Fig 1: Fluorescent images of the resected tumor tissues: (A) the immuno-fluorescent intensities of CD133 (green) and Nanog (red); (B) the immuno-fluorescent Sox2 (green) and Nanog (red) images; and (C) the immuno-fluorescent ABCG2 (green) and Nanog (red) images. The spheroid tumor sections from mice fed with 2 and 4 g/kg of BJ and water control were incubated with primary antibodies followed by FITC- or TRITC-conjugated secondary antibodies, before being counter-stained with DAPI (blue). Images taken from confocal microscopy were merged (scale bar, 50 µm).