IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 (Product # 16-0039-81) and anti-Human CD28 (Product # 16-0289-81) in the presence of Human IL-23 Recombinant for 3 days, followed by 3 days with IL-23 alone. Day 6 cultures were treated with Brefeldin A alone (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were run under reducing conditions, immunoblotted with 2 µg/mL of Anti-Human IL-17F Purified and revealed with Anti-Mouse HRP.