Fig 1: (A) Fibrosis score (left) and Masson’s trichrome stain (middle and right) allowing the identification of fibrosis (in green) and myocytes (in pink) in detrusor tissues from control/non-NDO, and patient with NDO naïve or with secondary failure to BoNT/A1. Scale bar is 100µm. (B–E) Staining levels (left) and immunochemistry staining (middle and right) of beta-3 tubulin (B), SV2C (C), SNAP25 N-ter (D) and CHRM2 (E) in detrusor tissues from control, and patient with NDO naïve or with secondary failure to BoNT/A1. Scale bar is 20 µm. The data are mean ± SEM of n = 5 to 8 detrusor sections from n = 5 to 8 different patients. Post-ANOVA all-pairs Tukey-Kramer or Steel-Dwass tests, * p < 0.05 versus control patients. All groups were compared pairwise, only significant difference was signaled, no * means NS (not significant).