Fig 1: Validation of de novo proteomic comparison of trained vs non-trained.RC3xCamk2a-Cre mice using BONCAT western blotting BONCAT-WB confirms that in trained mice, there is increased hippocampal synthesis of a- a-adaptin (Ap2a1), neuron specific enolase (NSE: Eno2), V-ATPase subunit B2 (V-ATPase B2: Atp6v1b2), and the a isoform of the structural subunit of protein phosphatase 2A (PP2A-A: Ppp2r1a) compared to non-trained controls. Synthesis of the ARE binding protein ARE/poly(U)-binding/degradation factor 1 (AUF-1: Hnrnpd) was also confirmed to be decreased during spatial LTM formation. The synthesis of the housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was unchanged in the hippocampal neurons of trained RC3xCamk2a-Cre mice compared to non-trained controls (p=0.48), together validating the SWATH analysis (n = 4 mice, Student’s paired t-test, *p=0.05, **p=0.01, ***p=0.001).