, Yue X, Berbari NF, Vaisse C, Caspary T.Sigma, T7451); chicken anti-ACIII (1:1000, Encor, CPCA-ACIII); rat anti-insulin (1:1000, R&D systems, MAB1417); rabbit anti-glucagon (1:2000, Abcam, ab92517). Secondary antibodies used were goat anti-mouse AlexaFluor

(2) STAT3 suppression and β-cell ablation enhance α-to-β reprogramming mediated by Pdx1Scientific reportsDecember 10, 2022Wakabayashi Y, Miyatsuka T, Miura M, Himuro M, Taguchi T, Iida H, Nishida Y, Fujitani Y, Watada H.

(3) Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolismNature biomedical engineeringNovember 1, 2022Jimenez-Gonzalez M, Li R, Pomeranz LE, Alvarsson A, Marongiu R, Hampton RF, Kaplitt MG, Vasavada RC, Schwartz GJ, Stanley SA.1:50 dilution; mCherry (Abcam, ab205402), synapsin1 (Cell Signaling, 5297) and insulin (R&D systems MAB1417) at 1:1,000 dilution. Secondary antibodies used were Alexa Fluor 647 anti-rabbit, Alexa Fluor 546 anti-rabbit,

(4) Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic β-Cell Function by Preventing Dedifferentiation and ApoptosisFrontiers in pharmacologyJanuary 1, 2022Lei L, Huan Y, Liu Q, Li C, Cao H, Ji W, Gao X, Fu Y, Li P, Zhang R, et al.rabbit anti-ALDH1A3 antibody (1:100, NBP2-15339, NOVUS, USA), and rat anti-insulin antibody (1:200, MAB1417, R&D Systems, USA). The secondary antibodies were Alexa Fluor 488 donkey anti-rabbit IgG (1:200, A-21206,

(5) Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell functionScience advancesDecember 17, 2021Brown MR, Sen SK, Mazzone A, Her TK, Xiong Y, Lee JH, Javeed N, Colwell CS, Rakshit K, LeBrasseur NK, et al.with 4% formaldehyde for 10 min at room temperature. The cells were then immunostained for insulin (MAB1417, R&D Systems, Minneapolis, MN), FOXO1 (C29H4, Cell Signaling Technologies), and/or DBP (12662-1-AP,

(6) Tet2 Controls the Responses of β cells to Inflammation in Autoimmune DiabetesNature communicationsAugust 20, 2021Rui J, Deng S, Perdigoto AL, Ponath G, Kursawe R, Lawlor N, Sumida T, Levine-Ritterman M, Stitzel ML, Pitt D, et al.

(7) Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature SlowlyDiabetesMay 1, 2021Sharon Lee, Jing Zhang, Supraja Saravanakumar, Marcus F. Flisher, David R. Grimm, Talitha van der Meulen, Mark O. Huising1. Primary antibodies include guinea pig anti-insulin (cat. no. A0564, 1:500; Dako), rat anti-insulin (MAB1417, 1:500; R&D Systems), guinea pig anti-Ucn3 (044, 1:1,000) and rabbit anti-Ucn3 (7218, 1:1,000) (gifts

(8) Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic SteatohepatitisInternational journal of molecular sciencesAugust 10, 2020Kojima M, Takahashi H, Kuwashiro T, Tanaka K, Mori H, Ozaki I, Kitajima Y, Matsuda Y, Ashida K, Eguchi Y, et al.International, Temecula, CA, USA) to confirm the tumor lesion as HCC [55,56] and anti-insulin antibodies (Cat# MAB1417, R&D Systems, Minneapolis, MN, USA) to identify pancreatic β-cells. The substrate reaction, after incubation

(9) Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetesScientific reportsMarch 9, 2020Elizondo DM, Brandy NZD, da Silva RLL, de Moura TR, Ali J, Yang D, Lipscomb MW.solution and blocked with 0.2% BSA in permeabilization buffer. Sections were stained with insulin (cat# MAB1417; R&D Systems), CD31 (clone MEC 13.3), IFNγ (clone XMG1.2) and/or CD45 (clone 30-F11), followed by extensive

(10) Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy™ (ADi™), ADi-100BiomedicinesMarch 4, 2020Alleva DG, Rezaee M, Yip L, Ren G, Rosenberg J, Concepcion W, Escher A, Shabahang S, Thakor AS.Torrance, CA, USA) or paraffin, and stained for insulin using a rat anti-insulin primary antibody ((1:100, #MAB1417, R & D systems, Minneapolis, MN, USA) and a donkey anti-rat IgG secondary antibody conjugated to Alexa488

(11) VEGF-B ablation in pancreatic β-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptakeScientific reportsJanuary 22, 2020Ning FC, Jensen N, Mi J, Lindström W, Balan M, Muhl L, Eriksson U, Nilsson I, Nyqvist D.

(12) Stachyose Improves the Effects of Berberine on Glucose Metabolism by Regulating Intestinal Microbiota and Short-Chain Fatty Acids in Spontaneous Type 2 Diabetic KKAy MiceFrontiers in pharmacologyJanuary 1, 2020Cao H, Li C, Lei L, Wang X, Liu S, Liu Q, Huan Y, Sun S, Shen Z.paraffin, and dissected to prepare 5-μm-thick slides, then stained with antibodies against insulin (MAB1417; R&D Systems, USA) and glucagon (ab92517; Abcam, USA) as previously described (Li et al., 2017). The

(13) SHBG141-161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell linesScientific reportsDecember 19, 2019De Toni L, Guidolin D, De Filippis V, Peterle D, Rocca MS, Di Nisio A, De Rocco Ponce M, Foresta C.anti-mouse GPRC6A IgG and with rat-anti human/mouse insulin IgG (dilution 1:200; R&D System; RRID: MAB1417) that required appropriate secondary reagent (FITC conjugated-anti mouse IgG; dilution 1:100; Santa Cruz Biotechnology Inc

(14) Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key β-Cell-Specific Disease GenesCell reportsMarch 12, 2019Fang Z, Weng C, Li H, Tao R, Mai W, Liu X, Lu L, Lai S, Duan Q, Alvarez C, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rat monoclonal Insulin (Clone 182410),R&D Systems,Cat# MAB1417; RRID:AB_2126533 Anti-Scc4 antibody [EPR14390] (Mau2) 100ul,Abcam,Cat# ab183033; RRID:AB_2783830 Rabbit

(15) The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell massEJNMMI ResearchDecember 27, 2018Mahabuba Jahan et al.(HPA014259, dilution 1:10, Atlas Antibodies, Stockholm, Sweden), as well as with rat anti-human insulin (MAB1417, dilution 1:100, R&D Systems, Minneapolis, MN, USA) and Alexa-647-conjugated mouse anti-glucagon (G2654,

(16) Perinatal exposure to high dietary advanced glycation end products in transgenic NOD8.3 mice leads to pancreatic beta cell dysfunctionIsletsJanuary 2, 2018Danielle J. Borg et al.donkey serum (S30-100; Merck Millipore), and stained with either primary antibodies (rat anti-insulin (MAB1417; R&D Systems); mouse anti-proinsulin (MAB13361; R&D Systems); rabbit anti-glucagon (A0565; DAKO); goat

(17) Generation of Islet-like Cell Aggregates from Human Adipose Tissue-derived Stem Cells by Lentiviral Overexpression of PDX-1Int J Organ Transplant MedJune 18, 2015M. Bahrebar, M. Soleimani, M. H. Karimi, A. Vahdati, R. Yaghobitemperature then removed it. The cells were stained by rat anti-human insulin antibody (1:200) (R&D systems, MAB1417, USA) and diluted in BSA/PBS 0.2% at 4 °C for overnight. The cells were washed 3 times with PBS-Tween

(18) Macrophage contact dependent and independent TLR4 mechanisms induce β-cell dysfunction and apoptosis in a mouse model of type 2 diabetesPloS oneJanuary 1, 2014Cucak H, Mayer C, Tonnesen M, Thomsen LH, Grunnet LG, Rosendahl A.

(19) Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarizationJournal of leukocyte biologyJanuary 1, 2014Cucak H, Grunnet LG, Rosendahl A.

(20) Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetesNatureOctober 18, 2012Hagberg CE, Mehlem A, Falkevall A, Muhl L, Fam BC, Ortsäter H, Scotney P, Nyqvist D, Samén E, Lu L, et al.

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Insulin Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Insulin Antibody from R&D Systems, a Bio-Techne Brand

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