Fig 1: Quercetin enhances STL1 sensitivity in HG-3 through AKT inhibition. Immunoblot of phospho-AKT and AKT protein expressions was performed on HG-3 cells following 60 min of treatment with 10 µM quercetin (QUE), 20 µM STL1, and their association with respect to the DMSO-treated (0.2%) cells. After cell lysis and immunoblotting, membranes were incubated for 16 h at 4 °C with anti-phospho-AKT (pAKTSer473) and re-probed with anti-AKT1-2-3 polyclonal antibodies. Finally, the membranes were probed again with an anti-a-tubulin monoclonal antibody. Band intensities were quantified by measuring the optical density and analyzed by the Multi-Analyst Software. The result of the densitometric analysis is reported in the immunoblot and is indicated by the numbers between AKT1-2-3 and a-Tubulin panels. Values indicated the AKT1-2-3 expression normalized vs. a-tubulin. The image is representative of one out of two independent immunoblots performed.