IL-15R alpha Antibody AF247 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-15R alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF247
NCBI Gene AliasesCD215
ApplicationsWestern Blot, Flow Cytometry, Immunohistochemistry, Neutralization, Immunocytochemistry, CyTOF-ready
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
TargetIL-15R alpha
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
IsotypeIgG
ReactivityHuman
NCBI Full Gene Nameinterleukin 15 receptor subunit alpha
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human, IL-15 R alpha, Ile31-Thr172, Accession # EAW86418
ConcentrationLYOPH
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human IL-2 R gamma is observed

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Complement-activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cellsThe Journal of clinical investigationJuly 1, 2020Xie CB, Jiang B, Qin L, Tellides G, Kirkiles-Smith NC, Jane-Wit D, Pober JS.overnight at 4°C. Antibodies included: IL-15 (Santa Cruz Biotechnology, sc-8437), IL-15Rα (R&D Systems, AF247), Hsp90α/β (Santa Cruz Technology, sc-13119), calnexin (Cell Signaling Technology, 2679), lamin A/C (Santa Cruz

(2) IL-15Rα membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signalingJournal of Cell ScienceMarch 1, 2020Agnès Quéméner et al.soluble human IL-2Rβ protein (224-2B) was purchased from R&D Systems. Polyclonal goat anti-human IL-15Rα (AF247, used at a 1:100 dilution), polyclonal goat isotype control IgG (AB-108-C, used at a 1:1000 dilution) PE-conjugated

(3) Lymphocyte‑derived microparticles stimulate osteoclastogenesis by inducing RANKL in fibroblasts of odontogenic keratocystsOncology reportsDecember 1, 2018Man QW, Zhang LZ, Zhao Y, Liu JY, Zheng YY, Zhao YF, Liu B.Jurkat cell‑derived MPs (5 or 10 µg/ml) or with 100 ng/ml anti‑human IL‑15Rα antibody (goat; cat. no. AF247; R&D Systems, Inc., Minneapolis, MN, USA) for 72 h at 37˚C. For co‑culture with Jurkat cells, OKC fibroblasts

(4) Efficient production and enhanced tumor delivery of engineered extracellular vesiclesBiomaterialsOctober 1, 2016Watson DC, Bayik D, Srivatsan A, Bergamaschi C, Valentin A, Niu G, Bear J, Monninger M, Sun M, Morales-Kastresana A, et al.CA), αAlix (clone 3A9, LifeSpan Biosciences; Seattle, WA), αIL-15 (AF315, R&D Systems) and αIL-15Rα (AF247, R&D Systems). All blots were probed overnight at 4°C. Detection of EV-associated IL-15 was performed

(5) Recombinant human heterodimeric IL-15 complex displays extensive and reproducible N- and O-linked glycosylationGlycoconjugate JournalJune 1, 2016M. Thaysen-Andersen et al.Western immunoblotting using anti-human IL-15 or anti-human IL-15Rα polyclonal goat IgG antibodies (product #AF315 and #AF247, respectively, R&D Systems). Release and handling of N- and O-glycans of human IL-15 and sIL-15Rα The

(6) Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patientsBMC Musculoskeletal DisordersDecember 1, 2015Alicia Santos Savio, Ana Cecilia Machado Diaz, Araceli Chico Capote, Jamilet Miranda Navarro, Yunier Rodríguez Alvarez, Ricardo Bringas Pérez, Miguel Estévez del Toro, Gerardo E Guillen NietoPBS containing 0.05% Tween 20. IL-15Ralpha was detected with a specific antibody against IL-15Ralpha (AF247, R&D System). The bound IL-15Ralpha was detected with HRP-conjugated goat antihuman IgG (A0170, Sigma,

(7) Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1β, IFN-β, and IL-15 PathwaysJournal of immunology (Baltimore, Md. : 1950)September 15, 2015Mattiola I, Pesant M, Tentorio PF, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D.

(8) Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cellsGene therapyJanuary 1, 2015Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai NY, Valentin A, Felber BK, et al.IL-15Rα expression was analyzed by western immunoblot using polyclonal goat anti-human IL-15Rα antibody AF247 (R&D Systems). For western immunoblot assays, aliquots (1/200) from the extracellular fractions were

(9) IL-12-producing monocytes and HLA-E control HCMV-driven NKG2C+ NK cell expansionThe Journal of clinical investigationDecember 1, 2014Rölle A, Pollmann J, Ewen EM, Le VT, Halenius A, Hengel H, Cerwenka A.antibody (clone 125-2H, MBL International), 5 μg/ml α–IL-15R antibody (Purified Polyclonal Goat Ab, #AF247, R&D Systems), 10–20 μg/ml α–IL-2 antibody (clone MQ1-17H12, BioLegend), 4 μg/ml F(ab)2 fragments of

(10) Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemiaBloodMay 15, 2014Williams MT, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman KE, McAndrew R, Thomas A, Spence A, et al.

(11) Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Rα and NKG2D triggeringCancer researchJanuary 1, 2014Romero AI, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, et al.

(12) Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Rα in human monocytes and dendritic cellsThe Journal of biological chemistryNovember 23, 2012Müller JR, Waldmann TA, Kruhlak MJ, Dubois S.

(13) Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid ArthritisArthritisJuly 25, 2012Ana Cecilia Machado Diaz, Araceli Chico Capote, Celia Aurora Arrieta Aguero, Yunier Rodríguez Alvarez, Diana García del Barco Herrera, Miguel Estévez del Toro, Gerardo E. Guillen Nieto, Alicia Santos Saviowashes with PBS containing 0.05% Tween 20. IL-15Rα was detected with specific antibody against IL-15Rα (AF247, R&D System). The bound IL15Rα was detected with HRP-conjugated goat antihuman IgG (A0170, Sigma, USA)

(14) The differential production of cytokines by human Langerhans cells and dermal CD14(+) DCs controls CTL primingBloodJune 14, 2012Jacques Banchereau, LuAnn Thompson-Snipes, Sandra Zurawski, Jean-Philippe Blanck, Yanying Cao, Sandra Clayton, Jean-Pierre Gorvel, Gerard Zurawski, Eynav Klechevskyindicated. A combination of anti–IL-15 (Clone MAB 647; R&D Systems) together with anti–IL-15R␣ (Clone AF247; R&D Systems) was also used as indicated. For allogeneic primary responses, 1 ⫻ 105 CFSElabeled naive

(15) Increased serum soluble IL-15Rα levels in T-cell large granular lymphocyte leukemiaBloodJanuary 5, 2012Jing Chen, Mike Petrus, Richard Bamford, Joanna H. Shih, John C. Morris, John E. Janik, Thomas A. Waldmannthe assay, a high absorption plate was coated with the polyclonal goat anti–human IL-15R␣ antibody AF247 (R&D Systems). After blocking, samples were added to the plate to allow sIL-15R␣ to bind to the plate.

(16) Interleukin-15 plays a central role in human kidney physiology and cancer through the γc signaling pathwayPloS oneJanuary 1, 2012Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, François H, Lecru L, et al.phosphorylated STAT5 (3939) were obtained from Cell Signaling (Beverly, MA). Antibodies against IL-15Rα (AF247), E-cadherin (AF648) and PE-conjugated anti-E-cadherin (FAB18381P) were obtained from R&D Systems Europe

(17) EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15BloodMay 7, 2009Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C.

(18) Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infectionJournal of immunology (Baltimore, Md. : 1950)February 1, 2009Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M Jr, Lifson JD, Katsikis PD, et al.

(19) Genes regulated by interferon-γ in human uterine microvascular endothelial cellsInternational Journal of Molecular MedicineNovember 1, 2007Hideo Honjo, Shinji Fushiki, Takeshi Yamaguchi, Tadahiro Yasuo, Kotaro KitayaSanta Cruz Biotechnology. Goat antihuman interleukin-15 receptor α chain (IL15RA) polyclonal antibody (AF247) and recombinant human IL-15 receptor α chain/Fc chimera (147-IR, extracellular domain of IL-15 receptor

(20) Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loopsJournal of immunology (Baltimore, Md. : 1950)September 15, 2005Miranda-Carús ME, Benito-Miguel M, Llamas MA, Balsa A, Martín-Mola E.

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IL-15R alpha Antibody

IL-15R alpha Antibody

IL-15R alpha Antibody

IL-15R alpha Antibody

IL-15R alpha Antibody

IL-15R alpha Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (26)

Reviews

IL-15R alpha Antibody from R&D Systems, a Bio-Techne Brand

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