Fig 1: A?B transcytosis of Chx domain I induces a redistribution of LMAN1 following intraluminal injection (ILI) in vivo. Co-localization of LMAN1 with (a) COPI or (b) COPII in untreated enterocytes prior to and 15-min post-ILI of Chx266-hGH. Co-localization of LMAN1 with (c) COPI or (d) at 15-min post-ILI. (e) ELISA-based binding interactions between LMAN1 with Chx266-hGH compared to hGH (N = 3; mean ± SE). (f) The extent of in vitro A?B transcytosis of Chx266-hGH across SMI-100 tissues after 60 min in the presence and absence of an antibody recognizing LMAN1 (N = 3; mean ± SE). Intracellular localization of Ribophorin in enterocytes (g) before treatment or (h) 15-min post-ILI of Chx266-hGH. Arrow = apical epithelial surface; dashed line = epithelial cell-basement membrane demarcation; G = goblet cell; l-p = lamina propria. Nuclei stained with 4',6-diamidino-2-phenylindole (DAPI; blue).