Fig 2: The role of the SVZ/WM area in myeloid cell proliferation and migration signaling during early WM repopulation.(A–D) Transcriptional analysis of SVZ tissue during early WM repopulation. (A) Bulk tissue RNA-seq analysis was performed on micro-dissected SVZ tissue from control, 14 day PLX3397, and 5 day recovery brains (n=5). (B) Heatmap of DEGs between 14 day PLX3397 (Elim) and 5 day recovery SVZ tissue. Gene expression data can be explored at http://rnaseq.mind.uci.edu/green/alt_repop_svz/gene_search.php. (C) Gene ontology chord plot of DEGs between control and 5 day SVZ tissue. (D) Plot highlighting expression (% of control) changes in myeloid-associated genes in depleted (14d PLX3397) and repopulated (5d recovery) SVZ tissue. (E–H) Neutralization of CCL12 during WM repopulation. (E) Experiment schematic of CCL12 neutralization study: 2-month-old WT mice were treated for 14 days with PLX3397 (600 ppm) and then placed on control diet for 7 days allowing for WM repopulation. Four i.p. injections were administered of anti-CCL12 antibody or goat IgG (Isotype control) at 1 day recovery, 3 day recovery, 5 day recovery, and 6 day recovery. (F) Representative whole brain images of IBA1+ cell (green) deposition following treatment. (G–H) Quantification of number of total IBA1+ cells and total distance traveled by IBA1+ wavefront in (F). Total distance was calculated by measuring the length from the ventricular edge of SVZ to the leading edge of the IBA1+ cell wavefront. Figure 6—source data 1.The role of the SVZ/WM area in myeloid cell proliferation and migration signaling during early WM repopulation.