Fig 2: IL-6 inhibits anti-PD-L1 efficacy and anti-tumor CTL response(A–H) Treatment of EMT6 tumor-bearing mice with antibodies targeting PD-L1 and/or IL6R. (A) Individual tumor growth curves (n = 10 per group) from one of three independent studies. PD, progressive disease; PR, partial response; CR, complete response. (B) Progression-free survival (time to 5x increase in tumor volume) of mice pooled from three independent studies, analyzed using log rank test. (C) Cellular composition of CD45+ tumor-infiltrating leukocytes, from one of three experiments. (D) Balance of CD4+ and CD8+ T cells (CTLs) among total TCRß+ tumor-infiltrating T cells. Data are concatenated from n = 5 mice per group, from one of three studies. (E) Relative abundance (normalized to tumor weight) of CTLs, conventional CD4+ T cells, and regulatory (CD4+ Foxp3+) T cells in EMT6 tumors, pooled from three experiments. Groups compared using one-way ANOVA with Holm-Sidak’s multiple comparisons test. (F) Effector phenotype of tumor-infiltrating CTLs after re-stimulation with PMA/ionomycin. (G) Frequencies of polyfunctional cells among tumor-infiltrating CTLs (left panel), and their absolute abundance relative to isotype control (right panel). Data pooled from three experiments. Groups compared using one-way ANOVA with Holm-Sidak’s multiple comparisons test. (H) Relative abundance (mean ± SEM) of polyfunctional CTLs and IFN-?+TNF+ CD4+ T cells (multifunctional cells) vs. dysfunctional cells (IFN-?- TNF- GzmB- CD8+ T cells, or IFN-?- TNF- CD4+ T cells), from one of three experiments with n = 3–5 per group. Groups compared using one-way ANOVA with Holm-Sidak’s multiple comparisons test. *p = 0.0143, ***p = 0.0001, ****p < 0.0001.(I–K) Single-cell RNA-seq analysis of pre-treatment peripheral blood CD8+ T cells from patients with RCC (from IMmotion150) or UC (from IMvigor210). Differential expression analysis was performed on CD8+ T cells from patients with high (>10 pg/mL) versus low plasma IL-6 (separate analyses for each cancer type). Genes identified as concordant in both cohorts (“consensus genes”) were evaluated for Reactome pathway enrichment (I). Volcano plots of differential gene expression are shown for UC and RCC samples in (J) and (K), respectively.

Fig 3: CTL-intrinsic IL-6 signaling impairs anti-PD-L1 efficacy(A) IL6R expression on lymph node T cells from CD8?IL6R mice or WT littermate controls (n = 5 mice per group).(B) Tumor growth (mean +/- SEM) in CD8?IL6R mice and WT littermates treated with anti-PD-L1 or control antibodies, pooled from two independent studies and compared using two-way ANOVA.(C–F) Cytokine expression in tumor-infiltrating CTLs after 1 week of treatment. Representative staining and Boolean analysis are shown in (C) and (D), respectively. Frequencies of IFN-?+ TNF+ cells among tumor-infiltrating CTLs (E), and their absolute number (F), compared using one-way ANOVA with Holm-Sidak’s multiple comparisons test (n = 9–12 mice per group). Data pooled from two independent studies.(G–I) RNA-seq analysis of FACS-purified tumor-infiltrating CTLs from CD8?IL6R mice or WT littermates. Mice with established MC38 tumors (~150 mm3) were treated with anti-PD-L1 or control antibodies for 7 days (n = 4–5 mice per group). (G) Differentially expressed genes between WT and CD8?IL6R CTLs. Separate comparisons were made based on treatment. (H) Reactome pathway analysis of anti-PD-L1-driven protein-coding genes (p < 0.05) in WT and CD8?IL6R CTLs. (I) Reactome pathway analysis of the top 500 protein-coding genes (ranked by p value) that were significantly associated with CD8?IL6R CTLs during anti-PD-L1 treatment.

Fig 4: IL-6 blocks CTL effector differentiation(A) Splenocytes from OT-I mice were stimulated with SIINFEKL peptide +/- IL-6 and analyzed by flow cytometry on day 7. Boolean analysis of IFN-?, TNF, and GzmB expression in CTLs is shown. Groups (mean +/- SEM of n = 4 technical replicates) were compared by t test and represent one of three independent experiments.(B) Cytokine secretion (measured by Luminex multiplex assay) by FACS-purified CTLs activated with anti-CD3/CD28 +/- IL-6 for 3 days *p < 0.05 (t test; n = 3 technical replicates per condition).(C) OT-I CTLs were activated as described in (A) and co-cultured with SIINFEKL-pulsed MC38.GFP cells at a 5:1 effector/target ratio. MC38 destruction was quantified using Incucyte live-cell imaging. Groups compared using two-way ANOVA (n = 4 technical replicates per condition); data indicate mean ± SEM, and are representative of three independent experiments.(D–I) OT-I splenocytes activated with SIINFEKL peptide in the presence of IL-6, control IgG, or anti-IL6R antibody. CTLs were FACS-purified for RNA-seq analysis after 2 and 7 days (n = 3 technical replicates per condition/time point). Principal components analysis (PCA) is shown in (D). (E) Differentially expressed genes (FDR <0.05 and absolute fold change >1.5). (F) Heatmap of representative differentially expressed genes (day 7). (G) Boolean flow cytometry analysis of OT-I CTLs at day 7. Groups (mean +/- SEM of n = 4 technical replicates) compared using t tests, from one of three independent experiments. (H) Reactome pathway analysis of differentially expressed genes between IL-6- and anti-IL6R-treated cells at day 7. (I) Distribution of differentially expressed genes (day 7) among differentiation modules from Best et al.25 *p = 0.0239, ****p < 0.0001 (Fisher’s exact test). Fold differences refer to IL-6 versus anti-IL6R treatment.(J) Tumor RNA-seq analysis from IMmotion150. Gene modules (average Z scores) associated with effector or naive-like CTLs were used to calculate an effector/naive-like (Eff/N) ratio. IL6 expression across Eff/N quartiles (mean +/- SEM, n = 65–66 per group) was compared using one-way ANOVA.