Fig 1: Exogenous Galectin-8 Favors T-B Cooperation In Vivo(A) Schematic of the experimental approach used to assess the ability of B cells to present antigen to CD4+ T cells in vivo. The western blot shows the amounts of antigen (HEL) effectively immobilized at the surface of 0.2-µm microspheres in the different conditions used to immunize mice. H, HEL alone; H-B, HEL-BSA; H-G, HEL-Galectin-8; H-B-O, HEL-BSA-OVA; H-G-O, HEL-Galectin-8-OVA. Numbers below the blot represent the normalized density of the bands with respect to the HEL alone condition. Data are representative of N = 3 independent experiments.(B and C) Representative dot plots used for the gating of (B) HEL-specific GC B cells (CD45.2+B220+HEL+GL7+FAS+) and (C) OVA-specific OT-II Tfh cells (CD45.2+CD4+CXCR5+PD1+).(D and E) Quantification of the number of (D) HEL-specific GC B cells and (E) OVA-specific OT-II Tfh cells following mouse immunization with indicated microspheres. n = 10 mice per condition pooled from N = 3 independent experiments.(F and G) Quantification of the number of (F) HEL-specific GC B cells and (G) OVA-specific OT-II Tfh cells in CD45.1 Lgals8+/+ or Lgals8-/- recipient mice (adoptively transferred with CD45.2 Lgals8+/+ SWHEL B cells and OT-II CD4+ T cells) following their immunization with indicated microspheres. n = 6 mice per condition pooled from N = 2 independent experiments. Kruskal-Wallis test was used to assess statistical significance. All bar graphs indicate mean ± SEM.