Western Blot analysis of Protein Kinase C alpha 25 μg of A549, Hepg2 and K562 were loaded in to lanes 2, 3 and 4 respectively. Cell lysates were run under reducing conditions and transferred onto a nitrocellulose membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. Mouse anti Protein Kinase C alpha was used as the primary antibody at a dilution of 1/1000 in lanes 1-4. Goat anti Mouse IgG conjugated to HRP (STAR207P) was used as the secondary reagent at 1/10000. Membranes were visualized on the ChemiDoc MP Imaging System.