Fig 1: Lack of mammary oncogenesis is marked by NKT expansion and CD1d+ MECs(A) Flow cytometry analysis of mammary resident NKTs (CD45+NK1.1+CD3+) from DOX-treated, nulliparous (left panel, n = 5) and parous (right panel, n = 5) CAGMYC female mice. *p = 0.002.(B) Flow cytometry quantification of CD1d+ luminal and myoepithelial MECs from DOX-treated, nulliparous (left panel, n = 16) and parous (right panel, n = 11) CAGMYC female mice. *p = 0.02.(C) Mammary tumor-free survival plot of nulliparous (black line, n = 5) and parous (pink line, n = 5) Brca1KO female mice.(D) H&E-stained histological images from mammary tissue and tumors from nulliparous (top panels) and parous (bottom panels) Brca1KO female mice. Scale: 5 mm. Zoom-in panels, scale: 500 µm.(E) Flow cytometry quantification of CD1d+CD24high luminal MECs from Brca1KO pre-pregnancy mammary tumors (black bar, n = 3), Brca1KO post-pregnancy healthy mammary tissue (pink bar, n = 4), and Brca1KO post-pregnancy mammary tumor (blue bar, n = 1). *p = 0.02.(F) Flow cytometry analysis of NKTs in normal mammary tissue from nulliparous, tumor-bearing, Brca1KO female mice (left panel, n = 4) and normal mammary tissue from healthy parous Brca1KO female mice (right panel, n = 4). *p = 0.003.(G) Quantification of ?dNKTs in normal mammary tissue from nulliparous, tumor-bearing, Brca1KO female mice (black bar panel, n = 4), in mammary tumor tissue from nulliparous Brca1KO female mice (blue bar, n = 3), and in normal mammary tissue from healthy parous Brca1KO female mice (black bar panel, n = 2). *p = 0.023 and **p = 0.008.For all analyses, error bars indicate standard error of mean across samples of the same experimental group. Statistically significant differences were considered with Student’s t test p < 0.05. See also Figures S8-S12.