Fig 2: Flow cytometric analysis of uNK cell subset in rat endometrium on D6, D8, and D10 of pregnancy. (a) The representative graphs of 2-color flow cytometric analysis of uNK cell subset (gated to lymphocytes). (b) Statistical analysis of uNK (CD3-CD161+) cell subset in rat endometrium. The percentage of uNK cell subset was presented as mean ± SD (n = 6). *P < 0.05, **P < 0.01 compared to N; # P < 0.05, ## P < 0.01 compared to group M.

Fig 3: Limb remote ischemic preconditioning (RIPC) increases the splenic lymphocyte populations. RIPC was conducted by four cycles (10 min/cycle, 40 min total) of bilateral hindlimb ischemia. Spleens were removed before RIPC and 1 h and 3 days after RIPC. a Representative images of flow cytometry showing different splenic lymphocytes, including helper T (Th) cells (CD3+CD4+), cytotoxic T (Tc) cells (CD3+CD8+), natural killer T (NKT) (CD3+CD161+) cells, and B (CD3-D45RA+). Indicated numbers are the mean percentages of targeted cells. b Statistical analysis of splenic lymphocyte populations prior to and after RIPC. n = 6 rats per group. Data are presented as means ± SEM, *p < 0.05, **p < 0.01 versus corresponding lymphocytes before RIPC

Fig 4: Study on remodeling of Lipo@GB-DHA in vivo. (A) Gating strategy of flow cytometry analysis for macrophages and microglia in brains. (B-D) Representative flow cytometry images (B) and quantifications (C-D) of CD11b+CD45high macrophage and CD11b+CD45med microglia in brains (n = 6). (E-F) Representative flow cytometry image (E) and quantifications (F) of M1/M2 cells in CD11b+CD45med microglia in the ipsilateral side (n = 6). (G-H) Representative flow cytometry images (G) and quantification (H) of the proportion of CD3+ lymphocytes in the ipsilateral side (n = 6). (I) Immunostaining of CD206 and Iba-1 to determine the microglial phenotype in the ischemic penumbra treated with different formulations (Scale bar: 200 µm/20 µm). (J) Western blot analysis of autophagy-related proteins in brains. Data in (D-E, F-H) were expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ****P < 0.0001.