Fig 2: ILC2 and EOS interactions with ECs, DCs, and monocytes. A) Immunoblot detected ICAM-1 and VCAM-1 expression from aortic ECs pretreated with or without EOS lysates from WT mice or ILC2 lysates from WT or Il5-/- mice. B/C) Mouse aortic ring assay representative images and quantification. bFGF was used as positive controls. Scale: 400 µm. D) FACS analysis of bone-marrow-derived DCs cultured with or without EOS lysates and ILC2 lysates from WT mice. FACS analysis of CD11b+Ly6Chi monocytes after bone-marrow-derived monocytes were treated with E) LPS and INF-?, and F) CD11b+Ly6Clo monocytes after cells were treated with IL4, with and without EOS lysates and ILC2 lysates from WT mice. Representative images in panels (A) and (D) are shown to the left. Data are mean±SEM. n = 3–7 per group. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA test.