Fig 1: Comparing somatotropic hormones, adult body size and glucose homeostasis between wild-type (WT) B6 and 129S2 mice. (A) Plasma IGF-I concentration. ***P < 0.001, Student’s t-test; N = 18–19 per group. (B) Body length (naso-anal distance). (C) Body weight. N = 11–13 per group. (D) Plasma acid-labile subunit (ALS). ***P < 0.001, Student’s t-test; N = 9 per group. (E) Comparing plasma GH concentration by rank plot analysis (Xu et al., 2011) between WT females from B6 (open marks) and 129S2 (black marks) background (P = 0.012, log-rank test). (F) Glucose tolerance test (GTT) in male and female mice. Glucose (2 g kg-1 body weight) was intraperitoneally (i.p.) injected at T0 and glycemia measured at T0, 15, 30, 60, and 120 min. Note that all groups rapidly regained control over glycemia. ***P < 0.002 (N = 7–8 per group) comparing B6 with 129S2. Individual area under curve (AUC) between both strains is significantly different (P < 0.001, one-way ANOVA with Bonferroni’s post hoc test). (G) Glycemia in the fed state. ***P < 0.001 (N = 6–10 per group) (one-way ANOVA with Bonferroni’s post hoc test). A-E and G were performed in 11- to 13-week-old mice. Tests in panel F were performed in 2-month-old mice. Error bars indicate SEM.