5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9] A-1018 from EpiGentek

Product Specs
Item5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA-1018
Quantity10 µg (Trial Size), 50 µg, 100 µg
ApplicationsIF, IHC, ELISA, DB, hMeDIP
Conjugate/TagUnconjugated
PurityAffinity Purified
ReactivityBroad Range
Target5-hmC
IsotypeIgG1
CloneHMC/4D9
HostMouse
ClonalityMonoclonal
Research AreaDNA Methylation
Concentration1 mg/ml
Format10 mM phosphate buffer, 150 mM NaCl, pH 7.4
Antibody TypePrimary Antibody

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

Email: info@epigentek.com
sales@epigentek.com
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support@epigentek.com

Company Profile

Website: www.epigentek.com

(1) Oscillatory shear stress-induced downregulation of TET1s injures vascular endothelial planar cell polarity by suppression of actin polymerizationAPL BioengineeringSeptember 1, 2023Kai Qu, Caihong Wang, Lu Huang, Xian Qin, Kun Zhang, Juhui Qiu, Guixue Wangmilk for 1 h at room temperature. Then, the membrane was incubated in a monoclonal anti-5hmC antibody (A-1018-100, Epigentek) or anti-5mC antibody (A-1014-100, Epigentek) at 4 °C overnight. The corresponding secondary

(2) The intrinsic and extrinsic effects of TET proteins during gastrulationCellAugust 18, 2022Cheng S, Mittnenzweig M, Mayshar Y, Lifshitz A, Dunjić M, Rais Y, Ben-Yair R, Gehrs S, Chomsky E, Mukamel Z, et al.secondary antibody incubation. Antibodies used for this assay: anti-5hmC (ActiveMotif, 39069; Epigentek, A-1018), Goat anti-Rabbit IgG (H+L), HRP (Invitrogen, 31460), Goat anti-Mouse IgG (H+L), HRP (Invitrogen, 31430).

(3) YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesisNature geneticsAugust 1, 2022Wu BK, Mei SC, Chen EH, Zheng Y, Pan D.detected using specific antibodies (anti-5mC, 1:500, Epigentek, A-1014; anti-5hmC, 1:2,000, Epigentek, A-1018) and visualized by SuperSignal West Dura Extended Duration Substrate (Thermo Scientific). Signal detection

(4) Reusable Single Cell for Iterative Epigenomic AnalysesJournal of Visualized ExperimentsFebruary 28, 2022Hidetaka Ohnuki, David J. Venzon, Alexei Lobanov, Giovanna TosatoCompany,200 proof,Ethanol 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9],Epigentek,A-1018-100,Anti-5hmC Acridine Orange/Propidium Iodide Stain,Logos Biosystems,F23001,Cell counter "Acrylamide

(5) Regular Intake of Green Tea Polyphenols Suppresses the Development of Nonmelanoma Skin Cancer through miR-29-Mediated Epigenetic ModificationsJournal of Clinical MedicineJanuary 13, 2022Vikash Kansal, Anshu Agarwal, Angela Harbour, Humaira Farooqi, Vijay Kumar Singh, Ram Prasadfrom cell signaling (Danvers, MA, USA). Antibodies specific for TET1 (#A-1020), TET3 (#A70559), 5hmC (#A-1018), ELISA assay kits for global DNA methylation (#P-1030), DNMT activity (#P-3009), and TET activity (#P-3086-96)

(6) TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosisInternational journal of biological sciencesJanuary 1, 2022Qu K, Wang C, Huang L, Qin X, Zhang K, Zhong Y, Ma Q, Yan W, Li T, Peng Q, et al.nonfat milk for 1 h at room temperature. Then, the membrane was incubated in a monoclonal anti-5hmC (A-1018-100, Epigentek) antibody and anti-5mC antibody (A-1014-100, Epigentek) at 4°C overnight. The corresponding

(7) PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islandsLife science allianceJanuary 1, 2022Wang X, Rosikiewicz W, Sedkov Y, Martinez T, Hansen BS, Schreiner P, Christensen J, Xu B, Pruett-Miller SM, Helin K, et al.Cell Signaling Technology) at 1:2,000. For dot blot analysis Mouse α-5mC (A-1014-050; Epigentek) at 1:2,000; mouse α-5hmC (A-1018-050; Epigentek) at 1:2,000; rabbit α-5fC (74178S; Cell Signaling Technology) at 1:2,000; rabbit α-5caC

(8) MeCP2 regulates Tet1-catalyzed demethylation, CTCF binding, and learning-dependent alternative splicing of the BDNF gene in TurtleeLifeJune 8, 2017Zhaoqing Zheng, Ganesh Ambigapathy, Joyce Keiferthen the supernatant was immunoprecipated with antibody against 5-hydroxymethylcytosine (Epigentek, A-1018) or normal IgG for 2 hr at 4°C on a rotating platform. Immune complexes were collected by incubating

(9) Influence of smoking on methylation and hydroxymethylation levels in global DNA and specific sites of KRT14, KRT19, MIR-9-3 and MIR-137 genes of oral mucosaArchives of oral biologyDecember 1, 2016Ludimila de Araújo Costa, Isabelle Cristina Borba da Silva, Bruno Augusto Linhares Almeida Mariz, Mikaelly Batista da Silva, Gláucia Marques Freitas-Ribeiro, Naila Francis Paulo de Oliveira

(10) TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicingThe EMBO journalFebruary 1, 2016Marina RJ, Sturgill D, Bailly MA, Thenoz M, Varma G, Prigge MF, Nanan KK, Shukla S, Haque N, Oberdoerffer S.anti-5-methylcytidine [A-1014 (Epigentek); 006-500 (Diagenode) for MedIP-seq], anti-5-hydroxymethylcytidine [A-1018 (Epigentek); 39769 (Active Motif) for MedIP-seq], anti-5-carboxylcytosine [RM24 1-A3] (Abcam), normal rabbit IgG

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5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]

5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9] from EpiGentek

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5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9] from EpiGentek

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