RAT ANTI MOUSE CD68 MCA1957 from Bio-Rad (Formerly AbD Serotec)

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Journal of neuroinflammation
July 12 2022

Fig 1: CCL2 is not required for macrophage recruitment either to the DRG or distal sciatic nerve 7 DPI. A–D Representative images showing macrophages marked by CD68 immunostaining in L4 DRGs ipsilateral (B, D) and contralateral (A, C) to a sciatic nerve transection. Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (B vs. D). E Macrophages were quantified by CD68 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 6, Ccl2 KO n = 8. F–I Representative images showing macrophages marked by Iba1 immunostaining in L4 DRGs ipsilateral (G, I) and contralateral (F, H) to a sciatic nerve transection. Again, Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (G vs. I). J Macrophages were quantified by Iba1 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 7, Ccl2 KO n = 8. K–N Representative images showing macrophages marked by CD68 immunostaining in distal sciatic nerves ipsilateral (L, N) and contralateral (K, M) to a sciatic nerve transection. Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (L vs. N). O Macrophages were quantified by CD68 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 9, Ccl2 KO n = 8. P–S Representative images showing macrophages marked by Iba1 immunostaining in distal sciatic nerves ipsilateral (Q, S) and contralateral (P, R) to a sciatic nerve transection. Again, Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (Q vs. S). T Macrophages were quantified by Iba1 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 8, Ccl2 KO n = 8. # Indicates a significant (p < 0.05) difference between Sh and Ax within a genotype. Scale bar is 50 µm
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eLife
June 21 2021

Fig 2: Ketogenic diet protects old mice from A59 (mCoV) infection by alleviation of inflammation.(A–E) Gene expression analysis of inflammatory cytokines (A–C) and components of inflammasome (D, E) in lung, VAT, and hypothalamus of A59 (mCoV)-infected old mice fed chow (Old-Chow, 20–21 months, n = 6) or KD (Old-KD, 20–21 months, n = 5). (F, G) Western blot analysis of caspase-1 inflammasome activation (F) in VAT of infected Old-Chow and Old-KD mice with quantification (G). (H) Representative immunofluorescence images of CD68 expression, phalloidin, and DAPI in hearts isolated from Old-Chow and Old-KD mice (left) and CD68+ cells/heart area analysis in heart of infected Old-Chow and Old-KD mice (right). (I) Flow cytometry analysis of ?d T cell in lung of infected Old-Chow and Old-KD mice. Error bars represent the mean ± S.E.M. Two-tailed unpaired t-tests were performed for statistical analysis. *p<0.05; **p<0.01.
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Frontiers in Cell and Developmental Biology
April 08 2021

Fig 3: SCFA supplementation resulted in less chronic inflammation at day 28 after FAN induced AKI. SCFA treated mice developed less severe chronic kidney injury, as evidenced by lower serum creatinine (A), BUN (B), histological injury scores (C) on PAS staining, and diminished collagen deposition (D) on PSR staining at day 28. Reduced CD4+, CD8+ T cell (E,F), CD68+ macrophage (G) and CD11+ dendritic cell (H) infiltration was seen in SCFA treated FAN kidneys, quantified by immunostaining. (I) SCFAs diminished mRNA expression of innate immune receptors (TLR2 and TLR4), pro-inflammatory cytokines (IL6, TNFa, and IFN?), chemokines (CCL2, CXCL2, and CXCL10), inflammasome components (NLRP3, ASC, IL18, and IL1ß), inflammatory mediator (iNOS), proximal tubule injury (KIM1), and fibrosis related genes (TGFß1, MMP2, and MMP9) measured by real-time PCR in kidney tissue at day 28. FAN (n = 12); non-FAN (n = 6). Data are shown as means ± SD or mean ± SEM; *P < 0.05, **P < 0.01, *** &P < 0.001, **** #P < 0.0001.
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Scientific reports
July 30 2019

Fig 4: Macrophage depletion is associated with reduced AVF wall thickness and patency. (A) Representative photomicrographs showing AVF wall thickness and number of a-actin+ cells in mice treated with clodronate vs. control (day 21). Scale bar, 25 µm. L, lumen (B) Bar graphs showing AVF wall thickness (left) and number of a-actin+ cells (right) in AVF after control or clodronate treatment; *p = 0.0005 (t test); n = 5. a-actin+ cell number: *p < 0.0001 (t test); n = 5. (C) Representative photomicrographs showing dual IF for a-actin (green) and p-Akt1 (red, first row), p-mTORC1 (red, second row) or p-mTORC2 (red, third row) in AVF after control or clodronate treatment (day 21). (D) Bar graph showing quantification of dual IF in AVF after control or clodronate treatment. p-Akt1-a-actin: *p < 0.0001 (t test); n = 5. p-mTORC1-a-actin: *p = 0.0011 (t test); n = 5. p-mTORC2-a-actin: p = 0.5549 (t test); n = 5. (E) Photomicrographs showing representative IF of cleaved caspase-3 (red) merged with ICAM, a-actin or CD68 (green), and DAPI (blue) in AVF of control or clodronate treated mice (day 7); L, lumen; scale bar, 25 µm. White arrowheads indicate merged signal. (F) Bar graphs showing percentage of dual positive cells (day 21). Cleaved caspase-3-ICAM: p > 0.9999 (t test); n = 4–5. Cleaved caspase-3-a-actin: p = 0.9315 (t test). n = 4–5. Cleaved caspase-3-CD68: *p = 0.0027 (t test). n = 4–5. (G) Photomicrographs showing representative IF of PCNA (red) merged with ICAM, a-actin or CD68 (green), and DAPI (blue) in AVF of control or clodronate treated mice (day 7); L, lumen; scale bar, 25 µm. White arrowheads indicate merged signal. (H) Bar graph showing percentage of dual positive cells (day 21). PCNA-ICAM positive cells: p = 0.8139 (t test); PCNA-a-actin: *P = 0.0035 (t test); PCNA-CD68: p = 0.8547 (t test). n = 4–5. (I) Representative photomicrographs showing dual IF for CD68 (red) and iNOS (green, top row), TNF-a (green, second row), IL-10 (green, third row) or CD206 (green, bottom row) in AVF after control or clodronate treatment; day 21. Scale bar, 25 µm. L, lumen. (J) Bar graphs showing quantification of dual IF after control or clodronate treatment (day 21). CD68-iNOS: p = 0.7311 (t test). CD68-TNF-a: p < 0.8422 (t test). CD68-IL-10: p < 0.0001 (t test). CD68-CD206: p = 0.0006 (t test). n = 5. (K) Line graph showing AVF patency rate in mice treated with control or clodronate IP injections. *P = 0. 0.0372 (Log-rank), n = 6–7 in each arm.
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Scientific reports
July 30 2019

Fig 5: Reduced Akt1 and mTORC1 but not mTORC2 phosphorylation with rapamycin. (A) Photomicrographs showing representative IF of p-Akt1+ (top, red), p-mTORC1+ (middle, red) and p-mTORC2+ (bottom, red) cells in control or rapamycin treated mice AVF (day 7). Scale bar, 25µm. L, lumen. (B) Bar graphs showing number of p-Akt1+, p-mTORC1+ and p-mTORC2+ cells in AVF after rapamycin or control treatment. p-Akt-1: *p < 0.0001 (ANOVA); *p < 0.0001, day 7; *p = 0.0105, day 21 (post hoc); n = 4–5. p-mTORC1: p < 0.0001 (ANOVA); *p < 0.0001, day 3; *p < 0.0001, day 7 (post hoc); n = 4–5. p-mTORC2: p = 0.2870 (ANOVA); n = 4–5. (C) Representative Western blot showing Akt1, mTORC1, mTORC2 phosphorylation level after control vs rapamycin treatment. (D) Graphs showing densitometry measurement of Akt1, mTORC1 and mTORC2 phosphorylation. p-Akt1: tAkt1, p = 0.0002 (ANOVA); *p = 0.0110, day 7; *p = 0.0359, day 21 (post hoc); n = 3. p-mTORC1: tmTORC1, p = 0.0004 (ANOVA); *p = 0.0157, day 3; *p = 0.0192, day 7; *p = 0.0366, day 21 (post hoc); n = 3. p-mTORC2: tmTORC2: P = 0.9893 (ANOVA); n = 3. (E) Photomicrographs showing representative IF of dually-positive a-actin (green) and p-Akt1 (red, first row) or p-mTORC1 (red, second row) in AVF after control or rapamycin treatment (day 7). (F) Bar graphs showing quantification of dual IF after control vs rapamycin treatment. P-Akt1-a-actin: p < 0.0001 (ANOVA); *p = 0.0002, day 7; *p = 0.0017, day 21 (post hoc); n = 4–5. p-mTORC1-a-actin: p < 0.0001 (ANOVA); *p = 0.0136, day 7; *p < 0.0001, day 21 (post hoc); n = 4. (G) Photomicrographs showing representative dual IF for CD68 (green) and p-Akt1 (red, top row) or p-mTORC1 (red, bottom row) in AVF after control or rapamycin treatment (day 7). (H) Bar graphs showing quantification of dual IF after control vs rapamycin treatment. p-Akt1-CD68: p < 0.0001 (ANOVA); *p = 0.0013, day 7; *p = 0.0183, day 21 (post hoc); n = 4–5. p-mTORC1-CD68: p < 0.0001 (ANOVA); *p < 0.0001, day 3; *p < 0.0001, day 7 (post hoc); n = 4–5. Data represent mean ± SEM.

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RAT ANTI MOUSE CD68

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Bio-Rad (Formerly AbD Serotec)'s RAT ANTI MOUSE CD68 is a Rat monoclonal antibody. The RAT ANTI MOUSE CD68 Antibody was generated using CD68 as the antigen. It reacts with Mouse. This antibody has been shown to work in applications such as: Histology, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - frozen, Immunoprecipitation, and Western Blot.

Product Specs
ItemRAT ANTI MOUSE CD68
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMCA1957
NCBI Gene Aliasesgp110, Lamp4, Scard1
TargetCD68
CloneFA-11
FormatPurified IgG - liquid
ApplicationsWestern Blotting,Immunohistology - Paraffin,Immunoprecipitation,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence
Quantity0.25 mg
IsotypeIgG2a
HostRat
ReactivityMouse
ImmunogenPurified Concanavalin A acceptor glycoprotein from P815 cell line
ClonalityMonoclonal Antibody
NCBI Full Gene NameCD68 antigen
ConcentrationIgG concentration 1.0 mg/ml

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12/27/2021Rat Anti-CD68 Antibody for Immunofluorescence José VieiraVery good antibody that works perfectly using a high dilution.
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1/7/2016Mouse Skin CD68 Lin ChenOur goal is to examine macrophages in mouse skin using IF. We tried other macrophage antibodies such Moma 2 and F4/80, this clone (FA-11) worked much better than others
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7/1/2015Excellent recognition of CD68+ macrophages in various tissues of the mice Rahul KeswaniMacrophages can be identified in tissue cross-sections via the CD68 intracellular marker. We used this product along with the use of Cell Signaling Tech's AlexaFluor488 (Catalog No. 4416S) secondary ... Read Review

Journal of neuroinflammation

eLife

Frontiers in Cell and Developmental Biology

Scientific reports

Scientific reports

RAT ANTI MOUSE CD68 from Bio-Rad (Formerly AbD Serotec)

Bio-Rad

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RAT ANTI MOUSE CD68 from Bio-Rad (Formerly AbD Serotec)

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