Fig 1: Increased Dectin-2 expression in diabetic patients’ PBMCs. Flow cytometric analysis was conducted to identify the expression levels of Dectin-2 on different leukocyte subsets in PBMCs isolated from non-diabetic and diabetic patients. (A) Representative dot plot identifying three leukocyte subsets. (B) Representative dot plot of Dectin-2 expression in different leukocyte subsets. (C) Bar graph analysis of Dectin-2 expression calculated as MFI with representative histogram. (D) Representative density plot analysis of CD45+CD14+Dectin-2+ population in PBMCs isolated from non-diabetic and diabetic patients. (E) The percentage of CD45+CD14+Dectin-2+ population in PBMCs isolated from non-diabetic and diabetic patients. (F) Bar graph analysis of CD45+CD14+Dectin-2+ expression calculated as MFI with representative histogram. Gray (non-stained), green (lymphocytes), blue (monocytes), and purple (neutrophils). Data are presented as mean ± SEM with a minimum of n = 3. Statistical significance was assessed by Student’s t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
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