Fig 1: AAV-miR-reduced CACNB1, RGS4, and GNB1. (A) Luciferase plasmid activity in HEK293 cells with TTS-miRs for ADRBK1, CACNA1C, CACNB1, CACNB2, GNB1, GNG12, RGS3, and RGS4. n/N = 9/3, ‘n’ = transfections and ‘N’ = biological repeats, significance with ‘N’ by Student’s unpaired t-test. RTqPCR of CACNA1C (B, N = 8), CACNB1 (C, N = 8), RGS4 (D, N = 8), GNB1 (E, N = 8), and GNG12 (F, N = 8) from apex/base of AAV-treated rat hearts. Western blot for CACNB1 (G), RGS4 (H), and GNB1 (I) from apex/base of AAV-treated rat myocardium with representative blots and molecular weights shown (kDa, J). For G–I, N = 7 for AAV-control and AAV-miR in apex and AAV-miR in base, and N = 6 for AAV-control in base. Mean ± SEM displayed throughout. For B–I ‘N’=number of rats. Significance by two-way ANOVA, with Bonferroni’s post hoc: *P < 0.05, **P < 0.01, ***P < 0.001.