PE anti-human CD4 300508 from BioLegend

Product Specs
ItemPE anti-human CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number300508
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
ApplicationsFlow Cytometry (FC)
ReactivityHuman
HostMouse
Conjugate/TagPE
Quantity100 tests
IsotypeIgG1, Kappa
CloneRPA-T4
Antibody TypePrimary
NCBI Full Gene NameCD4 molecule
Formatµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Y RNAs are conserved endogenous RIG-I ligands across RNA virus infection and are targeted by HIV-1iScienceJuly 15, 2022Vabret N, Najburg V, Solovyov A, Gopal R, McClain C, Šulc P, Balan S, Rahou Y, Beauclair G, Chazal M, et al.anti-CD3-Pacific Blue (mouse monoclonal, Biolegend #300330); anti-CD4-PE (mouse monoclonal, Biolegend, #300539); anti-CD19-APC (mouse monoclonal, Biolegend #302212); anti-CD14-APC (mouse monoclonal, Biolegend, #325608);

(2) An anti-PD-1–GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapyNature CancerMarch 7, 2022Sarah Chan et al.

(3) In Vitro Characterization of Sphingosine 1-Phosphate Receptor 1 (S1P1) Expression and Mediated Migration of Primary Human T and B Cells in the Context of Cenerimod, a Novel, Selective S1P1 Receptor ModulatorInternational Journal of Molecular SciencesJanuary 21, 2022Lisa Schlicher, Paulina Kulig, Audrey von Münchow, Mark J. Murphy, Marcel P. Keller130-113-525), S1P1 receptoreFluor 660 (eBioscience, 50-3639-42), CD19PE (BioLegend, 302208), CD4PE (BioLegend, 300508). Every condition was performed with technical replicates. One representative experiment from one healthy

(4) Impaired local intrinsic immunity to SARS-CoV-2 infection in severe COVID-19CellSeptember 2, 2021Carly G.K. Ziegler, Vincent N. Miao, Anna H. Owings, Andrew W. Navia, Ying Tang, Joshua D. Bromley, Peter Lotfy, Meredith Sloan, Hannah Laird, Haley B. Williams, et al.SK1),BioLegend,Cat# 344714; RRID: AB_2044006 PE-conjugated anti-human CD4 (clone: RPA-T4),BioLegend,Cat# 300508; RRID: AB_314076 Brilliant Violet 786-conjugated anti-human CD326 (clone: 9C4),BioLegend,Cat# 324237;

(5) Differential impacts of TNFα inhibitors on the transcriptome of Th cellsArthritis research & therapyJuly 23, 2021Ho CH, Silva AA, Tomita B, Weng HY, Ho IC.performed according to manufacturer’s instructions. FACS PBMCs were stained with anti-CD4 (#317450 and #300508, Biolegend), anti-CD8 (#344750 and #344714, Biolegend), anti-CD14 (#301815, Biolegend), anti-CD19 (#332224,

(6) Longitudinal analysis reveals that delayed bystander CD8+ T cell activation and early immune pathology distinguish severe COVID-19 from mild diseaseImmunityJune 8, 2021Bergamaschi L, Mescia F, Turner L, Hanson AL, Kotagiri P, Dunmore BJ, Ruffieux H, De Sa A, Huhn O, Morgan MD, et al.

(7) Interrogating the small intestine tuft cell – ILC2 circuit using in vivo manipulationsCurrent ProtocolsMarch 26, 2021Claire E. O'Leary, Xiaogang Feng, Victor S. Cortez, Richard M. Locksley, Christoph SchneiderLife Technologies A-21206; PE: Biolegend 406421) Anti-Thy1.2 BV785 (Biolegend 105331) Anti-human CD4 (PE Biolegend 300508; APC Biolegend 300514) Anti-IL-17RB APC (Biolegend 146308) Anti-KLRG1 eFluor710 (ThermoFisher 46-5893-82) Anti-Gata3

(8) Expansion of Functional Myeloid-Derived Suppressor Cells in Controlled Human Malaria InfectionFrontiers in ImmunologyMarch 19, 2021Carlos Lamsfus Calle, Rolf Fendel, Anurag Singh, Thomas L. Richie, Stephen L. Hoffman, Peter G. Kremsner, Benjamin Mordmüllerdays of incubation, cells were harvested and stained with mouse IgG1, κ anti-human CD4-PE (Biolegend, #300508, Clone RPA-T4) and mouse IgG1, κ anti-human CD8a-APC (Biolegend, #300912, Clone HIT8a). Shortly before measurement,

(9) TCR β chain-directed bispecific antibodies for the treatment of T cell cancersScience translational medicineMarch 10, 2021Paul S, Pearlman AH, Douglass J, Mog BJ, Hsiue EH, Hwang MS, DiNapoli SR, Konig MF, Brown PA, Wright KM, et al.APC-anti- human CD19 (clone HIB19, BioLegend, #302212), phycoerythrin (PE)–anti-human CD4 (clone RPA-T4, BioLegend, #300508), APC-anti-human CD8 (clone SK1, BioLegend, #344722), PE-anti-human Cβ1 TCR (clone JOVI.1 BD Biosciences,

(10) Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer CellsBiology (Basel)February 10, 2021Daria S. Chulpanova, Zarema E. Gilazieva, Sevindzh K. Kletukhina, Aleksandr M. Aimaletdinov, Ekaterina E. Garanina, Victoria James, Albert A. Rizvanov, Valeriya V. Solovyevaend of the incubation, PBMCs were collected and labeled with anti-CD4 and ani-CD8a antibodies (PE, #300508 and APC, #300912, respectively, BioLegend, San Diego, CA, USA), and CFDA fluorescence was analyzed using

(11) Human primed ILCPs support endothelial activation through NF-κB signalingeLifeFebruary 8, 2021Vanoni G, Ercolano G, Candiani S, Rutigliani M, Lanata M, Derré L, Marcenaro E, Schneider P, Romero P, Jandus C, et al.

(12) Oxidative Stress Induces Mitochondrial Compromise in CD4 T Cells From Chronically HCV-Infected IndividualsFrontiers in immunologyJanuary 1, 2021Schank M, Zhao J, Wang L, Nguyen LNT, Cao D, Dang X, Khanal S, Zhang J, Zhang Y, Wu XY, et al.T cell phenotype analysis, the following fluorescence-conjugated antibodies were used: CD4-PE (Cat# 300508) or CD4-FITC (Cat# 300506) with CD45RA-PerCP (Cat# 304156) and CD71-A647 (Cat# 334118, BioLegend; San

(13) Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIVFrontiers in immunologyJanuary 1, 2021Zhao J, Schank M, Wang L, Li Z, Nguyen LN, Dang X, Cao D, Khanal S, Nguyen LNT, Thakuri BKC, et al.T cell phenotype analysis, the following fluorescence-conjugated antibodies were used: CD4-PE (Cat #300508), CD45RA-PerCP (Cat# 304156), and CD71-A647 (Cat #334118) or CD4-PerCP (Cat #300527), CD71-A647 (Cat

(14) Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathwayCell death & diseaseDecember 2, 2020Schank M, Zhao J, Wang L, Li Z, Cao D, Nguyen LN, Dang X, Khanal S, Nguyen LNT, Thakuri BKC, et al.flow cytometry, the following fluorochrome-conjugated antibodies were used: PE anti-human CD4 (Cat #300508), PerCP anti-human CD45RA (Cat# 304156), Alexa Fluor 647 anti-human CD71 (Cat #334118) (all from BioLegend),

(15) Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligandsNature protocolsApril 1, 2020Chang ZL, Hou AJ, Chen YY.[clone L5] (BioLegend Cat# 637308, RRID:AB_2561497) PE anti-human CD4 [clone RPA-T4] (BioLegend Cat# 300508, RRID:AB_314076) APC anti-human CD8α [clone RPA-T8] (BioLegend Cat# 301049, RRID:AB_2562054) FITC anti-human CD3

(16) Tuft-Cell-Derived Leukotrienes Drive Rapid Anti-helminth Immunity in the Small Intestine but Are Dispensable for Anti-protist ImmunityImmunityMarch 17, 2020McGinty JW, Ting HA, Billipp TE, Nadjsombati MS, Khan DM, Barrett NA, Liang HE, Matsumoto I, von Moltke J.APC, clone G8.8",BioLegend,"Cat#118214, RRID: AB_1134102" "Human CD4 PE, clone RPA-T4",BioLegend,"Cat#300508, RRID: AB_314076" "CD45 BV605, clone 30-F11",BioLegend,"Cat#103155, RRID: AB_2650656" "CD45 BV650,

(17) Bioengineering the Oxygen-Deprived Tumor Microenvironment Within a Three-Dimensional Platform for Studying Tumor-Immune InteractionsFrontiers in bioengineering and biotechnologyJanuary 1, 2020Bhattacharya S, Calar K, Evans C, Petrasko M, de la Puente P.following antibodies: FITC conjugated anti-CD3 (300406, BioLegend, CA), PE-Cy5 conjugated anti-CD4 (300508, BioLegend, CA), APC-Cy7 conjugated anti-CD8 (344714, BioLegend, CA), APC conjugated anti-CD19 (302212,

(18) T lymphocytes migrate upstream after completing the leukocyte adhesion cascadeCell adhesion & migrationDecember 1, 2019Anderson NR, Buffone A Jr, Hammer DA.

(19) Single-Cell RNA Sequencing Resolves Spatiotemporal Development of Pre-thymic Lymphoid Progenitors and Thymus Organogenesis in Human EmbryosImmunityNovember 19, 2019Zeng Y, Liu C, Gong Y, Bai Z, Hou S, He J, Bian Z, Li Z, Ni Y, Yan J, et al.

(20) An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 developmentNature immunologySeptember 1, 2019Bagadia P, Huang X, Liu TT, Durai V, Grajales-Reyes GE, Nitschké M, Modrusan Z, Granja JM, Satpathy AT, Briseño CG, et al.

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(1)

12/29/2021Good CD4 Antibody Sushant KhanalCd4 T cell percentage was determined in total PBMC based on the following antibody staining.
(1)

10/30/2020Works Great! Dechao CaoWe use it to study CD4+ T cells in human PBMCs, trying to see CD4+ T cells apoptosis and cytokines.
(1)

3/3/2014CD3, CD4, CD8 Antibodies Alok JoglekarThese antibodies from BioLegend work extremely well. I used these to phenotype in vitro expanded primary T cells. These antibodies give me consistent results every time. Although the CoA says use 5 ul... Read Review

PE anti-human CD4 from BioLegend

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PE anti-human CD4 from BioLegend

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