Fig 1: Atrazine-induced expression of ATR, ATRIP and phosphorylation of Chk1 (Ser317) in MCF-10A cells. (A) Dose-response of atrazine–induced ATR, ATRIP and p-Chk1. MCF-10A cells (3 × 105) were incubated with the indicated concentrations of atrazine for 6 h and analyzed by Western blot; (B) time course of atrazine-induced ATR, ATRIP and p-Chk1in MCF-10A cells. MCF-10A cells (3 × 105) were treated with 0.1 µg/mL of atrazine for the indicated times and analyzed by Western blot. For each blot, the resulting fold-change of atrazine-mediated ATR, ATRIP and p-Chk1 expression over DMSO untreated controls is provided in the corresponding histogram (n = 3) with the signal normalized to actin as a loading control. * p < 0.05, ** p < 0.01 vs. controls.