Fig 2: Restoration of p53 in orthotopically transplanted lung adenocarcinomas causes an infiltration of CD45pos. immune cells and fibroblasts and elevated chemokine levels.a Histological sections from Control and p53-Restored tumors at Day 2, 6, and Day 10. H&E and IHC for GFP, CD45, and SMA. b Multiparameter cytokine detection in bronchioalveolar lavage (BAL) of Control and p53-Restored mice. Significantly changing cytokines as determined by t-test are indicated (IL-6, CCL2, CXCL1, M-CSF, and CCL4). c Multiparameter cytokine detection in KPrLG cell culture supernatant after p53 restoration in vitro

Fig 3: Plastic caging increases macrophages and neutrophils during acute myocarditis in BALB/c males. Male BALB/c mice were given normal drinking water (drinking water that did not contain BPA) for 2 weeks and housed either in glass cages/water bottles (glass) or plastic cages/water bottles (plastic) with soy-free food and bedding. BALB/c mice were housed in glass cages/water bottles with soy-free food and bedding and administered either normal drinking water (drinking water that did not contain BPA) (glass) or 250 µg BPA/L water (50 BPA) for 2 weeks. Mice were injected i.p. with 103 PFU of CVB3 on day 0 and harvested at day 10 pi. Five micron sections were stained with antibodies against (a) CD45 (total lymphocytes), (b) CD11b+ cells (i.e., macrophages, neutrophils, mast cells), (c) F4/80+ macrophages, (d) GR1 (neutrophils), (e) CD3+ T cells, and positive pixels were compared to total positive and negative pixels to determine % positive. Data shown as scatter plot and mean +/-SEM using one-way ANOVA with Holm–Šídák’s multiple comparisons test for 17–19 mice/group (* p < 0.05), (** p < 0.01), (**** p < 0.0001). Representative images of positive-stained cells (brown) in plastic caging (plastic), glass caging (glass) and 50 BPA in drinking water (50 BPA) in glass cages shown for (a) CD45, (b) CD11b, (c) F4/80, (d) GR1, and (e) CD3. Red arrow points to brown GR1-positive cells (magnification 200×, scale bar 200 µm).

Fig 4: Leucocytes closely associate with the mammary epithelium during involution, residing between the luminal and basal alveolar cells. Mammary glands from BALB/c mice were harvested at the indicated time points after forced involution, optically cleared and imaged using confocal microscopy. Images in (A–D) show 3D maximum intensity projections (MIPs) of the entire sequence captured and were immunostained for smooth muscle a-actin (SMA) in conjunction with (A) CD11c (yellow) and (B–D) MHCII (yellow); smaller images show individual stains of the merged image in the larger panels. (E, F) Individual optical slices (2.0 µm thick), from an optical stack (E = 72 h involution; F = 6 days of involution), where the depth (z value) is relative to the first image in each sequence. Glands were immunostained for SMA (magenta) and CD45 (cyan). Images are representative of four mice at each time point examined; all scale bars represent 100 µm; Dt, mammary duct; BV, blood vessel.

Fig 5: Characterization of damage in Achilles tendon after complete rupture. (a) Representative tissue sections of healthy and injured Achilles tendons stained for H&E; image scale bar 400 µm; insert scale bar 20 µm; (b) with inserts highlighting tissue microstructure in healthy and injured Achilles tendons and representative images of tendons immunostained for CD45/perilipin; image scale bar 400 µm; insert scale bar 20 µm; (c) Picrosirius red staining of tissue cryosections; image scale bar 400 µm; and (d–k) inserts pointing to areas visualizing collagen fiber arrangement under normal or polarized light and after collagen I immunostaining; image scale bar 20 µm. DAPI was used to identify all nuclei. dpi, days post-injury; PSR, Picrosirius red; Col I, collagen type I.