APC anti-mouse CD4 100412 from BioLegend

Product Specs
ItemAPC anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100412
NCBI Gene AliasesL3T4, Ly-4
IsotypeIgG2b, Kappa
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
ReactivityMouse
CloneGK1.5
HostRat
Quantity100 µg
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenMouse CTL clone V4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) DNA-scaffolded multivalent vaccine against SARS-CoV-2Acta BiomaterialiaJuly 1, 2023Fangfang Chen, Yuhan Huang, Zhengyu Huang, Tingting Jiang, Zailin Yang, Jie Zeng, Aishun Jin, Hua Zuo, Cheng Zhi Huang, Chengde MaoELISpot Kit were purchased from Dakewe Biotech Co., Ltd. (Shenzhen, China). APC antimouse CD4 (Cat# 100411), FITC antimouse CD8a (Cat# 100705), PerCP antimouse CD3ε (Cat# 100325), PE antimouse CD44 (Cat# 103007),

(2) Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flowCell reportsJune 27, 2023Kamioka Y, Ueda Y, Kondo N, Tokuhiro K, Ikeda Y, Bergmeier W, Kinashi T.Armenian Hamster anti-mouse CD3ϵ,Biolegend,Cat# 100312; RRID: AB_312677 APC Rat anti-mouse CD4,Biolegend,Cat# 100411; RRID: AB_312696 APC Rat anti-mouse CD8a,Biolegend,Cat# 100712; RRID: AB_312751 Biotin Rat anti-mouse

(3) Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesiseLifeJune 2, 2023Nakajima-Takagi Y, Oshima M, Takano J, Koide S, Itokawa N, Uemura S, Yamashita M, Andoh S, Aoyama K, Isshiki Y, et al.RRID:AB_2621556,FACS (5 × 10–3 μL/1 × 106 cell) Antibody,APC anti-mouse CD4 antibody (rat monoclonal),BioLegend,Cat# 100412; RRID:AB_312697,FACS (0.1 μL/1 × 106 cell) Antibody,APC/Cyanine7 anti-mouse CD4 antibody (rat monoclonal),BioLegend,Cat#

(4) An enzyme-responsive and transformable PD-L1 blocking peptide-photosensitizer conjugate enables efficient photothermal immunotherapy for breast cancerBioactive materialsApril 1, 2023Sun Y, Lyu B, Yang C, He B, Zhang H, Wang X, Zhang Q, Dai W.antibody (cat# 124307), FITC anti-mouse CD8 antibody (cat# 100706), APC anti-mouse CD4 antibody (cat# 100412), FITC anti-mouse CD11c antibody (cat# 117305), PE anti-mouse CD80 antibody (cat# 104707) and APC anti-mouse CD86

(5) Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelinationJournal of neuroinflammationFebruary 18, 2023Wang Y, Sadike D, Huang B, Li P, Wu Q, Jiang N, Fang Y, Song G, Xu L, Wang W, et al.biosciences, Cat# 553,141) for 15 min at 4 ℃ and then incubated with CD4-APC (1:100, Biolegend, Cat# 100411) for 30 min at 4 ℃. Data were acquired on CytoFLEX3 (Beckman Coulter, USA) and analyzed with Flowjo

(6) RIPK3 controls MAIT cell accumulation during development but not during infectionCell death & diseaseFebruary 11, 2023Patton T, Zhao Z, Lim XY, Eddy E, Wang H, Nelson AG, Ennis B, Eckle SBG, Souter MNT, Pediongco TJ, et al.α-galactosylceramide loaded CD1d tetramers (PE, provided courtesy of Dale Godfrey), anti-mouse CD4-APC (BioLegend #100412; Clone GK1.5, 1/200), anti-mouse CD4-BV786 (BD #563331; Clone GK1.5, 1/200), anti-mouse CD8α−PE (Invitrogen

(7) Acute murine cytomegalovirus infection boosts cell-type specific response and lipid metabolism changes in the liver of infant miceFrontiers in immunologyJanuary 1, 2023Gao J, Wang A, Bu X, Jia W.108710)), Fixable Viability Dye (Biolegend, 423102), CD3-FITC (Biolegend, 100204), CD4-APC (Biolegend, 100412), Ly6G-PE (Biolegend, 127608), Ly-6C-PerCP-Cy5.5 (Biolegend, 128012), CD11b-PE/Cy7 (Biolegend, 101216).

(8) Antibody-Mediated Delivery of VEGF-C Promotes Long-Lasting Lymphatic Expansion That Reduces Recurrent InflammationCellsDecember 31, 2022Cousin N, Bartel S, Scholl J, Tacconi C, Egger A, Thorhallsdottir G, Neri D, Dieterich LC, Detmar M.populations, cells were stained with anti CD45-PacificBlue (103216, Biolegend, 1:400), anti CD4-APC (100412, Biolegend, 1:400), anti γδ-TCR-PerCp AF700 (100714, eBioscience, San Diego, CA, USA, 1:400), anti CD8-APC/Cy7

(9) LSD1 deletion decreases exosomal PD-L1 and restores T-cell response in gastric cancerMolecular CancerDecember 1, 2022Dan-Dan Shen et al.suspension was stained with PE-CY7-CD45 (103114, Biolegend, USA), APC-CD3 (300312, Biolegend, USA), APC-CD4 (100412, Biolegend, USA), PE-CD8a (100708, Biolegend, USA) for 40 min. Finally, the stained cells were analyzed

(10) MDSCs participate in the pathogenesis of diffuse pulmonary hemorrhage in murine lupus through mTOR-FoxO1 signalingBiochemistry and biophysics reportsDecember 1, 2022Tan L, Shi G, Zhao J, Xia X, Li D, Wang S, Liang J, Hou Y, Dou H.594 (Cat. No. 155529, clone S17007L), CD11c-APC (Cat. No. 117310, clone N418) and CD4-APC (Cat. No. 100412, clone GK1.5) (all from Biolegend), FoxO1-Alexa Fluor 532 (Cat. No. NBP2-31376AF532, clone 83N7F8) or

(11) Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumorsNature CommunicationsDecember 1, 2022Fotis Nikolos et al.antibodies: CD45-BV570 (Biolegend, 103135, 1:400); CD3-PE (Biolegend, 100206, 1:200); CD4-APC (Biolegend, 100411, 1:200); and CD8-FITC (Biolegend, 100706, 1:200), Tbet-BV711 (Biolegend, 644819, 1:200), IFNg-BV785

(12) Magnesium galvanic cells produce hydrogen and modulate the tumor microenvironment to inhibit cancer growthNature CommunicationsDecember 1, 2022Nailin Yang et al.Anti-CD3-FITC (Biolegend, clone 17A2, Catalog: 100204), anti-CD4-APC (Biolegend, clone GK1.5, Catalog: 100412), anti-CD8-PE (Biolegend, clone 53–6.7, Catalog: 100708), anti-CD45-FITC (Biolegend, clone I3/2.3, Catalog:

(13) Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channelsJournal of extracellular vesiclesDecember 1, 2022Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, et al.Antibody,"BioLegend, San Diego, CA, USA",100218, APC Anti‐Mouse CD4 Antibody,"BioLegend, San Diego, CA, USA",100412, PE/Cyanine7 Anti‐Mouse CD8a Antibody,"BioLegend, San Diego, CA, USA",100722, PE Anti‐Mouse CD366 (Tim‐3

(14) A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γCell reportsNovember 22, 2022Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.antibodies of FITC anti-mouse CD3 antibody (BioLegend, #100204), APC anti-mouse CD4 antibody (BioLegend, #100412), and Brilliant Violet 711™ anti-mouse CD8a antibody (BioLegend, #100747). METHOD DETAILS Bioinformatic

(15) Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasisCell Reports MedicineNovember 15, 2022Rebeca Uceda-Castro, Andreia S. Margarido, Lesley Cornet, Serena Vegna, Kerstin Hahn, Ji-Ying Song, Diana A. Putavet, Mariska van Geldorp, Ceren H. Çitirikkaya, Peter L.J. de Keizer, et al.RRID:AB_2566568 Anti-mouse CD45 Alexa Fluor 700,Biolegend,103123; RRID:AB_493534 Anti-mouse CD4 APC,Biolegend,100412; RRID:AB_312697 Anti-mouse CD8 FITC,eBioscience,11-0081-82; RRID:AB_464915 Anti-mouse NK1.1,Biolegend,108713;

(16) T cells specific for α-myosin drive immunotherapy-related myocarditisNatureNovember 1, 2022Axelrod ML, Meijers WC, Screever EM, Qin J, Carroll MG, Sun X, Tannous E, Zhang Y, Sugiura A, Taylor BC, et al.dilution 1:400), CD3-AF488 (BioLegend, cat# 100210, clone 17A2, dilution 1:200), CD4-APC (BioLegend, cat# 100412, clone GK1.5, dilution 1:100), CD8a-PE/Cy7 (BioLegend, cat# 100722, clone 53-6.7, dilution 1:400), Thy1.1

(17) The proprotein convertase furin regulates the development of thymic epithelial cells to ensure central immune toleranceiScienceOctober 21, 2022Liang Z, Zhang Z, Zhang Q, Dong X, Yang X, Zhang J, Lei T, Creemers JWM, Zhang B, Zhao Y.RRID:AB_2650896 Anti-CD40-PE,BioLegend,Cat#124610; RRID:AB_1134075 Anti-CD4-FITC,BioLegend,Cat#100406; RRID:AB_312691 Anti-CD4-APC,BioLegend,Cat#100412; RRID:AB_312697 Anti-CD4-APC/Cy7,BioLegend,Cat#100414; RRID:AB_312699 Anti-CD8a-BV421,BioLegend,Cat#100738;

(18) Engineered human cytokine/antibody fusion proteins expand regulatory T cells and confer autoimmune disease protectionCell reportsOctober 18, 2022VanDyke D, Iglesias M, Tomala J, Young A, Smith J, Perry JA, Gebara E, Cross AR, Cheung LS, Dykema AG, et al.1:1200), anti-mouse CD3 BV605 (17A2, BioLegend 100237, 1:80), anti-mouse CD4 APC (GK1.5, BioLegend 100411, 1:3200), anti-mouse CD8a BV786 (53–6.7, BD Biosciences 563332, 1:400), anti-mouse IL-2Rα BV421 (7D4,

(19) Immunization with a Prefusion SARS-CoV-2 Spike Protein Vaccine (RBMRNA-176) Protects against Viral Challenge in Mice and Nonhuman PrimatesVaccines (Basel)October 11, 2022Qinhai Ma, Runfeng Li, Jianmin Guo, Man Li, Lin Ma, Jun Dai, Yongxia Shi, Jinlong Dai, Yuankeng Huang, Cailing Dai, et al.100218), APC anti-mouse CD8a antibody (BioLegend, 100712), and APC anti-mouse CD4 antibody (BioLegend, 100412) were used for surface staining. PE anti-mouse/human IL-5 antibody (BioLegend, 504204), PE anti-mouse IL-2

(20) Absence of TSC1 Accelerates CD8⁺ T cell-mediated Acute Cardiac Allograft RejectionAging and diseaseOctober 1, 2022Liang Tan, Yanan Xu, Gongbin Lan, Hongxia Wang, Zhanfeng Liang, Zhaoqi Zhang, Qianchuan Tian, Yangxiao Hou, Yong Zhao, Xubiao XieBioLegend), anti-mCD4-PE-Cy5 (clone GK1.5, Cat#:100410; BioLegend), anti-mCD4-APC (clone GK1.5, Cat#:100412; Biolegend), anti-mCD4-APC-Cy7 (clone GK1.5, Cat#: 100412; BioLegend), anti-mCD44-fluorescein isothiocyanate

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(1)

3/15/2019Staining Mouse Splenocytes with Anti-CD4 APC Adam GuessThis antibody was used as a marker for CD4+ T cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, APC is bright and works well with other fluorophores in the ... Read Review
(1)

2/25/2019Mouse CD4 Antibody for Flow Cytometry Matthew RatsepI use this antibody for staining mouse lymphocytes collected from blood, spleen, or lungs. It works well, gives a good signal, and is easy to optimize.
(1)

1/30/2015APC anti-mouse CD4 Antibody for CD4 staining Wei CaoMouse total T cells were collected and activated by anti-CD3/CD28 antibody (hamster derived) in vitro (pre-coated with goat anti-hamster antibody) for 3 days and stained with this antibody. During our... Read Review

APC anti-mouse CD4 from BioLegend

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APC anti-mouse CD4 from BioLegend

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