PE Mouse IgG2b, kappa isotype Ctrl 400314 from BioLegend

Product Specs
ItemPE Mouse IgG2b, kappa isotype Ctrl
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number400314
Quantity100 tests
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
Conjugate/TagPE
HostMouse
IsotypeIgG2b, Kappa
CloneMPC-11
Formattest sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ClonalityMonoclonal

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Mitotic bookmarking by SWI/SNF subunitsNatureJune 1, 2023Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF, et al.PE anti-Histone H3 Phospho (Ser10) Antibody (BioLegend #650808) or PE Mouse IgG2b, κ Isotype Ctrl Antibody (BioLegend #400314) and incubated for 20 min in the dark at room temperature. Next, cells were washed twice with 2 ml of

(2) Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell ActivationInternational journal of biological sciencesJanuary 1, 2023Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.BioLegend, #400125), APC mouse IgG2a (1:200; BioLegend, #400219), PE mouse IgG2b (1:200; BioLegend, #400313), and FITC mouse IgG1 (1:200; BioLegend, #400104) as control staining, respectively. For analyzing EphA4

(3) Comprehensive Evaluation of Multiple Approaches Targeting ABCB1 to Resensitize Docetaxel-Resistant Prostate Cancer Cell LinesInternational Journal of Molecular SciencesDecember 30, 2022Dinah Linke, Lukas Donix, Claudia Peitzsch, Holger H. H. Erb, Anna Dubrovska, Manuel Pfeifer, Christian Thomas, Susanne Fuessel, Kati Erdmannclone 5D3, 5 µg/mL; Biolegend) or corresponding isotype controls (IgG2a: 400214, clone MOPC-173, IgG2b: 400314, clone MPC-11; Biolegend) conjugated with phycoerythrin (PE). After incubation for 30 min at 4 °C in

(4) Highly sensitive and non-disruptive detection of residual undifferentiated cells by measuring miRNAs in culture supernatantScientific ReportsDecember 1, 2022Kanako Masumoto, Yuki Aihara, Mao Miyagawa Kuroishi, Natsuki Maeda, Yumiko Sakai, Yuma Oka, Yusuke Takahashi, Kenta Oda, Masatoshi Yanagidanumber of Oct4-positive cells was determined using Amnis™ ImageStream™ X MkII (Luminex). PE Mouse IgG2b (400314, BioLegend) was used to set the gate. Hoechst stain was used to stain the nucleus. After seeding iPSCs

(5) In mouse chronic pancreatitis CD25(+)FOXP3(+) regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune responseNature communicationsAugust 3, 2022Glaubitz J, Wilden A, Golchert J, Homuth G, Völker U, Bröker BM, Thiele T, Lerch MM, Mayerle J, Aghdassi AA, et al.IgG1, Isotype Ctrl (dilution 1:50, 400417, BioLegend), PE Mouse IgG2b, Isotype Ctrl (dilution 1:50, 400313, BioLegend), PE Mouse IgG1, Isotype Ctrl (dilution 1:50, 551436, BD), APC Rat IgG2b, Isotype Ctrl (dilution

(6) Galectin-9 contributes to the pathogenesis of atopic dermatitis via T cell immunoglobulin mucin-3Frontiers in ImmunologyJuly 22, 2022Wenxing Su, Ji Zhang, Shun Yang, Minhui Tang, Yu Shen, Cuiping Liu, Jiang Ji, Marcus Maurer, Qingqing Jiao(400108, Clone: RTK2071, Biolegend), mouse IgG2a (400246, Clone: MOPC-173, Biolegend), and mouse IgG 2b (400314, Clone: MPC-11, Biolegend) were used as isotype controls. Cells were analyzed with a Coulter Epics XL

(7) A New Method of Bone Stromal Cell Characterization by Flow CytometryCurrent ProtocolsMarch 29, 2022Chirayu Patel, Lihong Shi, John F. Whitesides, Brittni M. Foster, Roberto J. Fajardo, Ellen E. Quillen, Bethany A. KerrOSTEOCLAST,MACROPHAGE/ OSTEOCLAST,MACROPHAGE/ OSTEOCLAST,MACROPHAGE/ OSTEOCLAST "PE Mouse IgG2b, κ",BioLegend,400314,N.A "FITC Rat IgG2b, κ",BioLegend,400634,AB_893662 "PerCP-Cy5.5 Mouse IgG 2b,k",BioLegend,400338,N.A. "APC

(8) Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1Proceedings of the National Academy of Sciences of the United States of AmericaFebruary 15, 2022Oreskovic E, Wheeler EC, Mengwasser KE, Fujimura E, Martin TD, Tothova Z, Elledge SJ.(BioLegend catalog no. 311404). Isotype controls used were mouse IgG2b, k-PE (BioLegend catalog no. 400313), and mouse IgG2a, k-FITC (BioLegend catalog no. 400208). Cells were washed twice after staining and

(9) Development of a Stable Peptide-Based PET Tracer for Detecting CD133-Expressing Cancer CellsACS OmegaJanuary 11, 2022Kuan Hu et al.CD133 Antibody (Rat IgG2a, λ, cat. 141204, CA, USA), PE Mouse IgG2b (κ Isotype Ctrl Antibody, cat. 400314, BioLegend, CA, USA), and PE anti-human IgG Fc Antibody (Rat IgG2a, κ, cat. 410708) were used for flow cytometry

(10) SOX10 requirement for melanoma tumor growth is due, in part, to immune-mediated effectsCell reportsDecember 7, 2021Rosenbaum SR, Tiago M, Caksa S, Capparelli C, Purwin TJ, Kumar G, Glasheen M, Pomante D, Kotas D, Chervoneva I, et al.control",BioLegend,MOPC-21; RRID:AB_893664 "Mouse IgG2b, κ isotype control",BioLegend,MPC-11; Catalog no. 400313 CD8α,BioXCell,53-6.72; RRID:AB_1107671 Rat IgG2a,BioXCell,2A3; RRID:AB_1107769 ,, "Chemicals, peptides,

(11) Transcriptional Reprogramming and Constitutive PD-L1 Expression in Melanoma Are Associated with Dedifferentiation and Activation of Interferon and Tumour Necrosis Factor Signalling PathwaysCancers (Basel)August 24, 2021Antonio Ahn, Euan J. Rodger, Jyoti Motwani, Gregory Gimenez, Peter A. Stockwell, Matthew Parry, Peter Hersey, Aniruddha Chatterjee, Michael R. Eccles(Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b, Biolegend, catalog#:400314) have a maximum excitation at 575 nm. No overlap in fluorescence emission was detected between the FVS450

(12) KPNB1 Inhibitor Importazole Reduces Ionizing Radiation-Increased Cell Surface PD-L1 Expression by Modulating Expression and Nuclear Import of IRF1Current Issues in Molecular BiologyMay 19, 2021Hironori Yoshino, Yoshiaki Sato, Manabu Nakano(PE)-labeled anti-human PD-L1 monoclonal antibody (#329706) and PE-conjugated anti-mouse IgG2b antibody (#400314) were purchased from BioLegend (San Diego, CA, USA). Ambion Silencer® Select Pre-designed small interfering

(13) Inflamed Ulcerative Colitis Regions Associated With MRGPRX2-Mediated Mast Cell Degranulation and Cell Activation Modules, Defining a New Therapeutic TargetGastroenterologyApril 1, 2021Ernie Chen et al.stained with PE anti-human MRGX2 Antibody (359003; BioLegend) or PE Mouse IgG2b k Isotype Control (400314; BioLegend) at a concentration of 1.25 ul of antibody per 1 million cells in a final volume of 100 ul

(14) Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data FilesMethods and ProtocolsMarch 30, 2021Rebecca Grant, Karen Coopman, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzingantibody (Cat Number: 362552, Lot Number: B210221) PE Mouse IgG2b k Isotype Control antibody (Cat Number: 400313, Lot Number: B246304) BioLegend BV421 anti-human CD56 antibody (Cat Number: 423101, Lot Number: B246952) Brilliant

(15) YTHDF1 and YTHDF2 are associated with better patient survival and an inflamed tumor-immune microenvironment in non-small-cell lung cancerOncoimmunologyJanuary 1, 2021Tsuchiya K, Yoshimura K, Inoue Y, Iwashita Y, Yamada H, Kawase A, Watanabe T, Tanahashi M, Ogawa H, Funai K, et al.anti-human CD274 (B7-H1, PD-L1) antibody (cat #329706, Biolegend) or the corresponding isotype control (cat #400314, Biolegend) at 4°C for 30 minutes. A Gallios flow cytometer and the FlowJo software were used for data

(16) Karyopherin-β1 Regulates Radioresistance and Radiation-Increased Programmed Death-Ligand 1 Expression in Human Head and Neck Squamous Cell Carcinoma Cell LinesCancers (Basel)April 8, 2020Masaharu Hazawa, Hironori Yoshino, Yuta Nakagawa, Reina Shimizume, Keisuke Nitta, Yoshiaki Sato, Mariko Sato, Richard W. Wong, Ikuo Kashiwakura(PE)-labeled anti-human PD-L1 monoclonal antibody (#329706), PE-conjugated anti-mouse IgG2b antibody (#400314), and anti-p63(ΔN) antibody (#619001) were purchased from BioLegend (San Diego, CA, USA). Ambion Silencer® Select Pre-designed siRNA

(17) Anti-inflammatory effects of Lactobacillus johnsonii L531 in a pig model of Salmonella Infantis infection involves modulation of CCR6⁺ T cell responses and ER stressVeterinary researchFebruary 24, 2020Yang GY, Xia B, Su JH, He T, Liu X, Guo L, Zhang S, Zhu YH, Wang JF.

(18) C-type lectin receptors Mcl and Mincle control development of multiple sclerosis-like neuroinflammationThe Journal of clinical investigationFebruary 3, 2020N'diaye M, Brauner S, Flytzani S, Kular L, Warnecke A, Adzemovic MZ, Piket E, Min JH, Edwards W, Mela F, et al.experiment. Cells were stained for MCL (clone 9B9, catalog 360204) and isotype (clone MPC-11, catalog 400314) expression prior to in vitro stimulations (BioLegend). Upon stimulation, cells were stained with LIVE/DEAD

(19) Effect of pregnancy hormone mixtures on cytokine production and surface marker expression in naïve and LPS-activated THP-1 differentiated monocytes/macrophagesInnate immunityFebruary 1, 2020Mendoza-Cabrera MI, Navarro-Hernández RE, Santerre A, Ortiz-Lazareno PC, Pereira-Suárez AL, Estrada-Chávez C.

(20) NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute PancreatitisGastroenterologyJanuary 1, 2020Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss FU, Homuth G, De Freitas Chama LL, Mishra N, Mahajan UM, et al.

Write a Review

(1)

1/30/2019Staining Cultured MSC with Mouse IgG2b PE Isotype Control Adam GuessThis antibody was used as an isotype control to account for non-specific background staining in cultured MSC. It was chosen because it was the recommended isotype control for an antibody in a ... Read Review
(1)

2/9/2016Excellent PE Isotype Mouse IgG2b Linda HammerichThis antibody was used as an isotype control in intracellular flow cytometry and compared to anti-HMGB1-PE to determine the specifity of the HMGB-1 antibody.

PE Mouse IgG2b, kappa isotype Ctrl from BioLegend

Description

BioLegend

Citations (39)

Reviews

PE Mouse IgG2b, kappa isotype Ctrl from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map