Fig 1: EB-A inhibits mast cell activation and degranulation in vivo and in vitro. (A) Mast cells were revealed by toluidine blue staining and counted (at least three random fields per tissue section). The black arrow indicates the mast cells. (B,C) mMCP-1 and ß-MCT levels in serum and BALF were measured using ELISA. (D,E) TPSAB1 protein expression level was, respectively, measured by immunofluorescence staining and Western blotting. (F,G) Histamine and LTC4 levels in serum were measured using ELISA. (H) Neutral red staining solution was used to stain degranulation in RBL-2H3 cells. (I) The release of ß-Hexosaminidase (ß-Hex) from RBL-2H3 cells was detected by a substrate chromogenic assay. (J) The release of histamine from RBL-2H3 cells was detected by ELISA. All data are shown as mean ± SD. ## p < 0.01, ### p < 0.001, compared with the NC group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the OVA group.