Fig 1: H2S levels in the ECM-infected brain. (A) Reverse transcription polymerase chain reaction results demonstrated that CBS mRNA levels gradually decreased at the transcriptional level in a time-dependent manner. (B) Representative western blot image of CBS protein expression levels. GAPDH served as an internal control. (C) The levels of H2S derived from the brain were measured by ELISA. Data are presented as the mean ± standard error of the mean, n=10. *P<0.05 and **P<0.01 vs. 0 D. 0 D, uninfected group; 2 D, infection group treated with saline for 2 days; 3 D, infection group treated with saline for 3 days; 6 D, infection group treated with saline for 6 days. ECM, experimental cerebral malaria; CBS, cystathionine-ß-synthase.