Fig 2: The BMP pathway is activated in bone marrow (BM) and peripheral blood of AML patients.a BMP2 and BMP4 concentrations in bone marrow (BM) supernatants from AML patients (n = 17) and healthy donors (n = 19) evaluated by ELISA. b BMP2 and BMP4 mRNA level in blood and bone marrow MNCs from healthy donors and AML patients’ samples at diagnosis. c BMP2 and BMP4 mRNA level in bone marrow MNCs only, from healthy donors and AML patients’ samples at diagnosis. d Expression of BMP receptors: BMPR1A, BMPR1B and BMPR2, and BMP target genes: Id1 and RunX1 evaluated in normal (n = 14–25) and AML (n = 34–51) grouped BM and blood samples by RT-qPCR. Arbitrary unit (AU): relative expression compared to a healthy sample used as a reference for each PCR experiment. P values were determined using the Mann-Whitney U test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

Fig 3: The presence of BMPR1A at the cell surface of AML samples is induced by BMP4 and BMPR1A and is associated with the presence of very immature cells.a Percentage of mononuclear cells (MNCs) of healthy donors (n = 8) and AML (n = 11) samples expressing BMPR1A at their membrane determined by flow cytometry. b Correlation between the expressions of BMPR1A and Id1 in AML samples at diagnosis (n = 50). c Relative expression of BMPR1A in MNCs from healthy donors (n = 6) and AML patients (n = 9), exposed or not, to BMP4 (10 ng/mL) for 24 h. d Effect of BMP4 (10 ng/mL) exposure on the proportion of BMPR1A-positive cells at the surface in healthy and AML MNC samples (n = 6 patients for each cohort). e Effect of BMP4 (10 ng/mL) exposure for 24 h on the proportion of very immature leukemic cells in AML mononuclear cells (MNCs), evaluated by long-term culture-initiating cells (LTC-IC) assay (n = 3). f Flow cytometric analysis of the expression of BMPR1A at the cell surface according to CD34 and CD38 status of MNCs from an AML patient. g Number of colony-forming cells (CFC) and LTC-IC among peripheral blood MNCs from AML patients according to their low (n = 8) or high (n = 6) BMPR1A mRNA level. h AML samples with low (n = 6) or high (n = 7) BMPR1A mRNA levels were analyzed for ALDH activity by flow cytometry. NT non-treated, NBM normal bone marrow. Statistical analysis: P values were determined using Spearman’s nonparametric test (b); Wilcoxon matched-pairs signed rank test (c, d); *P < 0.05; **P < 0.01

Fig 4: BMPR1A, ?Np73, and NANOG expression at diagnosis are correlated with patient outcome.Percentage of AML patients in remission or relapse 3 years after diagnosis, according to BMPR1A, ?Np73 or NANOG expression levels separately (n = 19) (a) or in combination (n = 13) (b). c Overall survival (OS) curve of AML patients from the date of sampling onwards, by using the Kaplan-Meier method. The level of significance was determined by the log-rank test (n = 52; 23 high and 29 low NANOG level). d Overall survival (OS) curve of AML patients from the date of sampling onwards, by using the Kaplan-Meier method. TCGA dataset from AML patients of the intermediate group. e Schematic representation of the role played by BMPs on homeostasis under healthy conditions (top) and the cascade of dysregulations associated with increased concentration of BMP4 in the microenvironment of leukemic immature cells (bottom)

Fig 5: ?Np73 overexpression in AML samples at diagnosis is correlated with BMP pathway activation and is associated with the presence of very immature cells.a Expression of TAp73 and ?Np73 in mononuclear cell (MNC) samples from healthy donors (n = 19–29) and AML at diagnosis (n = 46–54) quantified by RT-qPCR. Arbitrary Unit (AU), relative expression compared to a healthy sample used as a reference. b Correlations between the expressions of ?Np73 and BMPR1A (n = 51). P values were determined using Spearman’s nonparametric test. c Relative expression of ?Np73 in MNCs from healthy donors (n = 5) and AML patients (n = 7), exposed or not, to BMP4 (10 ng/mL) for 24 h; NT not treated. d Western blot analysis of AML cells exposed or not, to BMP4 (20 ng/mL) for 24 h (n = 2); N not treated. e Relative expression of ?Np73 in KG1A cells exposed to BMP4 (10 ng/mL) for 24 h with (n = 8). f Expression of ?Np73 in KG1A cells exposed to BMP4, in presence of either an antibody specific for BMPR1A or a control antibody, measured by RT-qPCR. Ratio was calculated according to untreated KG1A cells. g CFC and LTC-IC values in AML samples according to low (<8 AU, n = 6) or high (>8 AU, n = 9) ?Np73 levels. h Flow cytometry analysis of ALDH activity in AML samples with low (n = 7) or high (n = 9) ?Np73 expressions levels. Statistical analysis: Wilcoxon matched-pairs signed rank test was used for (c, e, f); *P < 0.05; **P < 0.01; ***P < 0.001