Fig 1: Expression levels of members of the PLIN family in cardiac tissues. A: Western blot analysis of PLIN1, PLIN2, PLIN3, and PLIN5 in the cardiac ventricles of Wt, AKO, cHSL, and AKO+cHSL (A+H) mice: 20 µg of protein sample was subjected to SDS-PAGE, transferred to nitrocellulose membranes, and probed with an appropriate antibody. Four mice per group were analyzed, and the representative blots are presented. Note that PLIN1 was detected after an extremely long exposure. The graphs present the densitometric analysis of protein expression normalized to that of GAPDH. Values are presented as the mean ± SEM of four mice/group. B: mRNA expression levels of PLIN1, PLIN2, PLIN3, and PLIN5 in the cardiac ventricles of Wt, AKO, cHSL, and AKO+cHSL (A+H) mice. The expression levels were determined via RT-qPCR and normalized to GAPDH expression. Values are presented as the mean ± SEM of four mice per group. *P < 0.05, **P < 0.01 versus Wt mice; †P < 0.05, ††P < 0.01 versus AKO mice.