Fig 2: Schematic diagram representing the lncRNA WDR11-AS1 and PABPC1 interaction controlling ECM synthesis in OA chondrocytes. Upregulation of PABPC1 interferes with lncRNA WDR11-AS1 regulation and enhances SOX9 mRNA decay, thereby reducing ECM synthesis.

Fig 3: The binding of lncRNA WDR11-AS1 to PABPC1 stabilizes SOX9 expression and induces ECM synthesis in chondrocytes. (A) Western blot analysis showed SOX9 and PABPC1 levels in primary chondrocytes reducing WDR11-AS1 with siRNA-mediated PABPC1 knockdown. (B) Western blot analysis showed SOX9 and PABPC1 levels in primary chondrocytes overexpressing WDR11-AS1 with siRNA-mediated PABPC1 knockdown. (C) Western blot analysis showed COLII levels in primary chondrocytes reducing WDR11-AS1 with siRNA-mediated PABPC1 knockdown. (D) Western blot analysis showed COLII levels in primary chondrocytes overexpressing WDR11-AS1 with siRNA-mediated PABPC1 knockdown. * p < 0.05, the group vs. the sh-NC+si-NC group; # p < 0.05, the two groups under the horizontal line are compared. The measurement data are expressed as the mean ± standard deviation, and were analyzed by Mann-Whitney U test. The experiments were repeated three times.

Fig 4: Prognostic value of the TIIC signature score in the LUAD tissue array. A. Representative images of CFL1, HSP90AA1, and PFN1 based on multiplex IF staining in the high TIIC signature score group. B. Representative images of CFL1, HSP90AA1, and PFN1 based on multiplex IF staining in the low TIIC signature score group. C. Representative images of CCDC85B, HMGB1, PABPC1, and RPS15 based on multiplex IF staining in the high TIIC signature score group. D. Representative images of CCDC85B, HMGB1, PABPC1, and RPS15 based on multiplex IF staining in the low TIIC signature score group. E. Kaplan–Meier survival curves of the TIIC signature score regarding OS. F. Time-dependent ROC curves of the TIIC signature score regarding 1-, 2-, 3-, 4- and 5-year OS. G. Circos plot of different clinical factors in two TIIC signature score groups.

Fig 5: Microenvironment effect on the polyribosome profileK562 cells were cultured in N, H, CO or NCO setup for 72 h.(A) Profile of absorbance monitored at ? = 254 nm during fractionation of sucrose gradient (from top 10% to bottom 50%). Representative image presented, n = 3.(B) The same volume of fractions used for analysis of PABPC1 protein by Western blotting in the fractions. Fractions denoted: R – RNA binding proteins complexes; S – small ribosomal subunit; M- large ribosomal subunit and monosome; P – polyribosomes.(C) Area under polyribosome fractions and small ribosome subunit peak (S, fr. 2) measured using ImageJ and presented as ratio; n = 4.(D) Level of AHA-AlexaFluor 488 labeled proteins in K562 cells from N, H, CO or NCO conditions measured by flow cytometry; n = 3. (C and D) Mean values from at least 3 experiments ± ME are presented; Student’s ttest to compare cells as indicated by line above bars; *p<0.05, **p<0.005, ***p<0.001, ns – not significant.