Mouse splenocytes were stimulated with Con A for 2 days, followed by Mouse IL-2 Recombinant Protein (Product # 14-8021-64) and Mouse IL-4 Recombinant Protein (Product # 14-8041-80) for 3 days, and re-stimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0032-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A (Product # 00-4506-51) for 5 hours. The cells were surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82) and intracellularly stained with Anti-Mouse IFN gamma FITC.
Supplier Page from Thermo Fisher Scientific for IFN gamma Antibody FITC