Fig 2: Periodontal inflammation can promote head and neck metastasis. a Timeline of the late metastasis model, PI was induced with LPS for 3 weeks then mice were injected in the mammary fat pad with 4T1 cells, the primary tumor was let to grow until it reached 100 mm3 in size, surgery was accomplished to extract this tumor and animals were let to grow metastasis and euthanized at 10.5 weeks. b Metastasis were observed and counted in lungs and lymph node in the individual animal groups in the presence or absence of anakinra. (n = 3; ANOVA). c IHC staining of Pan-cytokeratin and Gr1 from the lymph node and the cervical tissue of late metastasis model. (n = 3; Ank anakinra, ANOVA, *P < 0.05; **P < 0.01; ****P < 0.0001)