Fig 2: CAC1 knockdown increased sensitivity of SW480/5-FU cells to 5-FU by influencing P-glycoprotein and MRP-1 expression.(A) SW480 and SW480/5-FU cells were transfected with shCAC1 and shCON for one week, and subjected to FACS analysis with Annexin V-FITC and propidium iodide staining. (B) The expression of drug resistance-associated proteins, P-glycoprotein and MRP-1, in SW480 and SW480/5-FU cells after transfection with shCAC1 and shCON was examined by western blot. *P<0.05, **P<0.01.

Fig 3: CAC1 knockdown increased sensitivity of LoVo/5-FU cells to 5-FU by influencing P-glycoprotein and MRP-1 expression.(A) LoVo and LoVo/5-FU cells transfected with shCAC1 and shCON were subjected to FACS analysis with Annexin V-FITC and propidium iodide staining. (B) The expression of drug resistance-associated proteins, P-glycoprotein and MRP-1, in LoVo and LoVo/5-FU cells after transfection with shCAC1 and shCON was examined by western blot. *P<0.05, **P<0.01.

Fig 4: CAC1 knockdown reduced colony formation and invasive and migration ability of SW480 and SW480/5-FU cells.(A) Colony formation assay, (B) cell migration assay and (C) cell invasion assay were detected in SW480 and SW480/5-FU cells were stably transfected with shCAC1 and shCON for one week. *P<0.05, **P<0.01.

Fig 5: The overall survival curve of colorectal cancer patients with different CAC1 expression.(A) The overall survival curve of the 83 enrolled patients. (B) The overall survival curves of patients with CAC1 positive expression and CAC1 negative expression.