Fig 1: Targeted HSPCs Repopulate the Immune Cell Lineages(A) Experimental scheme of mCherry insertion into the Lmnb1 gene in HSPCs that were transplanted into irradiated Rag2-/-c?-/- mice. Hematopoietic cell lineages were analyzed by flow cytometry 8 weeks (n = 4) or 16 weeks (n = 3) after reconstitution.(B) FACS analysis showing the percentages of donor cells (GFP+) in the bone marrow (BM) and spleen of the recipient mice 8 weeks after reconstitution.(C) Frequency of mCherry+ cells among GFP- (host) or GFP+ (donor) cells in the bone marrow and spleen of the recipients determined by flow cytometry (left). The graph depicts percentages of mCherry+ cells in the bone marrow (black) and spleen (orange) of the recipient animals 8 weeks (n = 4) and 16 weeks (n = 3) after reconstitution.(D) LSK gating (left) and frequency of the mCherry+ cells among the LSK cells 8 and 16 weeks after reconstitution (right). The graph (right) shows the data from 4 recipient animals (8 weeks) or 3 recipient animals (16 weeks).(E) Absolute numbers of mCherry+ T, B, NK, and Gr-1+ cells in the bone marrow and spleen of the recipient animals 8 weeks (black) or 16 weeks (orange) after reconstitution.(F) FACS analysis showing frequencies of mCherry+ cells among the HSC/MPP1, MPP2, and MPP3/4 LSK subpopulations in the bone marrow of the recipient mice 8 and 16 weeks after reconstitution.(G) Graphs depict percentages of LSK subsets (top) and percentages of mCherry+ cells within each subset (bottom).