beta-Catenin Antibody AF1329 from R&D Systems, a Bio-Techne Brand

Product Specs
Itembeta-Catenin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF1329
NCBI Gene AliasesCTNNB, EVR7, NEDSDV, MRD19, armadillo
ApplicationsWestern Blot, Simple Western, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, CyTOF-ready
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Targetbeta-Catenin
IsotypeIgG
HostGoat
ReactivityHuman, Mouse, Rat
NCBI Full Gene Namecatenin beta 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human beta-Catenin, Ala2-Leu781, Accession # P35222
ConcentrationLYOPH
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human, mouse and rat beta-Catenin in Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) GSK3β Inhibition Ameliorates Atherosclerotic CalcificationInternational journal of molecular sciencesJuly 19, 2023Cai X, Zhao Y, Yang Y, Wu X, Zhang L, Ma JA, Ji J, Boström KI, Yao Y.(Cell Signaling Technology, Danvers, MA, USA, 9743, 93115), β-catenin (R&D System, Minneapolis, MN, USA, AF1329), and osterix (Santa Cruz Biotechnology, Dallas, TX, USA, sc-22536) were used. β-Actin (Sigma-Aldrich,

(2) GSK3β Inhibition Reduced Vascular Calcification in Ins2Akita/+ MiceInternational Journal of Molecular SciencesMarch 22, 2023Kristina I. Boström, Xiaojing Qiao, Yan Zhao, Xiuju Wu, Li Zhang, Jocelyn A. Ma, Jaden Ji, Xinjiang Cai, Yucheng Yaotissue lysates were used. These include SMAD1 (Cell Signaling Technology, 9743), β-catenin (R&D system, AF1329. Minneapolis, MN. USA), osterix (Santa Cruz Biotechnology, sc-22536. Dallas, TX, USA), Flk1 and VE-cadherin

(3) TCF/LEF regulation of the topologically associated domain ADI promotes mESCs to exit the pluripotent ground stateCell reportsSeptember 14, 2021Doumpas N, Söderholm S, Narula S, Moreira S, Doble BW, Cantù C, Basler K.

(4) Shifting osteogenesis in vascular calcificationJCI insightMay 24, 2021Yao J, Wu X, Qiao X, Zhang D, Zhang L, Ma JA, Cai X, Boström KI, Yao Y.from Cell Signaling Technology, 9743, 433T, and 93115); β-catenin and Cbfa1 (all from R&D Systems, AF1329 and MAB2006); osterix (Santa Cruz Biotechnology, sc-22536); Flk1 and VE-cadherin (all from BD Biosciences,

(5) LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5pEuropean review for medical and pharmacological sciencesJuly 1, 2020Liu XF, Wang K, Du HC.

(6) The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-ActinCellsMarch 19, 2020Yamazaki S, Gerhold C, Yamamoto K, Ueno Y, Grosse R, Miyamoto K, Harata M.chromatin fraction in the sonicated nuclear lysates was immunoprecipitated with anti-β-catenin antibody (AF1329, R&D Systems, Minneapolis, MN, USA) bound to Dynabeads Protein G (Invitrogen, Carlsbad, CA, USA). Purified

(7) Dopamine D₂ receptor modulates Wnt expression and control of cell proliferationScientific reportsNovember 14, 2019Han F, Konkalmatt P, Mokashi C, Kumar M, Zhang Y, Ko A, Farino ZJ, Asico LD, Xu G, Gildea J, et al.(catalog#ab15580, Abcam). We also stained for β-catenin using a goat polyclonal anti-β-catenin antibody (catalog#AF1329, R&D Systems, Minneapolis, MN). DAPI was used to visualize the nuclei of individual cells. Secondary

(8) Wnt/β-catenin links oxidative stress to podocyte injury and proteinuriaKidney internationalApril 1, 2019Zhou L, Chen X, Lu M, Wu Q, Yuan Q, Hu C, Miao J, Zhang Y, Li H, Hou FF, et al.sections were stained with mouse monoclonal anti-AOPPs (Clone 3F2) and rabbit monoclonal anti-β-catenin (AF1329, R&D Systems), respectively. The anti-AOPPs monoclonal antibody was described previously and demonstrated

(9) Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll‑like receptor‑4‑NADPH oxidase 4 signaling pathwayMolecular medicine reportsJanuary 1, 2019You ZP, Yu MJ, Zhang YL, Shi K.

(10) Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β‑catenin pathwayMolecular medicine reportsDecember 1, 2018Zeng S, Zhu B, Zeng J, Wu W, Jiang C.1:600), anti-wnt5a (Abcam; cat. no. ab174963; 1:1,000), anti-β-catenin (R&D Systems, Inc.; cat. no. AF1329; 1:1,000), anti-cyclin D1 (R&D Systems, Inc.; cat. no. MAB4314; 1:900) and anti-β-actin (Abcam; cat.

(11) Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cellsScientific reportsFebruary 5, 2018Udagawa T, Harita Y, Miura K, Mitsui J, Ode KL, Morishita S, Urae S, Kanda S, Kajiho Y, Tsurumi H, et al.

(12) Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatasesThe Journal of experimental medicineSeptember 4, 2017Luo M, Flood EC, Almeida D, Yan L, Berlin DA, Heerdt PM, Hajjar KA.Biotechnology, Inc.), anti-ANXA4 (NBP1-90151; Novus Biologicals), anti-SHP2 (610621; BD), and anti–β catenin (AF1329; R&D Systems). For immunoblotting of extravascular lung albumin, total protein from highly perfused

(13) WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiationNature communicationsJune 7, 2017Xu M, Horrell J, Snitow M, Cui J, Gochnauer H, Syrett CM, Kallish S, Seykora JT, Liu F, Gaillard D, et al.

(14) Dchs1-Fat4 regulation of polarized cell behaviours during skeletal morphogenesisNature communicationsMay 5, 2016Mao Y, Kuta A, Crespo-Enriquez I, Whiting D, Martin T, Mulvaney J, Irvine KD, Francis-West P.

(15) Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased β -catenin inhibition and increased Wnt5a/b interactionPhysiological ReportsMarch 22, 2016Kristina Rydell-Törmänen, Xiao-Hong Zhou, Oskar Hallgren, Jonas Einarsson, Leif Eriksson, Annika Andersson-Sjöland, Gunilla Westergren-ThorssonEMD Millipore Corp. Billerica, MA), Wnt5a/b (1:100, ab72583, Abcam, Cambridge, UK), β‐catenin (1:100, AF1329, R&D Systems, Minneapolis, MN), Disheveled‐associated activator of morphogenesis 1/DAAM1 (1:200, ab71327,

(16) ROS-induced endothelial stress contributes to pulmonary fibrosis through pericytes and Wnt signalingLaboratory investigation; a journal of technical methods and pathologyFebruary 1, 2016Andersson-Sjöland A, Karlsson JC, Rydell-Törmänen K.Billerica, MA, USA), Wnt3a (1:300, 09-162, EMD Millipore), Wnt5a (1:100, ab72583, Abcam), β-catenin (1:100, AF1329, R&D Systems, Minneapolis, MN, USA), CD31 (1:100, ab56299, Abcam), Vimentin (1:600, ab92547, Abcam),

(17) Interlocked positive and negative feedback network motifs regulate β-catenin activity in the adherens junction pathwayMolecular Biology of the CellNovember 5, 2015David J. Klinke, Nicholas Horvath, Vanessa Cuppett, Yueting Wu, Wentao Deng, Rania Kanj(Grand Island, NY). A WISP-1 DuoSet ELISA (DY1627) and polyclonal goat anti–β-catenin IgG antibody (AF1329) were purchased from R&D Systems (Minneapolis, MN). A rat monoclonal IgG1 anti–E-cadherin (clone: DECMA-1)

(18) Fat4/Dchs1 signaling between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branchingDevelopment (Cambridge, England)August 1, 2015Mao Y, Francis-West P, Irvine KD.described previously (Mao et al., 2011), using the following primary antibodies: goat anti-β-catenin (AF1329, R&D Systems; 1:100), goat anti-E-cadherin (AF748, R&D Systems; 1:400), rabbit anti-Six2 (11562-1AP,

(19) WNT1-induced Secreted Protein-1 (WISP1), a Novel Regulator of Bone Turnover and Wnt SignalingThe Journal of biological chemistryMay 29, 2015Maeda A, Ono M, Holmbeck K, Li L, Kilts TM, Kram V, Noonan ML, Yoshioka Y, McNerny EM, Tantillo MA, et al.

(20) Integrin β1 controls VE-cadherin localization and blood vessel stabilityNature communicationsMarch 10, 2015Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams RH.

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beta-Catenin Antibody

beta-Catenin Antibody

beta-Catenin Antibody

beta-Catenin Antibody

beta-Catenin Antibody

beta-Catenin Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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beta-Catenin Antibody from R&D Systems, a Bio-Techne Brand

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